DOK7 (NM_001301071) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C4orf25; CMS1B; CMS10; FADS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239072 representing NM_001301071 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAGGCGGCGCTGGTGGAGGGCCAGGTCAAGCTGCGGGACGGCAAGAAGTGGAAGAGTAGGTGGC TGGTGCTGCGGAAGCCGTCGCCCGTGGCAGACTGCCTGCTGATGCTGGTCTACAAGGACAAGTCGGAGCG TATCAAGGGCCTGCGGGAGCGCAGCAGCCTGACGCTAGAGGACATCTGCGGGCTGGAGCCCGGCCTGCCC TACGAGGGCCTGGTCCACACGCTGGCCATTGTCTGCCTGTCCCAGGCCATCATGCTGGGCTTTGACAGCC ACGAGGCCATGTGTGCGTGGGATGCCCGGATCCGCTATGCGCTCGGCGAGGTGCATAGGTTCCATGTGAC AGTGGCTCCAGGCACCAAGTTGGAGAGCGGCCCGGCTACCCTGCACCTCTGCAATGATGTCCTCGTCTTG GCCAGGGACATCCCCCCGGCTGTCACGGGGCAGTGGAAGCTGTCTGACCTCCGGCGCTACGGGGCCGTGC CAAGCGGATTCATCTTTGAAGGCGGGACCAGGTGTGGGTACTGGGCTGGCGTCTTCTTCCTGTCCTCGGC CGAGGGGGAGCAGATCAGCTTCCTGTTCGACTGCATCGTCCGAGGCATCTCCCCCACCAAGGGCCCCTTT GGGCTGCGGCCGGTTCTACCAGACCCAAGTCCCCCGGGACCCTCGACTGTGGAGGAGCGTGTGGCCCAGG AAGCCCTGGAAACCCTACAGCTGGAGAAGCGGCTGAGCCTCCTCTCACATGCGGGCAGGCCGGGCAGTGG AGGGGATGACCGCAGCCTGTCCAGCTCATCCTCAGAGGCCAGTCACTTGGACGTCAGCGCCAGCAGCCGG CTCACCGCATGGCCAGAGCAATCCTCGTCGTCAGCCAGCACGTCACAGGAGGGGCCTAGACCAGCAGCTG CCCAGGCCGCCGGGGAAGCCATGGTGGGTGCCTCAAGGCCACCCCCCAAGCCGCTGCGTCCGCGGCAGCT GCAGGAGGTTGGCCGCCAGAGCTCCTCGGACAGCGGCATCGCCACTGGCAGCCACTCCTCTTACTCCAGC AGCCTCTCGTCCTACGCGGGCAGCAGCCTGGACGTGTGGCGGGCCACAGATGAACTGGGCTCACTGCTCA GCCTGCCAGCAGCGGGGGCCCCCGAGCCCAGCCTGTGCACCTGCCTGCCCGGGACAGTCGAGTACCAGGT GCCCACCTCCCTGCGGGCCCACTATGACACACCACGCAGCCTTTGCCTGGCTCCTAGAGACCACAGCCCC CCCTCACAGGGCAGCCCCGGCAACAGTGCGGCCAGGGACTCAGGCGGCCAGACGTCCGCCGGGTGTCCCT CTGGCTGGCTGGGCACGAGACGGCGGGGCCTGGTGATGGAGGCCCCCCAGGGCAGCGAGGCCACACTGCC TGGCCCTGCCCCTGGCGAGCCCTGGGAAGCAGGCGGCCCCCACGCGGGGCCACCCCCGGCTTTCTTTTCG GCATGTCCAGTCTGTGGAGGACTCAAGGGAGCGGCAGCCTCAGCCCCAGGACCTGCGACAGCACATTCAG GATCCCCAGGACCCGTGGCTGTGGACAGCCCAGGACCAGAGAGGCCGCGCGGCGAGTCGCCCACTTACGT GAACATCCCCGTCAGCCCATCCTCCAGAAAGCAGCTGCACTACATGGGCCTGGAGCTCCAGGAGGCCAGC GAGGGTGTCCGAGGGGCTGGCGCCTCCCTCTACGCCCAGATCGACATCATGGCCACCGAGACGGCGCACA GAGTGGGGGTGCGGCACGCACGGGCCCGGGAGGAGCAGCTGTCGGAGCTGGAGCAGAGGAAGGCAGCCCC GCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239072 representing NM_001301071 Red=Cloning site Green=Tags(s) MTEAALVEGQVKLRDGKKWKSRWLVLRKPSPVADCLLMLVYKDKSERIKGLRERSSLTLEDICGLEPGLP YEGLVHTLAIVCLSQAIMLGFDSHEAMCAWDARIRYALGEVHRFHVTVAPGTKLESGPATLHLCNDVLVL ARDIPPAVTGQWKLSDLRRYGAVPSGFIFEGGTRCGYWAGVFFLSSAEGEQISFLFDCIVRGISPTKGPF GLRPVLPDPSPPGPSTVEERVAQEALETLQLEKRLSLLSHAGRPGSGGDDRSLSSSSSEASHLDVSASSR LTAWPEQSSSSASTSQEGPRPAAAQAAGEAMVGASRPPPKPLRPRQLQEVGRQSSSDSGIATGSHSSYSS SLSSYAGSSLDVWRATDELGSLLSLPAAGAPEPSLCTCLPGTVEYQVPTSLRAHYDTPRSLCLAPRDHSP PSQGSPGNSAARDSGGQTSAGCPSGWLGTRRRGLVMEAPQGSEATLPGPAPGEPWEAGGPHAGPPPAFFS ACPVCGGLKGAAASAPGPATAHSGSPGPVAVDSPGPERPRGESPTYVNIPVSPSSRKQLHYMGLELQEAS EGVRGAGASLYAQIDIMATETAHRVGVRHARAREEQLSELEQRKAAPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301071
ORF Size	1824 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301071.2
RefSeq Size	2545 bp
RefSeq ORF	1827 bp
Locus ID	285489
UniProt ID	Q18PE1
MW	64.4 kDa
Gene Summary	The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

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