SP140 (NM_001278453) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LYSP100; LYSP100-A; LYSP100-B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239485 representing NM_001278453 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCAGGGCCAGCAGGGGCAGATGGCAAGTGGAGACAGCAATCTCAACTTCAGGATGGTCGCAG AGATCCAGAACGTAGAGGGTCAGAACCTGCAGGAGCAGGTTTGCCCTGAGCCCATTTTCAGGTTCTTCAG AGAAAACAAGGTGGAGATTGCAAGTGCAATAACAAGGCCATTTCCTTTCCTTATGGGCCTCCGAGACCGC TCCTTCATCTCCGAGCAGATGTATGAACATTTTCAAGAAGCTTTTAGAAACCTGGTCCCAGTGACAAGAG TGATGTATTGTGTACTCAGTGAACTGGAGAAGACATTTGGCTGGTCACATCTGGAAGCATTGTTCAGCAG GATTAACCTGATGGCCTATCCTGATTTAAACGAGATTTACAGAAGCTTCCAGAATGTATGCTATGAACAC TCACCTCTCCAAATGAATAATGTAAACGATTTAGAAGATAGACCCAGATTACTACCATATGGTAAACAAG AGAACAGCAATGCCTGTCATGAAATGGATGATATAGCAGTGCCTCAGGAAGCCTTGAGCTCCTCGCCAAG GTGTGAGCCAGGTTTCTCTTCAGAGTCTTGTGAGCAGTTAGCTCTCCCAAAGGCTGGTGGAGGAGATGCT GAAGATGCACCCAGCCTACTACCAGTGTCCTGTAAACTTGCTATACAAATAGATGAAGGAGAATCAGAAG AAATGCCCAAGTTACTGCCTTATGATACAGAAGAGACCTTTGATCTAAAAACTCCCCAAGTCACTAATGA AGGAGAACCAGAGAAGGGGCTCTGTCTACTACCAGGTGAAGGAGAAGAGGGCAGTGATGACTGTTCGGAA ATGTGTGATGGAGAAGAGCGCCAGGAAGCCTCTAGCTCCCTAGCAAGACGTGGGTCAGTGTCTAGTGAAC TAGAAAATCACCCAATGAATGAAGAAGGAGAATCAGAAGAGCTTGCTTCTAGCCTGCTATATGATAATGT ACCAGGAGCGGAGCAATCAGCATATGAAAATGAGAAGTGTTCCTGTGTCATGTGTTTCTCAGAAGAGGTG CCAGGAAGCCCAGAAGCAAGGACGGAAAGTGATCAAGCGTGTGGCACAATGGATACTGTGGATATTGCAA ACAACTCCACTTTGGGAAAACCCAAGAGGAAAAGAAGAAAAAAGAGGGGGCATGGCTGGAGCAGAATGAG AATGAGAAGGCAGGAAAACAGCCAACAAAATGATAATAGCAAAGCCGACGGCCAGGTGGTCTCCAGTGAA AAGAAGGCGAACGTGAATCTGAAAGACCTTTCCAAGATTAGGGGGAGAAAGAGAGGCAAACCTGGAACCC GCTTCACTCAGAGTGACAGAGCTGCACAGAAAAGAGTCCGATCAAGAGCTTCAAGAAAGCACAAAGATGA AACTGTGGATTTTAAGGCTCCTTTGCTTCCAGTGACCTGTGGTGGGGTGAAGGGAATTTTACATAAGAAG AAATTGCAGCAAGGAATCTTGGTGAAGTGTATACAGACTGAGGATGGAAAATGGTTCACCCCCACGGAAT TTGAAATCAAAGGAGGCCATGCAAGATCAAAGAACTGGAGGCTGAGTGTGCGCTGTGGCGGGTGGCCCCT ACGATGGCTGATGGAGAATGGATTTCTGCCTGATCCTCCAAGAATACGTTACAGGAAAAAAAAGAGAATA CTGAAGTCTCAAAACAATAGCTCAGTTGACCCTTGTATGAGAAACCTGGATGAGTGTGAGGTGTGCCGGG ACGGAGGGGAGCTGTTCTGTTGCGACACTTGTTCAAGAGTCTTCCATGAGGACTGTCACATCCCGCCTGT GGAAGCTGAGAGGACCCCGTGGAATTGCATCTTCTGCAGGATGAAGGAGTCTCCGGGAAGCCAACAGTGT TGTCAGGAATCTGAGGTCCTGGAGAGGCAGATGTGTCCTGAGGAACAGTTGAAATGTGAGTTCCTCCTCT TGAAAGTCTATTGCTGTTCTGAGAGCTCCTTTTTTGCCAAGATTCCATACTATTATTATATTAGAGAGGC GTGTCAAGGCCTGAAGGAGCCCATGTGGTTGGATAAAATCAAGAAAAGGCTGAATGAGCACGGTTACCCC CAAGTGGAGGGGTTTGTACAAGACATGCGCCTCATCTTCCAGAACCACAGGGCCTCTTACAAGTACAAGG ATTTTGGCCAAATGGGATTTAGACTGGAGGCTGAGTTTGAGAAGAATTTCAAGGAAGTGTTTGCTATTCA GGAAACAAATGGGAACAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239485 representing NM_001278453 Red=Cloning site Green=Tags(s) MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRPFPFLMGLRDR SFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINLMAYPDLNEIYRSFQNVCYEH SPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQEALSSSPRCEPGFSSESCEQLALPKAGGGDA EDAPSLLPVSCKLAIQIDEGESEEMPKLLPYDTEETFDLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSE MCDGEERQEASSSLARRGSVSSELENHPMNEEGESEELASSLLYDNVPGAEQSAYENEKCSCVMCFSEEV PGSPEARTESDQACGTMDTVDIANNSTLGKPKRKRRKKRGHGWSRMRMRRQENSQQNDNSKADGQVVSSE KKANVNLKDLSKIRGRKRGKPGTRFTQSDRAAQKRVRSRASRKHKDETVDFKAPLLPVTCGGVKGILHKK KLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWLMENGFLPDPPRIRYRKKKRI LKSQNNSSVDPCMRNLDECEVCRDGGELFCCDTCSRVFHEDCHIPPVEAERTPWNCIFCRMKESPGSQQC CQESEVLERQMCPEEQLKCEFLLLKVYCCSESSFFAKIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYP QVEGFVQDMRLIFQNHRASYKYKDFGQMGFRLEAEFEKNFKEVFAIQETNGNN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278453
ORF Size	2259 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278453.2
RefSeq Size	2928 bp
RefSeq ORF	2262 bp
Locus ID	11262
UniProt ID	Q13342
Protein Families	Druggable Genome, Transcription Factors
MW	86.5 kDa
Gene Summary	This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016]

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