MIA3 (NM_001300867) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARNT; D320; TANGO; TANGO1; UNQ6077
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239559 representing NM_001300867 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCAGTACCTGCCACTGTGCCTTCTATCGCCGCTACCCCGGGGGACCCGGAACTTGTGGGACCCT TGTCTGTGCTCTACGCAGCCTTCATAGCCAAGCTGCTGGAGCTAGTTGCTACATTGCCTGATGATGTTCA GCCTGGGCCTGATTTTTATGGACTGCCATGGAAACCTGTATTTATCACTGCCTTCTTGGGAATTGCTTCG TTTGCCATTTTCTTATGGAGAACTGTCCTTGTTGTGAAGGATAGAGTATATCAAGTCACGGAACAGCAAA TTTCTGAGAAGTTGAAGACTATCATGAAAGAAAATACAGAACTTGTACAAAAATTGTCAAATTATGAACA GAAGATCAAGGAATCAAAGAAACATGTTCAGGAAACCAGGAAACAAAATATGATTCTCTCTGATGAAGCA ATTAAATATAAGGATAAAATCAAGACACTTGAAAAAAATCAGGAAATTCTGGATGACACAGCTAAAAATC TTCGTGTTATGCTAGAATCTGAGAGAGAACAGAATGTCAAGAATCAGGACTTGATATCAGAAAACAAGAA ATCTATAGAGAAGTTAAAGGATGTTATTTCAATGAATGCCTCAGAATTTTCAGAGGTTCAGATTGCACTT AATGAAGCTAAGCTTAGTGAAGAGAAGGTGAAGTCTGAATGCCATCGGGTTCAAGAAGAAAATGCTAGGC TTAAGAAGAAAAAAGAGCAGTTGCAGCAGGAAATCGAAGACTGGAGTAAATTACATGCTGAGCTCAGTGA GCAAATCAAATCATTTGAGAAGTCTCAGAAAGATTTGGAAGTAGCTCTTACTCACAAGGATGATAATATT AATGCTTTGACTAACTGCATTACACAGTTGAATCTGTTAGAGTGTGAATCTGAATCTGAGGGTCAAAATA AAGGTGGAAATGATTCAGATGAATTAGCAAATGGAGAAGTGGGAGGTGACCGGAATGAGAAGATGAAAAA TCAAATTAAGCAGATGATGGATGTCTCTCGGACACAGACTGCAATATCGGTAGTTGAAGAGGATCTAAAG CTTTTACAGCTTAAGCTAAGAGCCTCCGTGTCCACTAAATGTAACCTGGAAGACCAGGTAAAGAAATTGG AAGATGACCGCAACTCACTACAAGCTGCCAAAGCTGGACTGGAAGATGAATGCAAAACCTTGAGGCAGAA AGTGGAGATTCTGAATGAGCTCTATCAGCAGAAGGAGATGGCTTTGCAAAAGAAACTGAGTCAAGAAGAG TATGAACGGCAAGAAAGAGAGCACAGGCTGTCAGCTGCAGATGAAAAGGCAGTTTCGGCTGCAGAGGAAG TAAAAACTTACAAGCGGAGAATTGAAGAAATGGAGGATGAATTACAGAAGACAGAGCGGTCATTTAAAAA CCAGATCGCTACCCATGAGAAGAAAGCTCATGAAAACTGGCTCAAAGCTCGTGCTGCAGAAAGAGCTATA GCTGAAGAGAAAAGGGAAGCTGCCAATTTGAGACACAAATTATTAGAATTAACACAAAAGATGGCAATGC TGCAAGAAGAACCTGTGATTGTAAAACCAATGCCAGGAAAACCAAATACACAAAACCCTCCACGGAGAGG TCCTCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGAGAATGCTCCCCTCCATTGACA GTGGAGCCACCCGTGAGACCTCTCTCTGCTACTCTCAATCGAAGAGATATGCCTAGAAGTGAATTTGGAT CAGTGGACGGGCCTCTACCTCATCCTCGATGGTCAGCTGAGGCATCTGGGAAACCCTCTCCTTCTGATCC AGGATCTGGTACAGCTACCATGATGAACAGCAGCTCAAGAGGCTCTTCCCCTACCAGGGTACTCGATGAA GGCAAGGTTAATATGGCTCCAAAAGGGCCCCCTCCTTTCCCAGGAGTCCCTCTCATGAGCACCCCCATGG GAGGCCCTGTACCACCACCCATTCGATATGGACCACCACCTCAGCTCTGCGGACCTTTTGGGCCTCGGCC ACTTCCTCCACCCTTTGGCCCTGGTATGCGTCCACCACTAGGCTTAAGAGAATTTGCACCAGGCGTTCCA CCAGGAAGACGGGACCTGCCTCTCCACCCTCGGGGATTTTTACCTGGACACGCACCATTTAGACCTTTAG GTTCACTTGGCCCAAGAGAGTACTTTATTCCTGGTACCCGATTACCACCCCCAACCCATGGTCCCCAGGA ATACCCACCACCACCTGCTGTAAGAGACTTACTGCCGTCAGGCTCTAGAGATGAGCCTCCACCTGCCTCT CAGAGCACTAGCCAGGACTGTTCACAGGCTTTAAAACAGAGCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239559 representing NM_001300867 Red=Cloning site Green=Tags(s) MDSVPATVPSIAATPGDPELVGPLSVLYAAFIAKLLELVATLPDDVQPGPDFYGLPWKPVFITAFLGIAS FAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEA IKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIAL NEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNI NALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLK LLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEE YERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAI AEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLT VEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDE GKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVP PGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPAS QSTSQDCSQALKQSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300867
ORF Size	2355 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300867.1, NP_001287796.1
RefSeq Size	4924 bp
RefSeq ORF	2358 bp
Locus ID	375056
UniProt ID	Q5JRA6
MW	87.9 kDa
Gene Summary	Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255, PubMed:19269366). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed:28442536).[UniProtKB/Swiss-Prot Function]

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