INSC (NM_001278314) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238925 representing NM_001278314 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCACTGCCTGGAGGTCGCCACCTGGACTCCGTCACCCTGCCGGGTCAGCGGCTACACCTGATGC AGGTGGACTCAGTCCAGCGCTGGATGGAAGATCTGAAGCTCATGACCGAGTGCGAGTGCATGTGTGTCCT GCAGGCCAAGCCCATCAGCCTGGAAGAGGATGCACAGGGTGACCTCATCCTGGCAGGTGGCCCTGGCCCT GGAGACCCCCTGCAGCTGCTGCTCAAACGGGGTTGGGTCATTAGCACAGAGCTGCGCAGGATCGGGCAGA AGCTGGCCCAGGACCGCTGGGCACGGGTGCACAGCATGAGCGTGCGTCTGACCTGCCATGCCCGCTCCAT GGTCAGCGAGTACAGTGCTGTCAGCAGGAACTCCTTGAAGGAAATGGGCGAGATTGAGAAGCTGCTAATG GAGAAATGCTCGGAGCTCTCGGCAGTCACAGAGAGGTGCCTTCAGGTTGAGAATGAGCATGTCCTGAAGT CAATGAAGGCCTGCGTGAGTGAGACCCTGAGCATGCTGGGCCAGCACTTTGGTCAGCTGCTGGAGCTGGC CCTGACACGGGAGGTTCAGGTGGAGTCTCACTCTGTCACCAGGCTGGAGTGCAGTGGTGCAATCTCGGCT CAATGCAACCTCCGCCTCCTGGGTTCAAGTGATTCCCCTGCCTCAGCCTCCCAGGCACTGGTGAGAAAAA TTGATGCCTCAGACAATATCTACACCACAGAGTCCACCACAGGGAACCTGTTCAGCCTGACCCAGGAGGG GGCTCCCTTGTGCCGCATCATAGCCAAGGAGGGTGGGGTCGTAGCACTCTTCAAGGTTTGCCGGCAGGAC AGTTTCCGGTGCTTGTACCCCCAGGCGCTCCGCACGCTGGCCTCCATCTGCTGCGTGGAAGAGGGTGTCC ACCAGCTGGAGAAGGTGGATGGCGTTCTGTGCTTGGCCGACATCCTGACCGACAACAGCCACTCAGAGGC CACACGGGCTGAGGCTGCGGCTGTGGTGGCCCAGGTCACCTCCCCACACCTGCCCGTCACCCAGCACCTC AGTAGCTTCCTGGAGAGCATGGAGGAGATCGTGACAGCCCTCGTCAAACTGTGCCAAGAGGCCTCATCAG GGGAAGTCTTCCTACTGGCCTCTGCGGCCCTTGCCAACATCACGTTCTTTGACACAATGGCCTGCGAGAT GCTCCTGCAGTTGAATGCCATCCGTGTTCTCCTGGAAGCCTGCAGTGACAAGCAGAGAGTGGACACGCCT TACACTCGGGACCAGATTGTGACCATCTTGGCAAACATGTCTGTCCTAGAACAGTGTGCCTCTGACATCA TTCAGGAAAATGGGGTCCAGCTTATCATGGGCATGCTGTCTGAAAAACCAAGGTCTGGGACTCCTGCTGA AGTGGCAGCCTGTGAGCGAGTCCAGCAGAAAGCTGCAGTGACCCTGGCTCGTCTCAGCCGAGACCCAGAT GTGGCACGGGAGGCCGTGCGGCTCAGCTGCATGTCCCGTCTCATCGAGCTCTGCAGATCCCCATCAGAGA GGAACAGCAGTGACGCCGTGCTTGTGGCCTGCCTGGCTGCTCTGCGTAGATTGGCTGGGGTCTGCCCTGA AGGCCTCCAGGACTCTGACTTTCAGCAGTTGGTCCAGCCTCGGCTGGTGGACTCCTTCTTACTCTGCAGC AACATGGAGGAGAGTTTTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238925 representing NM_001278314 Red=Cloning site Green=Tags(s) MMALPGGRHLDSVTLPGQRLHLMQVDSVQRWMEDLKLMTECECMCVLQAKPISLEEDAQGDLILAGGPGP GDPLQLLLKRGWVISTELRRIGQKLAQDRWARVHSMSVRLTCHARSMVSEYSAVSRNSLKEMGEIEKLLM EKCSELSAVTERCLQVENEHVLKSMKACVSETLSMLGQHFGQLLELALTREVQVESHSVTRLECSGAISA QCNLRLLGSSDSPASASQALVRKIDASDNIYTTESTTGNLFSLTQEGAPLCRIIAKEGGVVALFKVCRQD SFRCLYPQALRTLASICCVEEGVHQLEKVDGVLCLADILTDNSHSEATRAEAAAVVAQVTSPHLPVTQHL SSFLESMEEIVTALVKLCQEASSGEVFLLASAALANITFFDTMACEMLLQLNAIRVLLEACSDKQRVDTP YTRDQIVTILANMSVLEQCASDIIQENGVQLIMGMLSEKPRSGTPAEVAACERVQQKAAVTLARLSRDPD VAREAVRLSCMSRLIELCRSPSERNSSDAVLVACLAALRRLAGVCPEGLQDSDFQQLVQPRLVDSFLLCS NMEESFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278314
ORF Size	1701 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278314.1, NP_001265243.1
RefSeq Size	2961 bp
RefSeq ORF	1704 bp
Locus ID	387755
UniProt ID	Q1MX18
MW	62.4 kDa
Gene Summary	In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are tethered by Inscuteable, which regulates their apical localization (Izaki et al., 2006 [PubMed 16458856]).[supplied by OMIM, Mar 2008]

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