CRNKL1 (NM_001278625) Human Tagged ORF Clone
CAT#: RC239653
- TrueORF®
CRNKL1 (myc-DDK-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 2
ORF Plasmid: tGFP
"NM_001278625" in other vectors (2)
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CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CLF; Clf1; CRN; HCRN; MSTP021; SYF3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239653 representing NM_001278625
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGACAAAAACAGGC GCCACGGCTCGACAGAGCCACTCGTCCTCGCCTGGTCATCCCAGTTCCGGAGGCTGACCTGGGGATGCGC CCTGGACGCCCTGCACCGCAGTCCTTGCGTCGCTGCTAGTCAGCACGGCGTTACCCACCTCATCCGCAGC TCCAGGACGCCGCACTCCACGCGCTGCCGCAAGGAGGACGCGCAGCCTGGTCACCATGGAAACGGTGCCG CCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTCAGTCCTGCAAGTTCCGCTTCCGGTTCCGAGATCCTG TTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGCTCTCAGAGCCGATTTCTAGCGTCCGTGCCGGGGACA GGTGTCCAGAGGTCGACTGCTGCAGACATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAG TGGCCAAGGTGAAAAACAAAGCCCCGGCTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAA AGAAAGAGAACTTGAGCTTCTTCCACCTCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGAT TATAAACTAAGGAAAAGGAAGACTTTTGAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGA TAAAATACGCACAATGGGAAGAAAGCCTAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTT AGATGTAGACTACCGAAATATTACACTCTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTC AACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGT ACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTG GCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCC CGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTG AAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGA ACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTA CGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATA CCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCA GTACGAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAA AGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGG AGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAA GGATCCTGAGAGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTT GCCAAAATGTGGATACTGTATGCACAGTTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCAT TGGGAACTTCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCT TCGAGAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCA TGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAG CCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCA GGAAGAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGG ATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATG AAGAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCG AAGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTC AAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCT TTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCA GCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGATGAGAGTGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239653 representing NM_001278625
Red=Cloning site Green=Tags(s) MTATVENLTFQKDTLGNAVDKNRRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIRS SRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVPGT GVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELND YKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQV NHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRA RTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERV RVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVE SDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTF AKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTS WIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVW ISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKV KKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278625 |
ORF Size | 2508 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001278625.1, NP_001265554.1 |
RefSeq Size | 4377 bp |
RefSeq ORF | 2511 bp |
Locus ID | 51340 |
UniProt ID | Q9BZJ0 |
Protein Pathways | Spliceosome |
MW | 99.6 kDa |
Gene Summary | The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] |
Documents
Product Manuals |
FAQs |
SDS |