LARGE2 (NM_001300721) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GYLTL1B; PP5656
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239412 representing NM_001300721 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCGAGGGCGCCCCCGGGCGCTGGGGGCCGCCGCGCTGTTGCTGCTGCTGCTGCTGCTCGGAT TCCTCCTGTTCGGTGGGGACCTGGGGTGTGAGCGCCGCAAGCCTGGCGGGCGAGCGGGGGCCCCGGGATG CTTCCCCGGCCCGCTCATGCCACGTGTCCCCCCAGACGGGAGGCTGCGGAGAGCCGCCGCCCTCGACGGA GACCCGGGGGCCGGCCCCGGGGACCACAACCGCTCCGACTGCGGCCCGCAGCCGCCGCCGCCGCCCAAGT GCGAGCTCTTGCATGTGGCCATCGTGTGTGCGGGGCATAACTCCAGCCGAGACGTCATCACCCTGGTGAA GTCCATGCTCTTCTACAGGAAAAATCCACTGCACCTCCACTTGGTGACTGACGCCGTGGCCAGAAACATC CTGGAGACGCTCTTCCACACATGGATGGTGCCTGCTGTCCGTGTCAGCTTTTATCATGCCGACCAGCTCA AGCCCCAGGTCTCCTGGATCCCCAACAAGCACTACTCCGGCCTCTATGGGCTAATGAAGCTGGTGCTGCC CAGTGCCTTGCCTGCTGAGCTGGCCCGCGTCATTGTCCTGGACACGGATGTCACCTTCGCCTCTGACATC TCGGAGCTCTGGGCCCTCTTTGCTCACTTTTCTGACACGCAGGCGATCGGTCTTGTGGAGAACCAGAGTG ACTGGTACCTGGGCAACCTCTGGAAGAACCACAGGCCCTGGCCTGCCTTGGGCCGGGGATTTAACACAGG TGTGATCCTGCTGCGGCTGGACCGGCTCCGGCAGGCTGGCTGGGAGCAGATGTGGAGGCTGACAGCCAGG CGGGAGCTCCTTAGCCTGCCTGCCACCTCACTGGCTGACCAGGACATCTTCAACGCTGTGATCAAGGAGC ACCCGGGGCTAGTGCAGCGTCTGCCTTGTGTCTGGAATGTGCAGCTGTCAGATCACACACTGGCCGAGCG CTGCTACTCTGAGGCGTCTGACCTCAAGGTGATCCACTGGAACTCACCAAAGAAGCTTCGGGTGAAGAAC AAGCATGTGGAATTCTTCCGCAATTTCTACCTGACCTTCCTGGAGTACGATGGGAACCTGCTGCGGAGAG AGCTCTTTGTGTGCCCCAGCCAGCCCCCACCTGGTGCTGAGCAGTTGCAGCAGGCCCTGGCACAACTGGA CGAGGAAGACCCCTGCTTTGAGTTCCGGCAGCAGCAGCTCACTGTGCACCGTGTGCATGTCACTTTCCTG CCCCATGAACCGCCACCCCCCCGGCCTCACGATGTCACCCTTGTGGCCCAGCTGTCCATGGACCGGCTGC AGATGTTGGAAGCCCTGTGCAGGCACTGGCCTGGCCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGA AGCTCAGCAGTTCCTGCATTTCGTCGAGGCCTCACCAGTGCTTGCTGCCCGGCAGGACGTGGCCTACCAT GTGGTGTACCGTGAGGGGCCCCTATACCCCGTCAACCAGCTTCGCAACGTGGCCTTGGCCCAGGCCCTCA CGCCTTACGTCTTCCTCAGTGACATTGACTTCCTGCCTGCCTATTCTCTCTACGACTACCTCAGGGCCTC CATTGAGCAGCTGGGGCTGGGCAGCCGGCGCAAGGCAGCACTGGTGGTGCCGGCATTTGAGACCCTGCGC TACCGCTTCAGCTTCCCCCATTCCAAGGTGGAGCTGTTGGCCTTGCTGGATGCGGGCACTCTCTACACCT TCAGGTACCACGAGTGGCCCCGAGGCCACGCACCCACAGACTATGCCCGCTGGCGGGAGGCTCAGGCCCC GTACCGTGTGCAATGGGCGGCCAACTATGAACCCTACGTGGTGGTGCCACGAGACTGTCCCCGCTATGAT CCTCGCTTTGTGGGCTTCGGCTGGAACAAAGTGGCCCACATTGTGGAGCTGGATGCCCAGGAATATGAGC TCCTGGTGCTGCCCGAGGCCTTCACCATCCATCTGCCCCACGCTCCAAGCCTGGACATCTCCCGCTTCCG CTCCAGCCCCACCTATCGTGACTGCCTCCAGGCCCTCAAGGACGAATTCCACCAGGACTTGTCCCGCCAC CATGGGGCTGCTGCCCTCAAATACCTCCCAGCCCTGCAGCAGCCCCAGAGCCCTGCCCGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239412 representing NM_001300721 Red=Cloning site Green=Tags(s) MLPRGRPRALGAAALLLLLLLLGFLLFGGDLGCERRKPGGRAGAPGCFPGPLMPRVPPDGRLRRAAALDG DPGAGPGDHNRSDCGPQPPPPPKCELLHVAIVCAGHNSSRDVITLVKSMLFYRKNPLHLHLVTDAVARNI LETLFHTWMVPAVRVSFYHADQLKPQVSWIPNKHYSGLYGLMKLVLPSALPAELARVIVLDTDVTFASDI SELWALFAHFSDTQAIGLVENQSDWYLGNLWKNHRPWPALGRGFNTGVILLRLDRLRQAGWEQMWRLTAR RELLSLPATSLADQDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKN KHVEFFRNFYLTFLEYDGNLLRRELFVCPSQPPPGAEQLQQALAQLDEEDPCFEFRQQQLTVHRVHVTFL PHEPPPPRPHDVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEASPVLAARQDVAYH VVYREGPLYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLRASIEQLGLGSRRKAALVVPAFETLR YRFSFPHSKVELLALLDAGTLYTFRYHEWPRGHAPTDYARWREAQAPYRVQWAANYEPYVVVPRDCPRYD PRFVGFGWNKVAHIVELDAQEYELLVLPEAFTIHLPHAPSLDISRFRSSPTYRDCLQALKDEFHQDLSRH HGAAALKYLPALQQPQSPARG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300721
ORF Size	2163 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300721.1, NP_001287650.1
RefSeq Size	2546 bp
RefSeq ORF	2166 bp
Locus ID	120071
UniProt ID	Q8N3Y3
Protein Families	Transmembrane
MW	81.8 kDa
Gene Summary	Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function]

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