FIGNL1 (NM_001287492) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239283 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACCTCCAGCTCTAGATCTGTGCACCTGAGTGAATGGCAGAAGAATTACTTCGCAATTACATCTG GCATATGTACCGGACCGAAGGCAGATGCATACCGTGCACAGATATTACGCATTCAGTATGCATGGGCAAA CTCTGAGATTTCCCAGGTCTGTGCTACCAAACTGTTCAAAAAATATGCAGAGAAATATTCTGCAATTATT GATTCTGACAATGTTGAATCTGGGTTGAATAATTATGCAGAAAACATTTTAACTTTGGCAGGATCTCAAC AAACAGATAGTGACAAGTGGCAGTCTGGATTGTCAATAAATAATGTTTTCAAAATGAGTAGTGTACAGAA GATGATGCAAGCTGGCAAAAAATTCAAAGACTCTCTGTTGGAACCTGCTCTTGCATCAGTGGTAATCCAT AAGGAGGCCACTGTCTTTGATCTTCCTAAATTTAGTGTTTGTGGTAGTTCTCAAGAGAGTGACTCATTAC CTAACTCAGCTCATGATCGAGACCGGACCCAAGACTTCCCGGAGAGCAATCGTTTGAAACTCCTTCAGAA TGCCCAGCCACCTATGGTGACTAACACTGCTAGGACTTGTCCTACATTCTCAGCACCTGTAGGTGAGTCA GCTACTGCAAAATTCCATGTCACACCATTGTTTGGAAATGTCAAAAAGGAAAATCACAGCTCTGCAAAAG AAAACATAGGACTTAATGTGTTCTTATCTAACCAGTCTTGTTTTCCTGCTGCCTGTGAAAATCCACAGAG GAAGTCTTTTTATGGTTCTGGCACCATTGATGCACTTTCCAATCCAATACTGAATAAGGCTTGTAGTAAA ACAGAAGATAATGGCCCAAAGGAGGATAGCAGCCTGCCTACATTTAAAACTGCAAAAGAACAATTATGGG TAGATCAGCAAAAAAAGTACCACCAACCTCAGCGTGCATCAGGGTCTTCATATGGTGGTGTAAAAAAGTC TCTAGGAGCTAGTAGATCCCGAGGGATACTTGGAAAGTTTGTTCCTCCTATACCCAAGCAAGATGGGGGA GAGCAGAATGGAGGAATGCAATGTAAGCCTTATGGGGCAGGACCTACAGAACCAGCACATCCAGTTGATG AGCGTCTGAAGAACTTGGAGCCAAAGATGATTGAACTTATTATGAATGAGATTATGGATCATGGACCTCC AGTAAATTGGGAAGATATTGCAGGAGTAGAATTTGCTAAAGCCACCATAAAGGAAATAGTTGTGTGGCCC ATGTTGAGGCCAGACATCTTTACTGGTTTAAGGGGACCCCCTAAAGGAATTTTGCTCTTTGGTCCTCCTG GGACTGGTAAAACTCTAATTGGCAAGTGCATTGCTAGTCAGTCTGGGGCAACATTCTTTAGCATCTCTGC TTCATCCTTAACTTCTAAATGGGTAGGTGAGGGGGAGAAAATGGTCCGTGCATTGTTTGCTGTTGCAAGG TGTCAGCAACCAGCTGTGATATTTATTGACGAAATTGATTCCTTGTTATCTCAACGGGGAGATGGTGAGC ATGAATCTTCTAGAAGGATAAAAACAGAATTTTTAGTTCAATTAGATGGAGCAACAACATCTTCTGAAGA TCGTATCCTAGTGGTGGGAGCAACAAATCGGCCACAAGAAATTGATGAGGCTGCCCGGAGAAGATTGGTG AAAAGGCTTTATATTCCCCTCCCAGAAGCTTCAGCCAGGAAACAGATAGTAATTAATCTAATGTCCAAAG AGCAGTGTTGCCTCAGTGAAGAAGAAATTGAACAGATTGTACAGCAGTCTGATGCGTTTTCAGGAGCAGA CATGACACAGCTTTGCAGGGAGGCTTCTCTTGGTCCTATTCGCAGTTTACAAACTGCTGACATTGCTACC ATAACACCGGATCAAGTTCGACCCATAGCTTACATTGATTTTGAAAATGCTTTTAGAACTGTGCGACCTA GTGTTTCTCCAAAAGATTTAGAGCTTTATGAAAACTGGAACAAAACTTTTGGTTGTGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239283 protein sequence Red=Cloning site Green=Tags(s) MQTSSSRSVHLSEWQKNYFAITSGICTGPKADAYRAQILRIQYAWANSEISQVCATKLFKKYAEKYSAII DSDNVESGLNNYAENILTLAGSQQTDSDKWQSGLSINNVFKMSSVQKMMQAGKKFKDSLLEPALASVVIH KEATVFDLPKFSVCGSSQESDSLPNSAHDRDRTQDFPESNRLKLLQNAQPPMVTNTARTCPTFSAPVGES ATAKFHVTPLFGNVKKENHSSAKENIGLNVFLSNQSCFPAACENPQRKSFYGSGTIDALSNPILNKACSK TEDNGPKEDSSLPTFKTAKEQLWVDQQKKYHQPQRASGSSYGGVKKSLGASRSRGILGKFVPPIPKQDGG EQNGGMQCKPYGAGPTEPAHPVDERLKNLEPKMIELIMNEIMDHGPPVNWEDIAGVEFAKATIKEIVVWP MLRPDIFTGLRGPPKGILLFGPPGTGKTLIGKCIASQSGATFFSISASSLTSKWVGEGEKMVRALFAVAR CQQPAVIFIDEIDSLLSQRGDGEHESSRRIKTEFLVQLDGATTSSEDRILVVGATNRPQEIDEAARRRLV KRLYIPLPEASARKQIVINLMSKEQCCLSEEEIEQIVQQSDAFSGADMTQLCREASLGPIRSLQTADIAT ITPDQVRPIAYIDFENAFRTVRPSVSPKDLELYENWNKTFGCGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287492
ORF Size	2022 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287492.3
RefSeq Size	3992 bp
RefSeq ORF	2025 bp
Locus ID	63979
UniProt ID	Q6PIW4
MW	74.1 kDa
Gene Summary	This gene encodes a member of the AAA ATPase family of proteins. The encoded protein is recruited to sites of DNA damage where it plays a role in DNA double-strand break repair via homologous recombination. This protein has also been shown to localize to the centrosome and inhibit ciliogenesis, and may regulate the proliferation and differentiation of osteoblasts. [provided by RefSeq, Oct 2016]

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