PTPN5 (NM_001278236) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PTPSTEP; STEP; STEP61
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238753 representing NM_001278236 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTATGAGGGAGCCAGGAGTGAGAGAGAGAACCACGCTGCTGATGACTCCGAGGGAGGGGCCCTGG ACATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGA AGGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAG AAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCCT CACAGTTCCTGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTGGGGAGTCCCCAGTCTGCTGCTGGT CTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCACCTCCTGAGGACACCCCCAGAGCCA CCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCCAGCCCTCCTTCACCTACTCAGAGT GGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGTGCCCGAGACTCCTGTGTTTGATTG TGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTCAAGTCCATGGGTCTGCAGGAGAGG AGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCTGCAACGAGGAGGGCTTTGGCTATC TCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGCCTCCCGTGTCCTCCAAGCAGAAGA GCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTCTTTGAAATCCCCATGAACTTTGTG GATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGTACAAAACCATACTTCCCAACCCTC ACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAGTTCCTACATCAATGCCAACTACAT CCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGACCCATCGTCAGCACGGTCGCCGAC TTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGATCACCAACATCGAGGAGATGAACG AGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGGTGTTGAGATCACTGTGCAGAAAGT CATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGTGGGACTGAGGAGCGAGGCCTGAAG CATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGGCCCCCCCACTCCTGCACCTGGTGC GGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCCCATCATCGTCCACTGCAGTGCAGG GATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAGCAGCTGCGGCAGGAGGGTGTGGTG GACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCATGATCCAGACATGCGAGCAGTACC AGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCACCAGTCCCCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238753 representing NM_001278236 Red=Cloning site Green=Tags(s) MNYEGARSERENHAADDSEGGALDMCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQ KPPPRGAGSHSLTVRSSLCLFAASQFLLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEP PTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQER RGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFV DPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVAD FWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLK HYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVV DILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278236
ORF Size	1599 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278236.1, NP_001265165.1
RefSeq Size	3939 bp
RefSeq ORF	1602 bp
Locus ID	84867
UniProt ID	P54829
Protein Families	Druggable Genome, Phosphatase, Transmembrane
Protein Pathways	MAPK signaling pathway
MW	60 kDa
Gene Summary	May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function]

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