SEMCAP3 (PDZRN3) (NM_001303139) Human Tagged ORF Clone
CAT#: RC239516
- TrueORF®
PDZRN3 (myc-DDK-tagged) - Human PDZ domain containing ring finger 3 (PDZRN3), transcript variant 2
ORF Plasmid: tGFP
"NM_001303139" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LNX3; SEMACAP3; SEMCAP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239516 representing NM_001303139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTAACCAGGTCAACGGCAGAGACTTATCCAGAGCAACTCATGACCAGGCTGTGGAAGCTTTCAAGA CAGCCAAGGAGCCCATAGTGGTGCAGGTGTTGAGAAGAACACCAAGGACCAAAATGTTCACGCCTCCATC AGAGTCTCAGCTGGTGGACACGGGAACCCAAACCGACATCACCTTTGAACATATCATGGCCCTCACTAAG ATGTCCTCTCCCAGCCCACCCGTGCTGGATCCCTATCTCTTGCCAGAGGAGCATCCCTCAGCCCATGAAT ACTACGATCCAAATGACTACATTGGAGACATCCATCAGGAGATGGACAGGGAGGAGCTGGAGCTGGAGGA AGTGGACCTCTACAGAATGAACAGCCAGGACAAGCTGGGCCTCACTGTGTGCTACCGGACGGACGATGAA GACGACATTGGGATTTATATCAGTGAGATTGACCCTAACAGCATTGCAGCCAAGGATGGGCGCATCCGAG AAGGAGACCGCATTATCCAGATTAATGGGATAGAGGTGCAGAACCGTGAAGAGGCTGTGGCTCTTCTAAC CAGTGAAGAAAATAAAAACTTTTCATTGCTGATTGCAAGGCCTGAACTCCAGCTGGATGAGGGCTGGATG GATGATGACAGGAACGACTTTCTGGATGACCTGCACATGGACATGCTGGAGGAGCAGCACCACCAGGCCA TGCAATTCACAGCTAGCGTGCTGCAGCAGAAGAAGCACGACGAAGACGGTGGGACCACAGATACAGCCAC CATCTTGTCCAACCAGCACGAGAAGGACAGCGGTGTGGGGCGGACCGACGAGAGCACCCGTAATGACGAG AGCTCGGAGCAAGAGAACAATGGCGACGACGCCACCGCATCCTCCAACCCGCTGGCGGGGCAGAGGAAGC TCACCTGCAGCCAGGACACCTTGGGCAGCGGCGACCTGCCCTTCAGCAACGAGTCTTTCATTTCGGCCGA CTGCACGGACGCCGACTACCTGGGGATCCCGGTGGACGAGTGCGAGCGCTTCCGCGAGCTCCTGGAGCTC AAGTGCCAGGTGAAGAGCGCCACCCCTTACGGCCTGTACTACCCTAGCGGCCCCCTGGACGCCGGCAAGA GTGACCCTGAGAGCGTGGACAAGGAGCTGGAGCTGCTGAACGAAGAGCTGCGCAGCATCGAGCTGGAGTG CCTGAGCATCGTGCGCGCCCACAAGATGCAGCAGCTCAAGGAGCAGTACCGCGAGTCCTGGATGCTGCAC AACAGCGGCTTCCGCAACTACAACACCAGCATCGACGTGCGCAGACACGAGCTCTCAGATATCACCGAGC TCCCGGAGAAATCCGACAAGGACAGCTCGAGCGCCTACAACACAGGCGAGAGCTGCCGCAGCACCCCGCT CACCCTGGAGATCTCCCCCGACAACTCCTTGAGGAGAGCGGCGGAGGGCATCAGCTGCCCGAGCAGCGAA GGGGCTGTGGGGACCACGGAAGCCTACGGGCCAGCCTCCAAGAATCTGCTCTCCATCACGGAAGATCCCG AAGTGGGCACCCCTACCTATAGCCCGTCCCTGAAGGAGCTGGACCCCAACCAGCCCCTGGAAAGCAAAGA GCGGAGAGCCAGCGACGGGAGCCGGAGCCCCACGCCCAGCCAGAAGCTGGGCAGCGCCTACCTGCCCTCC TATCACCACTCCCCATACAAGCACGCGCACATCCCGGCGCACGCCCAGCACTACCAGAGCTACATGCAGC TGATCCAGCAGAAGTCGGCCGTGGAGTACGCGCAAAGCCAGATGAGCCTGGTGAGCATGTGCAAGGACCT GAGCTCTCCCACCCCGTCGGAGCCGCGCATGGAGTGGAAGGTGAAGATCCGCAGCGACGGGACGCGCTAC ATCACCAAGAGGCCCGTGCGGGACCGCCTGCTGCGGGAGCGCGCCCTGAAGATCCGGGAAGAGCGCAGCG GCATGACCACCGACGACGACGCGGTGAGCGAGATGAAGATGGGGCGCTACTGGAGCAAGGAGGAGAGGAA GCAGCACCTGGTGAAGGCCAAGGAGCAGCGGCGGCGGCGCGAGTTCATGATGCAGAGCAGGTTGGATTGT CTCAAGGAGCAGCAAGCAGCCGATGACAGGAAGGAGATGAACATTCTCGAACTGAGCCACAAAAAGATGA TGAAGAAGAGGAATAAGAAAATCTTCGATAACTGGATGACGATCCAAGAACTCTTAACCCACGGCACAAA ATCCCCGGACGGCACTAGAGTATACAATTCCTTCCTATCGGTGACTACTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239516 representing NM_001303139
Red=Cloning site Green=Tags(s) MINQVNGRDLSRATHDQAVEAFKTAKEPIVVQVLRRTPRTKMFTPPSESQLVDTGTQTDITFEHIMALTK MSSPSPPVLDPYLLPEEHPSAHEYYDPNDYIGDIHQEMDREELELEEVDLYRMNSQDKLGLTVCYRTDDE DDIGIYISEIDPNSIAAKDGRIREGDRIIQINGIEVQNREEAVALLTSEENKNFSLLIARPELQLDEGWM DDDRNDFLDDLHMDMLEEQHHQAMQFTASVLQQKKHDEDGGTTDTATILSNQHEKDSGVGRTDESTRNDE SSEQENNGDDATASSNPLAGQRKLTCSQDTLGSGDLPFSNESFISADCTDADYLGIPVDECERFRELLEL KCQVKSATPYGLYYPSGPLDAGKSDPESVDKELELLNEELRSIELECLSIVRAHKMQQLKEQYRESWMLH NSGFRNYNTSIDVRRHELSDITELPEKSDKDSSSAYNTGESCRSTPLTLEISPDNSLRRAAEGISCPSSE GAVGTTEAYGPASKNLLSITEDPEVGTPTYSPSLKELDPNQPLESKERRASDGSRSPTPSQKLGSAYLPS YHHSPYKHAHIPAHAQHYQSYMQLIQQKSAVEYAQSQMSLVSMCKDLSSPTPSEPRMEWKVKIRSDGTRY ITKRPVRDRLLRERALKIREERSGMTTDDDAVSEMKMGRYWSKEERKQHLVKAKEQRRRREFMMQSRLDC LKEQQAADDRKEMNILELSHKKMMKKRNKKIFDNWMTIQELLTHGTKSPDGTRVYNSFLSVTTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001303139 |
ORF Size | 2292 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001303139.1, NP_001290068.1 |
RefSeq Size | 3595 bp |
RefSeq ORF | 2295 bp |
Locus ID | 23024 |
UniProt ID | Q9UPQ7 |
Protein Families | Druggable Genome |
MW | 87.4 kDa |
Gene Summary | This gene encodes a member of the LNX (Ligand of Numb Protein-X) family of RING-type ubiquitin E3 ligases. This protein may function in vascular morphogenesis and the differentiation of adipocytes, osteoblasts and myoblasts. This protein may be targeted for degradation by the human papilloma virus E6 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
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