MTSS1 (NM_001282974) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MIM; MIMA; MIMB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239432 representing NM_001282974 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGTGATTGAGAAGGAATGCAGCGCGCTCGGAGGCCTCTTCCAGACCATCATCAGCGACATGA AGGGGAGCTATCCAGTTTGGGAAGATTTCATAAACAAAGCAGGAAAGCTGCAGTCCCAGCTTCGGACAAC AGTAGTAGCAGCAGCTGCCTTCTTGGACGCCTTTCAGAAAGTGGCTGACATGGCCACCAACACACGTGGT GGGACCAGGGAGATTGGATCTGCTCTCACCAGGATGTGCATGAGGCACAGAAGCATTGAAGCCAAGCTGA GGCAGTTTTCGAGCGCTTTAATTGATTGTCTGATAAACCCACTTCAAGAACAGATGGAAGAATGGAAGAA AGTGGCCAACCAGCTGGATAAAGACCACGCAAAAGAATATAAGAAAGCCCGCCAAGAGATAAAAAAGAAG TCCTCGGATACGCTGAAACTGCAGAAGAAAGCAAAAAAAGGGAGAGGTGATATCCAGCCTCAGTTGGACA GTGCTCTCCAAGATGTCAATGATAAGTATCTCTTATTGGAAGAAACAGAAAAGCAGGCTGTCCGGAAGGC TTTGATTGAAGAACGTGGCCGATTCTGTACCTTCATCTCTATGCTGCGGCCAGTGATTGAAGAAGAAATC TCAATGCTAGGGGAAATAACCCACCTTCAGACCATCTCGGAAGATCTAAAAAGCCTGACCATGGACCCTC ACAAACTGCCCTCCTCAAGTGAACAGGTGATTCTGGACTTGAAAGGTTCTGATTACAGCTGGTCGTATCA GACGCCACCCTCTTCCCCCAGCACCACCATGTCCAGAAAGTCCAGTGTCTGCAGCAGCCTGAACAGTGTC AACAGCAGTGACTCCCGGTCCAGCGGCTCCCACTCGCATTCCCCCAGCTCACATTACCGCTACCGCAGCT CCAACCTGGCCCAGCAGGCTCCTGTGAGGCTGTCCAGCGTGTCCTCCCATGACTCAGGATTCATATCCCA GGATGCCTTCCAGTCCAAGTCACCATCCCCCATGCCGCCAGAGGCCCCCAACCAGAACTCGTCCAGCTCG GCCTCCTCCGAAGCCTCGGAAACCTGCCAGTCAGTGAGCGAGTGCAGCTCCCCCACCTCTGTCAGCTCGG GCTCCACCATGGGTGCCTGGGTGTCCACAGAGAAGGACTGGGCTAAGCCTGGGCCCTATGACCAGCCTCT GGTGAACACCCTGCAGCGCCGCAAAGAGAAGCGAGAACCGGACCCCAACGGGGGAGGACCCACTACCGCC AGCGGCCCACCTGCAGCAGCTGAGGAGGCTCAGAGACCACGGAGCATGACTGTATCGGCTGCCACCAGGC CTGGTGAGGAGATGGAGGCTTGTGAGGAGCTGGCCCTGGCCCTGTCTCGGGGCCTGCAGCTGGACACCCA GAGGAGCAGCCGGGACTCGCTTCAGTGCTCCAGCGGCTACAGCACCCAGACAACCACCCCCTGCTGCTCT GAGGACACCATCCCTTCCCAAGTTTCAGATTATGATTATTTCTCTGTAAGTGGTGACCAGGAGGCAGATC AGCAGGAGTTCGACAAGTCCTCCACCATTCCAAGAAACAGCGACATCAGCCAGTCCTACCGACGGATGTT CCAAGCCAAGCGTCCAGCCTCAACTGCTGGCCTCCCCACCACCCTGGGACCTGCTATGGTCACTCCAGGG GTTGCAACTATCCGACGGACCCCTTCCACCAAGCCTTCTGTCCGCCGGGGAACCATTGGAGCTGGTCCCA TCCCCATCAAGACACCCGTGATCCCTGTCAAGACCCCAACCGTCCCAGACCTCCCAGGGGTGTTGCCAGC CCCTCCAGATGGGCCAGAAGAGCGGGGGGAGCACAGCCCTGAGTCGCCATCTGTGGGTGAGGGCCCCCAA GGTGTCACCAGCATGCCCTCCTCAATGTGGAGCGGCCAAGCTTCCGTTAACCCTCCACTTCCAGGCCCGA AGCCCAGTATCCCTGAGGAGCACAGACAGGCAATTCCAGAAAGTGAAGCTGAAGACCAGGAACGGGAACC CCCAAGTGCCACTGTCTCCCCAGGCCAGATTCCAGAGAGTGACCCTGCAGACCTGAGCCCAAGGGATACT CCACAAGGAGAAGACATGCTGAACGCCATCCGAAGGGGCGTGAAACTGAAGAAGACCACGACAAACGATC GCTCAGCCCCTCGCTTTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239432 representing NM_001282974 Red=Cloning site Green=Tags(s) MEAVIEKECSALGGLFQTIISDMKGSYPVWEDFINKAGKLQSQLRTTVVAAAAFLDAFQKVADMATNTRG GTREIGSALTRMCMRHRSIEAKLRQFSSALIDCLINPLQEQMEEWKKVANQLDKDHAKEYKKARQEIKKK SSDTLKLQKKAKKGRGDIQPQLDSALQDVNDKYLLLEETEKQAVRKALIEERGRFCTFISMLRPVIEEEI SMLGEITHLQTISEDLKSLTMDPHKLPSSSEQVILDLKGSDYSWSYQTPPSSPSTTMSRKSSVCSSLNSV NSSDSRSSGSHSHSPSSHYRYRSSNLAQQAPVRLSSVSSHDSGFISQDAFQSKSPSPMPPEAPNQNSSSS ASSEASETCQSVSECSSPTSVSSGSTMGAWVSTEKDWAKPGPYDQPLVNTLQRRKEKREPDPNGGGPTTA SGPPAAAEEAQRPRSMTVSAATRPGEEMEACEELALALSRGLQLDTQRSSRDSLQCSSGYSTQTTTPCCS EDTIPSQVSDYDYFSVSGDQEADQQEFDKSSTIPRNSDISQSYRRMFQAKRPASTAGLPTTLGPAMVTPG VATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDLPGVLPAPPDGPEERGEHSPESPSVGEGPQ GVTSMPSSMWSGQASVNPPLPGPKPSIPEEHRQAIPESEAEDQEREPPSATVSPGQIPESDPADLSPRDT PQGEDMLNAIRRGVKLKKTTTNDRSAPRFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282974
ORF Size	2190 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282974.1, NP_001269903.1
RefSeq Size	4979 bp
RefSeq ORF	2193 bp
Locus ID	9788
UniProt ID	O43312
Protein Families	Druggable Genome
MW	79.6 kDa
Gene Summary	May be related to cancer progression or tumor metastasis in a variety of organ sites, most likely through an interaction with the actin cytoskeleton.[UniProtKB/Swiss-Prot Function]

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