VPS52 (NM_001289174) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARE1; dJ1033B10.5; SAC2; SACM2L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239228 representing NM_001289174 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCATCCTGGATGAAGTGGATGGGTGTAGATCTCCGTCACTATTCAAAGCAAGTTGAGCTGGAGC TACAGCAGATTGAACAGAAATCCATTCGGGATTATATTCAAGAGAGTGAGAATATAGCATCTCTACACAA CCAGATCACAGCCTGTGATGCTGTCCTGGAGCGAATGGAGCAGATGTTGGGAGCTTTTCAGAGTGACCTC AGCTCCATCAGCTCTGAGATCCGGACACTGCAGGAACAGTCAGGAGCCATGAACATTCGACTTCGAAATC GCCAGGCAGTTCGGGGGAAACTTGGGGAGCTTGTTGATGGTCTGGTGGTGCCTTCTGCTCTGGTCACGGC AATTCTGGAGGCTCCAGTGACAGAGCCCAGGTTCTTGGAGCAGCTACAGGAGCTGGATGCCAAGGCAGCC GCAGTCAGAGAGCAGGAAGCTAGAGGCACAGCAGCCTGCGCAGATGTCAGAGGCGTGCTCGATCGGCTCC GGGTCAAGGCAGTGACGAAGATCCGAGAGTTTATCCTCCAGAAGATTTATTCCTTCAGGAAACCCATGAC CAACTATCAGATCCCCCAGACGGCCCTGCTGAAGTACAGGTTCTTCTATCAGTTTCTGCTGGGCAATGAA CGAGCAACAGCAAAGGAGATCAGGGATGAATATGTGGAGACGCTGAGCAAGATTTACCTGTCTTACTACC GCTCTTACCTGGGGCGGCTCATGAAGGTGCAGTATGAGGAAGTCGCTGAGAAAGATGATCTAATGGGTGT GGAAGATACAGCAAAGAAAGGATTCTTCTCAAAGCCATCGCTCCGCAGCAGGAACACCATTTTCACCCTA GGAACCCGCGGCTCTGTCATCTCCCCCACTGAACTTGAGGCCCCCATCCTGGTGCCTCACACAGCGCAGC GCGGAGAGCAGAGGTATCCATTTGAGGCCCTCTTCCGCAGCCAGCACTACGCCCTCCTAGACAATTCCTG CCGCGAATACCTTTTCATCTGTGAATTTTTTGTTGTGTCTGGCCCAGCTGCACACGACCTGTTCCATGCT GTCATGGGCCGTACACTCAGCATGACCCTGAAACACCTGGATTCTTATCTAGCTGACTGCTACGATGCCA TTGCTGTTTTTCTCTGTATCCACATTGTTCTCCGGTTCCGTAACATTGCAGCAAAGAGGGATGTTCCTGC CCTGGACAGGTACTGGGAACAGGTGCTTGCCTTGCTATGGCCACGGTTTGAACTGATCCTGGAGATGAAT GTTCAGAGCGTCCGAAGCACTGACCCCCAGCGCCTAGGGGGGTTGGATACTCGGCCCCACTATATCACAC GCCGCTATGCAGAGTTCTCCTCCGCTCTTGTCAGTATCAACCAGACAATTCCTAATGAACGGACCATGCA ATTGCTGGGACAGCTGCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTGGCAGCTGAGTTCTCCTCAAGG AAGGAGCAGCTTGTGTTTCTGATCAACAACTATGACATGATGCTGGGTGTGCTGATGGAGCGGGCTGCAG ATGACAGCAAAGAGGTTGAGAGCTTCCAGCAGCTGCTCAATGCTCGGACACAGGAATTCATTGAAGAGTT GCTGTCTCCCCCTTTTGGGGGTTTAGTGGCATTTGTGAAGGAGGCTGAGGCTTTGATTGAGCGTGGACAG GCTGAGCGACTTCGAGGGGAAGAAGCCCGGGTAACTCAGCTGATCCGTGGCTTTGGTAGTTCCTGGAAAT CATCAGTGGAATCTCTGAGTCAGGATGTAATGCGGAGTTTCACCAACTTCAGAAATGGCACCAGTATCAT TCAGGGAGCGCTGACCCAGCTGATCCAGCTCTATCATCGCTTCCACCGGGTGCTGTCCCAGCCGCAGCTC CGAGCCCTCCCTGCCCGGGCTGAGCTCATCAACATTCACCACCTTATGGTGGAGCTCAAGAAGCATAAGC CCAACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239228 representing NM_001289174 Red=Cloning site Green=Tags(s) MNSSWMKWMGVDLRHYSKQVELELQQIEQKSIRDYIQESENIASLHNQITACDAVLERMEQMLGAFQSDL SSISSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAA AVREQEARGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQTALLKYRFFYQFLLGNE RATAKEIRDEYVETLSKIYLSYYRSYLGRLMKVQYEEVAEKDDLMGVEDTAKKGFFSKPSLRSRNTIFTL GTRGSVISPTELEAPILVPHTAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFVVSGPAAHDLFHA VMGRTLSMTLKHLDSYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMN VQSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTMQLLGQLQVEVENFVLRVAAEFSSR KEQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQ AERLRGEEARVTQLIRGFGSSWKSSVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQL RALPARAELINIHHLMVELKKHKPNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289174
ORF Size	1968 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289174.2
RefSeq Size	3442 bp
RefSeq ORF	1971 bp
Locus ID	6293
MW	75.8 kDa
Gene Summary	This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

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