SEMCAP3 (PDZRN3) (NM_001303139) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LNX3; SEMACAP3; SEMCAP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239516 representing NM_001303139 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTAACCAGGTCAACGGCAGAGACTTATCCAGAGCAACTCATGACCAGGCTGTGGAAGCTTTCAAGA CAGCCAAGGAGCCCATAGTGGTGCAGGTGTTGAGAAGAACACCAAGGACCAAAATGTTCACGCCTCCATC AGAGTCTCAGCTGGTGGACACGGGAACCCAAACCGACATCACCTTTGAACATATCATGGCCCTCACTAAG ATGTCCTCTCCCAGCCCACCCGTGCTGGATCCCTATCTCTTGCCAGAGGAGCATCCCTCAGCCCATGAAT ACTACGATCCAAATGACTACATTGGAGACATCCATCAGGAGATGGACAGGGAGGAGCTGGAGCTGGAGGA AGTGGACCTCTACAGAATGAACAGCCAGGACAAGCTGGGCCTCACTGTGTGCTACCGGACGGACGATGAA GACGACATTGGGATTTATATCAGTGAGATTGACCCTAACAGCATTGCAGCCAAGGATGGGCGCATCCGAG AAGGAGACCGCATTATCCAGATTAATGGGATAGAGGTGCAGAACCGTGAAGAGGCTGTGGCTCTTCTAAC CAGTGAAGAAAATAAAAACTTTTCATTGCTGATTGCAAGGCCTGAACTCCAGCTGGATGAGGGCTGGATG GATGATGACAGGAACGACTTTCTGGATGACCTGCACATGGACATGCTGGAGGAGCAGCACCACCAGGCCA TGCAATTCACAGCTAGCGTGCTGCAGCAGAAGAAGCACGACGAAGACGGTGGGACCACAGATACAGCCAC CATCTTGTCCAACCAGCACGAGAAGGACAGCGGTGTGGGGCGGACCGACGAGAGCACCCGTAATGACGAG AGCTCGGAGCAAGAGAACAATGGCGACGACGCCACCGCATCCTCCAACCCGCTGGCGGGGCAGAGGAAGC TCACCTGCAGCCAGGACACCTTGGGCAGCGGCGACCTGCCCTTCAGCAACGAGTCTTTCATTTCGGCCGA CTGCACGGACGCCGACTACCTGGGGATCCCGGTGGACGAGTGCGAGCGCTTCCGCGAGCTCCTGGAGCTC AAGTGCCAGGTGAAGAGCGCCACCCCTTACGGCCTGTACTACCCTAGCGGCCCCCTGGACGCCGGCAAGA GTGACCCTGAGAGCGTGGACAAGGAGCTGGAGCTGCTGAACGAAGAGCTGCGCAGCATCGAGCTGGAGTG CCTGAGCATCGTGCGCGCCCACAAGATGCAGCAGCTCAAGGAGCAGTACCGCGAGTCCTGGATGCTGCAC AACAGCGGCTTCCGCAACTACAACACCAGCATCGACGTGCGCAGACACGAGCTCTCAGATATCACCGAGC TCCCGGAGAAATCCGACAAGGACAGCTCGAGCGCCTACAACACAGGCGAGAGCTGCCGCAGCACCCCGCT CACCCTGGAGATCTCCCCCGACAACTCCTTGAGGAGAGCGGCGGAGGGCATCAGCTGCCCGAGCAGCGAA GGGGCTGTGGGGACCACGGAAGCCTACGGGCCAGCCTCCAAGAATCTGCTCTCCATCACGGAAGATCCCG AAGTGGGCACCCCTACCTATAGCCCGTCCCTGAAGGAGCTGGACCCCAACCAGCCCCTGGAAAGCAAAGA GCGGAGAGCCAGCGACGGGAGCCGGAGCCCCACGCCCAGCCAGAAGCTGGGCAGCGCCTACCTGCCCTCC TATCACCACTCCCCATACAAGCACGCGCACATCCCGGCGCACGCCCAGCACTACCAGAGCTACATGCAGC TGATCCAGCAGAAGTCGGCCGTGGAGTACGCGCAAAGCCAGATGAGCCTGGTGAGCATGTGCAAGGACCT GAGCTCTCCCACCCCGTCGGAGCCGCGCATGGAGTGGAAGGTGAAGATCCGCAGCGACGGGACGCGCTAC ATCACCAAGAGGCCCGTGCGGGACCGCCTGCTGCGGGAGCGCGCCCTGAAGATCCGGGAAGAGCGCAGCG GCATGACCACCGACGACGACGCGGTGAGCGAGATGAAGATGGGGCGCTACTGGAGCAAGGAGGAGAGGAA GCAGCACCTGGTGAAGGCCAAGGAGCAGCGGCGGCGGCGCGAGTTCATGATGCAGAGCAGGTTGGATTGT CTCAAGGAGCAGCAAGCAGCCGATGACAGGAAGGAGATGAACATTCTCGAACTGAGCCACAAAAAGATGA TGAAGAAGAGGAATAAGAAAATCTTCGATAACTGGATGACGATCCAAGAACTCTTAACCCACGGCACAAA ATCCCCGGACGGCACTAGAGTATACAATTCCTTCCTATCGGTGACTACTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239516 representing NM_001303139 Red=Cloning site Green=Tags(s) MINQVNGRDLSRATHDQAVEAFKTAKEPIVVQVLRRTPRTKMFTPPSESQLVDTGTQTDITFEHIMALTK MSSPSPPVLDPYLLPEEHPSAHEYYDPNDYIGDIHQEMDREELELEEVDLYRMNSQDKLGLTVCYRTDDE DDIGIYISEIDPNSIAAKDGRIREGDRIIQINGIEVQNREEAVALLTSEENKNFSLLIARPELQLDEGWM DDDRNDFLDDLHMDMLEEQHHQAMQFTASVLQQKKHDEDGGTTDTATILSNQHEKDSGVGRTDESTRNDE SSEQENNGDDATASSNPLAGQRKLTCSQDTLGSGDLPFSNESFISADCTDADYLGIPVDECERFRELLEL KCQVKSATPYGLYYPSGPLDAGKSDPESVDKELELLNEELRSIELECLSIVRAHKMQQLKEQYRESWMLH NSGFRNYNTSIDVRRHELSDITELPEKSDKDSSSAYNTGESCRSTPLTLEISPDNSLRRAAEGISCPSSE GAVGTTEAYGPASKNLLSITEDPEVGTPTYSPSLKELDPNQPLESKERRASDGSRSPTPSQKLGSAYLPS YHHSPYKHAHIPAHAQHYQSYMQLIQQKSAVEYAQSQMSLVSMCKDLSSPTPSEPRMEWKVKIRSDGTRY ITKRPVRDRLLRERALKIREERSGMTTDDDAVSEMKMGRYWSKEERKQHLVKAKEQRRRREFMMQSRLDC LKEQQAADDRKEMNILELSHKKMMKKRNKKIFDNWMTIQELLTHGTKSPDGTRVYNSFLSVTTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303139
ORF Size	2292 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303139.1, NP_001290068.1
RefSeq Size	3595 bp
RefSeq ORF	2295 bp
Locus ID	23024
UniProt ID	Q9UPQ7
Protein Families	Druggable Genome
MW	87.4 kDa
Gene Summary	This gene encodes a member of the LNX (Ligand of Numb Protein-X) family of RING-type ubiquitin E3 ligases. This protein may function in vascular morphogenesis and the differentiation of adipocytes, osteoblasts and myoblasts. This protein may be targeted for degradation by the human papilloma virus E6 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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