MRTFA (NM_001282661) Human Tagged ORF Clone

CAT#: RC239727

  • TrueORF®

MKL1 (myc-DDK-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001282661" in other vectors (2)

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CNY 7,130.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


MKL1 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BSAC; MAL; MKL; MKL1; MRTF-A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC239727 representing NM_001282661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAGG
ACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAAGA
GACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGACCTC
AATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAGTCCA
GCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTCGATGA
GGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCGTCACCC
CTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGCAAAGCCAACCCA
AGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGAAGAAGCTCAA
GTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCCCCCATGGACTCATCCTACGCC
AAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAACCAGCAGCAGCAGCAGCACCACA
ACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCCCTGGGAAGCAGCGGGACCCCCCC
AGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCCCCTGGGCCCTGTGGGCTGGCACGT
CAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCCAACCTGGACGACATGAAGGTGGCAG
AGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGCACCAAAACTGAGCTGATTGAGCGCCT
TCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCCAAGGCCCCTGCCGCCACCTCTATCCTG
CACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGGCTGAGCACGGGGCCAGCCCTGGTGGCAG
CAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCCAGCAGTGGGGTGGTGAAGTTTGGCAGCAC
GGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGCTCACTGCTCAGCACGGGCGATGAAAACTCC
ACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCTCTGACGCAGCTGACCCTGCAGGCCTCGCCAC
TGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGGTCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGA
GCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAGAAAGACAAGCAGATCGAGGCGCTGACGCGCATG
CTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAGCTGCAGCTGGAGCAGGAGAAGCGAGCCCAGCAGC
CCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTGAAGCAGGAGAACAGCTTCTCCAGCTGCCAGCTGAG
CCAGCAGCCCCTGGGCCCCGCTCACCCATTCAACCCCAGCCTGGCGGCCCCAGCCACCAACCACATAGAC
CCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTGGTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGC
CGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCTCAGGGCCCCAGCCTCATCAAGGGGGTTGCACCTCCCAC
CCTCATCACCGACTCCACAGGGACCCACCTTGTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGC
CTGTCCAGTGGGAGCCCCCAGCAGCCCTCGTCCCAGCCTGGCTCTCCAGCGCCTGCCCCCTCTGCCCAGA
TGGACCTGGAGCACCCACTGCAGCCCCTCTTTGGGACCCCCACTTCTCTGCTGAAGAAGGAACCACCTGG
CTATGAGGAAGCCATGAGCCAGCAGCCCAAACAGCAGGAAAATGGTTCCTCAAGCCAGCAGATGGACGAC
CTGTTTGACATTCTCATTCAGAGCGGAGAAATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGA
AGGAGAAGCCATCCCCGAAGACAGTCTGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGCTGAGCT
CCCCCAGGCTGCCCCACCTCCTCCAGGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTTCCTGGAGAGC
AGCACGGGGCTGCCCCTGCTGACCAGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATTGACGACCTCC
ATAGCCAGATGCTGAGCAGCACTGCCATCCTGGACCACCCCCCGTCACCCATGGACACCTCGGAATTGCA
CTTTGTTCCTGAGCCCAGCAGCACCATGGGCCTGGACCTGGCTGATGGCCACCTGGACAGCATGGACTGG
CTGGAGCTGTCGTCAGGTGGTCCCGTGCTGAGCCTAGCCCCCCTCAGCACCACAGCCCCCAGCCTCTTCT
CCACAGACTTCCTCGATGGCCATGATTTGCAGCTGCACTGGGATTCCTGCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC239727 representing NM_001282661
Red=Cloning site Green=Tags(s)

MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADDL
NEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSP
LEARVSEPLLSATSASPTQQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYA
KILQQQQLFLQLQILNQQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLAR
QNSTSLTGKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSIL
HKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENS
TPGDTFGEMVTSPLTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRM
LRQKQQLVERLKLQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHID
PCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPG
LSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDD
LFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLES
STGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDW
LELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWDSCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001282661
ORF Size 2643 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001282661.1, NP_001269590.1
RefSeq Size 4400 bp
RefSeq ORF 2946 bp
Locus ID 57591
UniProt ID Q969V6
Protein Families Transcription Factors
MW 94 kDa
Gene Summary The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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