DNAH2 (NM_001303270) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DNAHC2; DNHD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239715 representing NM_001303270 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCAAAGCTGAGAAGAAGCAGCGATTGAGTGGCCGAGGAAGCTCCCAGGCAAGCTGGTCAGGGC GGGCCACTCGGGCTGCTGTGGCCACACAGGAGCAGGGGAATGCCCCGGCTGTCAGTGAGCCAGAGCTGCA GGCTGAGCTCCCCAAGGAGGAGCCTGAGCCACGGTTGGAGGGACCTCAAGCACAGAGTGAAGAATCAGTG GAGCCCGAGGCAGATGTGAAGCCCCTCTTCCTTTCCCGAGCTGCGCTGACAGGACTGGCGGATGCAGTGT GGACACAGGAGCATGATGCCATTCTGGAACACTTTGCCCAGGACCCTACAGAATCCATCCTCACCATCTT CATTGACCCTTGTTTTGGGCTGAAGCTAGAGCTGGGCATGCCTGTACAGACCCAGAACCAGCTTGTCTAC TTCATTCGCCAAGCACCAGTTCCCATCACCTGGGAGAACTTCGAGGCAACTGTGCAGTTTGGGACGGTGC GGGGCCCCTATATCCCGGCCCTGCTTCGGCTGCTCGGTGGAGTCTTTGCCCCTCAGATCTTTGCAAACAC AGGCTGGCCTGAGAGCATTAGAAATCATTTTGCTTCTCATCTGCACAAGTTCTTGGCCTGCCTGACAGAC ACTCGGTACAAACTGGAGGGGCACACGGTCCTCTACATCCCTGCAGAGGCCATGAACATGAAGCCTGAGA TGGTGATAAAGGACAAAGAGCTGGTGCAACGGCTAGAGACCTCCATGATCCACTGGACCCGGCAGATAAA GGAGATGCTCAGTGCCCAGGAGACTGTGGAGACAGGAGAAAATTTAGGTCCTCTGGAGGAGATTGAGTTC TGGCGCAACCGATGCATGGACCTGTCTGGCATCAGTAAGCAGCTGGTGAAGAAGGGAGTGAAGCACGTTG AATCCATCCTGCACCTTGCCAAGTCGTCCTACTTGGCGCCCTTTATGAAACTGGCACAGCAGATCCAGGA TGGCTCTCGTCAAGCACAGTCAAACCTGACCTTTTTGTCAATCCTGAAGGAACCTTACCAGGAGTTGGCT TTCATGAAGCCCAAGGACATCTCTAGCAAGCTCCCTAAGCTGATCAGTCTCATCCGCATCATCTGGGTCA ACTCTCCCCACTACAACACTCGGGAGAGACTGACCTCGCTCTTCCGAAAGATGAGCAATGAGATCATCCG CTTATGCTGCCACGCCATCTCCCTGGACCGGATCTTTGAGGGATATGTCTCTTCCAGCAAGGAGGACCTG CAAGGCTGCATTCTCTGTTGTCACGCTTGGAAAGATCACTACGTACAGGCTGTGCAGATGCACATCCAGT TCTCCAGTCGGGGCTGGGTCCTAGATCAGACCAGCATCTTTGCTCAGGTTGATGCCTTTGTGCAGCGCTG CAAGGACCTTATTGAGGTATGTGACTGTCAGTATCACTTCGCCCGCTGGGAAGATGGCAAGCAGGGTCCC CTTCCTTGCTTCTTTGGTGCCCAGGGGCCACAGATAACACGGAACTTGCTGGAGATTGAGGACATCTTTC ATAAAAATCTGCACACGCTGCGAGCCGTTCGCGGGGGTATCCTGGATGTCAAGAACACCTGTTGGCATGA AGACTACAATAAGTTCCGTGCCGGAATCAAGGACCTGGAGGTGATGACCCAGAACCTGATCACCTCAGCC TTCGAGTTGGTGCGGGACGTGCCGCACGGCGTGCTTCTGCTGGACACCTTCCACAGGCTTGCCTCCCGCG AGGCTATCAAGCGGACTTATGACAAGAAGGCGGTGGATCTCTACATGCTGTTCAATAGCGAGCTGGCCCT GGTGAACCGTGAACGGAACAAGAAATGGCCAGACCTGGAGCCCTACGTGGCCCAGTATTCCGGAAAGGCG CGCTGGGTGCACATCCTCCGGCGTCGCATCGACAGAGTCATGACCTGCCTTGCTGGTGCTCATTTCCTGC CCCGTATTGGGACTGGAAAGGAGAGTGTGCACACCTATCAGCAGATGGTCCAGGCCATTGATGAGCTGGT TCGAAAAACCTTCCAAGAGTGGACATCAAGTCTGGACAAGGATTGCATTCGGCGGTTGGATACCCCATTG CTGCGAATCAGCCAGGAGAAGGCGGGCATGCTGGATGTCAACTTTGACAAGTACAGGAGCCACCTGGCCC CTTTTCCCTATACTCCCCTTCTGCAGCTCTCCCAAGAATTTCACTCCCATCTTTTGACTCCTCTCTTCAT TATTCTCTCTCTTTCTCATACAATATGTTTGCTATCGTCATTTTACTTTTTTTTTTCTTCTTTTATTTTT GTCTCTCCCCACCTCCCACCCTGCTATCAACATTTTAATTTTACAACTTATCTTAAAACACAACAAAACA AAACAATGATCGGCCAGGCACGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCGC ATCGCTTGAGCCCAGGAGTTTGAGAACAGCCTGGGCAACATGGCAAAACCCAGTCTCTGCAAAAAATACA AAGATTAGCTGGGCGTGGTGGCACAAGCCTGTAGTCTCAGCTACTTGGGAGGGTGAGGTGGGAGGATCGC CTGAGCCCGGGAGGCAGAGGCTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239715 representing NM_001303270 Red=Cloning site Green=Tags(s) MSSKAEKKQRLSGRGSSQASWSGRATRAAVATQEQGNAPAVSEPELQAELPKEEPEPRLEGPQAQSEESV EPEADVKPLFLSRAALTGLADAVWTQEHDAILEHFAQDPTESILTIFIDPCFGLKLELGMPVQTQNQLVY FIRQAPVPITWENFEATVQFGTVRGPYIPALLRLLGGVFAPQIFANTGWPESIRNHFASHLHKFLACLTD TRYKLEGHTVLYIPAEAMNMKPEMVIKDKELVQRLETSMIHWTRQIKEMLSAQETVETGENLGPLEEIEF WRNRCMDLSGISKQLVKKGVKHVESILHLAKSSYLAPFMKLAQQIQDGSRQAQSNLTFLSILKEPYQELA FMKPKDISSKLPKLISLIRIIWVNSPHYNTRERLTSLFRKMSNEIIRLCCHAISLDRIFEGYVSSSKEDL QGCILCCHAWKDHYVQAVQMHIQFSSRGWVLDQTSIFAQVDAFVQRCKDLIEVCDCQYHFARWEDGKQGP LPCFFGAQGPQITRNLLEIEDIFHKNLHTLRAVRGGILDVKNTCWHEDYNKFRAGIKDLEVMTQNLITSA FELVRDVPHGVLLLDTFHRLASREAIKRTYDKKAVDLYMLFNSELALVNRERNKKWPDLEPYVAQYSGKA RWVHILRRRIDRVMTCLAGAHFLPRIGTGKESVHTYQQMVQAIDELVRKTFQEWTSSLDKDCIRRLDTPL LRISQEKAGMLDVNFDKYRSHLAPFPYTPLLQLSQEFHSHLLTPLFIILSLSHTICLLSSFYFFFSSFIF VSPHLPPCYQHFNFTTYLKTQQNKTMIGQARWLTPVIPALWEAEVGASLEPRSLRTAWATWQNPVSAKNT KISWAWWHKPVVSATWEGEVGGSPEPGRQRLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303270
ORF Size	2616 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303270.2
RefSeq Size	3006 bp
RefSeq ORF	2619 bp
Locus ID	146754
UniProt ID	Q9P225
Protein Pathways	Huntington's disease
MW	100.4 kDa
Gene Summary	Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAH2 is an axonemal inner arm dynein heavy chain (Chapelin et al., 1997 [PubMed 9256245]).[supplied by OMIM, Mar 2008]

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