CDT2 (DTL) (NM_001286230) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDT2; DCAF2; L2DTL; RAMP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239313 representing NM_001286230 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTCAATTCGGTGCTCCGCCAGCCCCAGCTTGGCGTCCTGAGAAATGCTCCCAATATGGAACATG TACTAGCAGTTGCCAATGAAGAAGGCTTTGTTCGATTGTATAACACAGAATCACAAAGTTTCAGAAAGAA GTGCTTCAAAGAATGGATGGCTCACTGGAATGCCGTCTTTGACCTGGCCTGGGTTCCTGGTGAACTTAAA CTTGTTACAGCAGCAGGTGATCAAACAGCCAAATTTTGGGACGTAAAAGCTGGTGAGCTGATTGGAACAT GCAAAGGTCATCAATGCAGCCTCAAGTCAGTTGCCTTTTCTAAGTTTGAGAAAGCTGTATTCTGTACGGG TGGAAGAGATGGCAACATTATGGTCTGGGATACCAGGTGCAACAAAAAAGATGGGTTTTATAGGCAAGTG AATCAAATCAGTGGAGCTCACAATACCTCAGACAAGCAAACCCCTTCAAAACCCAAGAAGAAACAGAATT CAAAAGGACTTGCTCCTTCTGTGGATTTCCAGCAAAGTGTTACTGTGGTCCTCTTTCAAGACGAGAATAC CTTAGTCTCAGCAGGAGCTGTGGATGGGATAATCAAAGTATGGGATTTACGTAAGAATTATACTGCTTAT CGACAAGAACCCATAGCATCCAAGTCTTTCCTGTACCCAGGTAGCAGCACTCGAAAACTTGGATATTCAA GTCTGATTTTGGATTCCACTGGCTCTACTTTATTTGCTAATTGCACAGACGATAACATCTACATGTTTAA TATGACTGGGTTGAAGACTTCTCCAGTGGCTATTTTCAATGGACACCAGAACTCTACCTTTTATGTAAAA TCCAGCCTTAGTCCAGATGACCAGTTTTTAGTCAGTGGCTCAAGTGATGAAGCTGCCTACATATGGAAGG TCTCCACACCCTGGCAACCTCCTACTGTGCTCCTGGGTCATTCTCAAGAGGTCACGTCTGTGTGCTGGTG TCCATCTGACTTCACAAAGATTGCTACCTGTTCTGATGACAATACACTAAAAATCTGGCGCTTGAATAGA GGCTTAGAGGAGAAACCAGGAGGTGATAAACTTTCCACGGTGGGTTGGGCCTCTCAGAAGAAAAAAGAGT CAAGACCTGGCCTAGTAACAGTAACGAGTAGCCAGAGTACTCCTGCCAAAGCCCCCAGGGTAAAGTGCAA TCCATCCAATTCTTCCCCGTCATCCGCAGCTTGTGCCCCAAGCTGTGCTGGAGACCTCCCTCTTCCTTCA AATACTCCTACGTTCTCTATTAAAACCTCTCCTGCCAAGGCCCGGTCTCCCATCAACAGAAGAGGCTCTG TCTCCTCCGTCTCTCCCAAGCCACCTTCATCTTTCAAGATGTCGATTAGAAACTGGGTGACCCGAACACC TTCCTCATCACCACCCATCACTCCACCTGCTTCGGAGACCAAGATCATGTCTCCGAGAAAAGCCCTTATT CCTGTGAGCCAGAAGTCATCCCAAGCAGAGGCTTGCTCTGAGTCTAGAAATAGAGTAAAGAGGAGGCTAG ACTCAAGCTGTCTGGAGAGTGTGAAACAAAAGTGTGTGAAGAGTTGTAACTGTGTGACTGAGCTTGATGG CCAAGTTGAAAATCTTCATTTGGATCTGTGCTGCCTTGCTGGTAACCAGGAAGACCTTAGTAAGGACTCT CTAGGTCCTACCAAATCAAGCAAAATTGAAGGAGCTGGTACCAGTATCTCAGAGCCTCCGTCTCCTATCA GTCCGTATGCTTCAGAAAGCTGTGGAACGCTACCTCTTCCTTTGAGACCTTGTGGAGAAGGGTCTGAAAT GGTAGGCAAAGAGAATAGTTCCCCAGAGAATAAAAACTGGTTGTTGGCCATGGCAGCCAAACGGAAGGCT GAGAATCCATCTCCACGAAGTCCGTCATCCCAGACACCCAATTCCAGGAGACAGAGCGGAAAGACATTGC CAAGCCCGGTCACCATCACGCCCAGCTCCATGAGGAAAATCTGCACATACTTCCATAGAAAGTCCCAGGA GGACTTCTGTGGTCCTGAACACTCAACAGAATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239313 representing NM_001286230 Red=Cloning site Green=Tags(s) MLFNSVLRQPQLGVLRNAPNMEHVLAVANEEGFVRLYNTESQSFRKKCFKEWMAHWNAVFDLAWVPGELK LVTAAGDQTAKFWDVKAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQV NQISGAHNTSDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGIIKVWDLRKNYTAY RQEPIASKSFLYPGSSTRKLGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAIFNGHQNSTFYVK SSLSPDDQFLVSGSSDEAAYIWKVSTPWQPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKIWRLNR GLEEKPGGDKLSTVGWASQKKKESRPGLVTVTSSQSTPAKAPRVKCNPSNSSPSSAACAPSCAGDLPLPS NTPTFSIKTSPAKARSPINRRGSVSSVSPKPPSSFKMSIRNWVTRTPSSSPPITPPASETKIMSPRKALI PVSQKSSQAEACSESRNRVKRRLDSSCLESVKQKCVKSCNCVTELDGQVENLHLDLCCLAGNQEDLSKDS LGPTKSSKIEGAGTSISEPPSPISPYASESCGTLPLPLRPCGEGSEMVGKENSSPENKNWLLAMAAKRKA ENPSPRSPSSQTPNSRRQSGKTLPSPVTITPSSMRKICTYFHRKSQEDFCGPEHSTEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286230
ORF Size	2064 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286230.1, NP_001273159.1
RefSeq Size	4485 bp
RefSeq ORF	2067 bp
Locus ID	51514
Protein Families	Druggable Genome
MW	75.5 kDa
Gene Summary	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613, PubMed:27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.