Hsp105 (HSPH1) (NM_001286505) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HSP105; HSP105A; HSP105B; NY-CO-25
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239552 representing NM_001286505 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACGGCGGCCGTTCTCCGGGGACCGGCTGCCCATTGGGTAGAATCTTTCCAGAAGGCTCGAGAAG AAGGAAGCGGAAGTGGCACGTGGAGGGGCCGGTGGAGGCGCCGGTCAGTCATATCATTTGGATCAAAAAA TAGAACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTCAAA AGATTTCATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGATTTGG TTCCATTGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGTGTGGA GCAGATAACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCAGTAACA GATTGTGTTATTTCAGTACTGGGAACAGCTTTTGATCCTTTCTTAGGAGGAAAAAACTTCGATGAAAAGT TAGTGGAACATTTTTGTGCAGAATTTAAAACTAAGTACAAGTTGGATGCAAAATCCAAAATACGAGCACT CCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTAATGAGCTCTAACAGCACAGACCTTCCACTG AATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGAAAGATGAACAGGTCACAATTTGAAGAACTCT GTGCTGAACTTCTGCAAAAGATAGAAGTACCCCTTTATTCACTGTTGGAACAAACTCATCTCAAAGTAGA AGATGTGAGTGCAGTTGAGATTGTTGGAGGCGCTACACGAATTCCAGCTGTGAAGGAAAGAATTGCCAAA TTCTTTGGAAAAGATATTAGCACAACACTCAATGCAGATGAAGCAGTAGCCAGAGGATGTGCATTACAGT GTGCAATACTTTCCCCGGCATTTAAAGTTAGAGAATTTTCCGTCACAGATGCAGTTCCTTTTCCAATATC TCTGATCTGGAACCATGATTCAGAAGATACTGAAGGTGTTCATGAAGTCTTTAGTCGAAACCATGCTGCT CCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGGGGGCCTTTTGAGCTAGAAGCTTTCTATTCTGATCCCC AAGGAGTTCCATATCCAGAAGCAAAAATAGGCCGCTTTGTAGTTCAGAATGTTTCTGCACAGAAAGATGG AGAAAAATCTAGAGTAAAAGTCAAAGTGCGAGTCAACACCCATGGCATTTTCACCATCTCTACGGCATCT ATGGTGGAGAAAGTCCCAACTGAGGAGAATGAAATGTCTTCTGAAGCTGACATGGAGTGTCTGAATCAGA GACCACCAGAAAACCCAGACACTGATAAAAATGTCCAGCAAGACAACAGTGAAGCTGGAACACAGCCCCA GGTACAAACTGATGCTCAACAAACCTCACAGTCTCCCCCTTCACCTGAACTTACCTCAGAAGAAAACAAA ATCCCAGATGCTGACAAAGCAAATGAAAAAAAAGTTGACCAGCCTCCAGAAGCTAAAAAGCCCAAAATAA AGGTGGTGAATGTTGAGCTGCCTATTGAAGCCAACTTGGTCTGGCAGTTAGGGAAAGACCTTCTTAACAT GTATATTGAGACAGAGGGTAAGATGATAATGCAAGATAAATTGGAAAAAGAAAGGAATGATGCTAAAAAT GCAGTTGAGGAATATGTGTATGAGTTCAGAGACAAGCTGTGTGGACCATATGAAAAATTTATATGTGAGC AGGATCATCAAAATTTTTTGAGACTCCTCACAGAAACTGAAGACTGGCTGTATGAAGAAGGAGAGGACCA AGCTAAACAAGCATATGTTGACAAGTTGGAAGAATTAATGAAAATTGGCACTCCAGTTAAAGTTCGGTTT CAGGAAGCTGAAGAACGGCCAAAAATGTTTGAAGAACTAGGACAGAGGCTGCAGCATTATGCCAAGATAG CAGCTGACTTCAGAAATAAGGATGAGAAATACAACCATATTGATGAGTCTGAAATGAAAAAAGTGGAGAA GTCTGTTAATGAAGTGATGGAATGGATGAATAATGTCATGAATGCTCAGGCTAAAAAGAGTCTTGATCAG GATCCAGTTGTACGTGCTCAGGAAATTAAAACAAAAATCAAGGAATTGAACAACACATGTGAACCCGTTG TAACACAACCGAAACCAAAAATTGAATCACCCAAACTGGAAAGAACTCCAAATGGCCCAAATATTGATAA AAAGGAAGAAGATTTAGAAGACAAAAACAATTTTGGTGCTGAACCTCCACATCAGAATGGTGAATGTTAC CCTAATGAGAAAAATTCTGTTAATATGGACTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239552 representing NM_001286505 Red=Cloning site Green=Tags(s) MATAAVLRGPAAHWVESFQKAREEGSGSGTWRGRWRRRSVISFGSKNRTIGVAAKNQQITHANNTVSNFK RFHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKPVT DCVISVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKLMSSNSTDLPL NIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGATRIPAVKERIAK FFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTEGVHEVFSRNHAA PFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIFTISTAS MVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPELTSEENK IPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKERNDAKN AVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRF QEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQ DPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECY PNEKNSVNMDLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286505
ORF Size	2346 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286505.1, NP_001273434.1
RefSeq Size	4987 bp
RefSeq ORF	2349 bp
Locus ID	10808
UniProt ID	Q92598
Protein Families	Stem cell - Pluripotency
MW	89.3 kDa
Gene Summary	This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

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