ZNF189 (NM_001278232) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239081 representing NM_001278232 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTGT ATAAAGATGTCATGATGGAGAATTATGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGATGA GGAGCCAACTGTAAAACAAGAGATTGAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTTACA AGAATCAAAAGTGAAATTGACCAGGATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTAGATA AACAAAGAGGGATCTTCCTATGGGAAATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTCAGAGA AAACACTAACATTATCCGTAAAAGACCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGTGGAAAG GGTTTTGTCCGCAAGGCCCATTTCATTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTTCAGTGCA ATGAATGTGGGAAAAGTTTTAGTCGCAGTTCATTTGTTATTGAACATCAGAGAATTCACACTGGGGAAAG GCCCTATGAGTGTAATTACTGTGGAAAAACCTTTAGTGTGAGCTCAACCCTTATTAGACATCAGAGAATC CACACTGGAGAAAGACCCTATCAGTGTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGGAGCCTTGTTA AACATCAAAGGATTCATACAGGTGAGAAACCCCATAAATGTAGTGACTGTGGGAAAGCCTTCAGTTGGAA ATCACACCTTATTGAGCATCAAAGAACTCACACTGGTGAGAAACCTTATCACTGTACCAAATGTAAGAAG AGCTTTAGTCGAAATTCATTGCTTGTTGAGCATCAAAGAATTCACACTGGGGAAAGACCCCATAAATGTG GTGAATGTGGGAAAGCCTTTCGATTAAGCACATACCTTATACAACACCAAAAAATTCACACTGGCGAGAA GCCTTTTCTTTGTATTGAGTGTGGAAAAAGTTTCAGTCGGAGCTCATTCCTTATTGAACATCAGAGGATC CATACTGGTGAAAGACCTTATCAGTGCAAAGAGTGTGGGAAAAGTTTCAGTCAGCTTTGCAACCTTACTC GTCATCAGAGAATTCACACAGGAGACAAGCCCCATAAATGTGAGGAATGTGGAAAAGCCTTTAGTAGAAG CTCAGGTCTTATTCAGCATCAGAGAATTCACACCAGGGAGAAGACTTATCCATACAATGAAACTAAGGAA AGTTTTGATCCAAATTGCAGTCTTGTTATACAGCAGGAAGTCTACCCTAAGGAGAAATCTTATAAATGTG ATGAATGTGGGAAAACTTTTAGTGTTAGTGCTCATCTTGTACAACATCAAAGAATCCACACTGGTGAAAA GCCCTATCTATGTACTGTCTGTGGGAAAAGCTTCAGCCGGAGCTCATTTCTTATTGAACATCAGAGAATC CACACTGGTGAGAGACCCTATCTGTGCAGACAGTGTGGAAAAAGCTTTAGTCAGCTTTGTAATCTTATTC GACATCAGGGTGTTCACACAGGTAATAAACCCCATAAATGTGATGAATGTGGAAAGGCCTTTAGCCGGAA CTCGGGTCTTATTCAGCATCAGAGAATACACACAGGAGAGAAACCTTATAAGTGTGAGAAGTGCGACAAA AGTTTCAGTCAACAGCGCAGTCTTGTCAACCATCAGAAGATCCATGCAGAGGTGAAAACCCAAGAAACCC ATGAATGTGACGCTTGTGGTGAAGCCTTTAATTGCCGTATTTCTCTTATTCAGCATCAGAAATTGCACAC AGCATGGATGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239081 representing NM_001278232 Red=Cloning site Green=Tags(s) MASPSPPPESKEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEIEEIEEEVEPQGVIVT RIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKRPNSEEKCHKCEECGK GFVRKAHFIQHQRVHTGEKPFQCNECGKSFSRSSFVIEHQRIHTGERPYECNYCGKTFSVSSTLIRHQRI HTGERPYQCNQCKQSFSQRRSLVKHQRIHTGEKPHKCSDCGKAFSWKSHLIEHQRTHTGEKPYHCTKCKK SFSRNSLLVEHQRIHTGERPHKCGECGKAFRLSTYLIQHQKIHTGEKPFLCIECGKSFSRSSFLIEHQRI HTGERPYQCKECGKSFSQLCNLTRHQRIHTGDKPHKCEECGKAFSRSSGLIQHQRIHTREKTYPYNETKE SFDPNCSLVIQQEVYPKEKSYKCDECGKTFSVSAHLVQHQRIHTGEKPYLCTVCGKSFSRSSFLIEHQRI HTGERPYLCRQCGKSFSQLCNLIRHQGVHTGNKPHKCDECGKAFSRNSGLIQHQRIHTGEKPYKCEKCDK SFSQQRSLVNHQKIHAEVKTQETHECDACGEAFNCRISLIQHQKLHTAWMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278232
ORF Size	1833 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278232.2
RefSeq Size	3168 bp
RefSeq ORF	1836 bp
Locus ID	7743
UniProt ID	O75820
Protein Families	Transcription Factors
MW	71.7 kDa
Gene Summary	Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]

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