ZNF133 (NM_001282996) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	pHZ-13; pHZ-66; ZNF150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239278 representing NM_001282996 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACACATGGCATTCAGGGATGTGGCTGTGGATTTCACCCAGGATGAGTGGAGGCTGCTGAGCCCTG CTCAAAGGACTCTGTACAGAGAGGTGATGCTGGAGAACTACAGCAACCTGGTCTCACTGGGAATTTCATT TTCTAAACCAGAACTCATCACCCAGCTGGAGCAAGGGAAAGAGACCTGGAGAGAGGAAAAAAAATGTTCA CCGGCAACCTGTCCAGATCATCAAGAATATGGAAACAACTGTGACCTACAGAAAGAAGCAGATCCAGAGC CAGAGCTCTACCTCGATCCTTTCTGCCCTCCGGGTTTCTCCAGTCAGAAATTCCCCATGCAGCATGTGCT GTGTAATCATCCCCCCTGGATCTTCACATGCTTGTGTGCAGAAGGTAACATCCAGCCTGGGGATCCGGGC CCAGGGGACCAGGAGAAGCAGCAACAAGCCTCTGAGGGGAGACCCTGGAGTGATCAAGCAGAAGGTCCTG AGGGAGAAGGTGCCATGCCTTTGTTTGGAAGAACCAAGAAAAGGACTCTGGGAGCGTTCTCCAGGCCACC CCAGAGGCAGCCAGTCAGCTCTCGGAACGGCCTCAGAGGGGTGGAGTTAGAAGCCAGCCCAGCTCAGACA GGGAACCCTGAGGAAACAGACAAATTGTTGAAGAGGATAGAAGTCTTAGGATTTGGAACAGTCAACTGTG GAGAGTGTGGACTGAGCTTCAGCAAGATGACAAACCTGCTCAGTCACCAGCGGATACACTCAGGGGAGAA GCCCTACGTGTGTGGGGTATGTGAGAAGGGCTTCAGCCTAAAGAAGAGCCTCGCCAGACACCAGAAGGCA CACTCGGGGGAGAAGCCAATTGTGTGCAGGGAGTGTGGACGAGGCTTTAACCGGAAGTCAACGCTAATCA TACACGAACGGACACACTCCGGTGAGAAACCTTACATGTGCAGTGAGTGTGGGCGAGGCTTCAGCCAGAA GTCAAACCTCATCATACACCAGAGGACACACTCAGGGGAAAAGCCTTACGTGTGCCGGGAATGTGGCAAA GGCTTCAGCCAGAAGTCAGCTGTCGTGAGACACCAGAGGACACACTTGGAGGAGAAGACCATCGTGTGCA GTGACTGTGGCCTGGGCTTCAGCGACAGGTCAAACCTCATCTCCCACCAGAGGACGCACTCTGGGGAGAA GCCCTACGCCTGCAAGGAGTGTGGGCGATGCTTCAGGCAGAGGACCACCCTTGTCAACCACCAGAGGACA CACTCAAAGGAGAAGCCCTATGTGTGCGGGGTGTGTGGGCACAGCTTCAGCCAGAATTCAACCCTCATCT CTCACAGGCGGACACACACTGGGGAGAAGCCGTATGTTTGTGGGGTGTGTGGGCGAGGCTTTAGTCTCAA GTCACACCTCAACAGACACCAGAACATACACTCAGGAGAGAAGCCCATTGTGTGCAAGGACTGTGGCCGG GGCTTCAGCCAGCAATCCAACCTCATCAGACACCAGAGGACGCACTCAGGCGAGAAGCCCATGGTGTGTG GGGAGTGCGGGCGAGGCTTCAGCCAGAAGTCAAACCTTGTTGCACACCAGAGGACGCACTCAGGGGAGAG GCCGTATGTGTGCCGAGAGTGCGGGCGAGGCTTTAGCCACCAGGCCGGTCTCATCAGGCACAAGCGGAAG CACTCGAGGGAGAAGCCCTACATGTGCAGGCAGTGTGGACTGGGCTTTGGCAATAAGTCAGCTCTAATTA CACACAAGCGGGCTCACTCGGAAGAGAAGCCTTGTGTGTGCAGAGAGTGTGGCCAAGGCTTTCTCCAAAA GTCACACCTCACCTTACATCAAATGACACATACGGGGGAGAAGCCATATGTGTGCAAGACGTGTGGGCGG GGCTTCAGCCTCAAGTCTCACCTCAGCAGACACAGGAAGACCACGTCTGTCCACCACAGACTGCCAGTGC AGCCCGACCCTGAGCCGTGTGCAGGGCAACCTTCGGATTCCTTATACTCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239278 representing NM_001282996 Red=Cloning site Green=Tags(s) MAHMAFRDVAVDFTQDEWRLLSPAQRTLYREVMLENYSNLVSLGISFSKPELITQLEQGKETWREEKKCS PATCPDHQEYGNNCDLQKEADPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPG PGDQEKQQQASEGRPWSDQAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQT GNPEETDKLLKRIEVLGFGTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKA HSGEKPIVCRECGRGFNRKSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGK GFSQKSAVVRHQRTHLEEKTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRT HSKEKPYVCGVCGHSFSQNSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGR GFSQQSNLIRHQRTHSGEKPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRK HSREKPYMCRQCGLGFGNKSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGR GFSLKSHLSRHRKTTSVHHRLPVQPDPEPCAGQPSDSLYSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282996
ORF Size	2013 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282996.2
RefSeq Size	2610 bp
RefSeq ORF	2016 bp
Locus ID	7692
Protein Families	Transcription Factors
MW	75.9 kDa
Gene Summary	May be involved in transcriptional regulation as a repressor.[UniProtKB/Swiss-Prot Function]

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