AER61 (EOGT) (NM_001278689) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AER61; AOS4; C3orf64; EOGT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238728 representing NM_001278689 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAATGTTGTTTGTCTTTGGAGTCTTACTTCATGAAGTCTCACTGAGTGGTCAGAATGAAGCTCCTC CTAATACTCACAGCATTCCAGGCGAACCTCTGTATAACTATGCCAGCATCCGCTTGCCAGAGGAGCACAT TCCCTTCTTTTTGCACAACAATAGGCATATTGCCACTGTCTGTAGGAAAGACTCTCTTTGTCCATATAAG AAACACCTAGAGAAGCTAAAGTACTGCTGGGGTTATGAGAAATCCTGCAAACCAGAGTTCAGGTTTGGTT ACCCAGTTTGCAGCTATGTCGACATGGGATGGACGGACACTCTTGAGTCAGCTGAGGACATATTTTGGAA ACAAGCTGACTTTGGATATGCCAGAGAGAGGCTGGAGGAGATGCATGTGCTCTGTCAGCCTAAGGAAACG AGTGACTCAAGTCTGGTGTGTTCCCGTTATCTTCAGTACTGCAGAGCAACCAATCTCTATCTTGATTTAA GAAACATCAAGAGAAATCATGACAGATTTAAGGAGGACTTTTTCCAGAGTGGTGAAATTGGAGGGCACTG TAAACTTGACATCCGTACATTGACGTCTGAAGGTCAGCGCAAAAGCCCTCTGCAGTCATGGTTTGCTGAG CTACAAAGCTATACTCAGCTCAACTTCAGACCTATAGAAGATGCTAAATGTGACATTGTCATTGAAAAAC CAACATATTTCATGAAATTAGATGCAGGTGTTAACATGTATCACCACTTCTGTGATTTCATCAATCTTTA TATTACTCAGCACGTTAATAACTCATTCAGTACTGACGTGTACATCGTGATGTGGGACACCAGTTCTTAC GGATATGGTGACCTATTCTCCGACACATGGAATGCATTTACTGATTATGACGTTATACATTTGAAAACTT ATGATTCCAAAAGGGTATGTTTTAAAGAAGCTGTTTTTTCATTACTCCCCCGCATGAGGTATGGGCTGTT CTATAATACTCCTCTGATATCTGGCTGTCAAAATACTGGACTATTCAGGGCATTTGCCCAGCATGTACTA CACAGACTAAACATCACACAAGAAGGACCTAAGGATGGAAAAATTCGAGTCACCATTCTTGCACGGAGCA CAGAATACCGGAAAATCCTTAACCAAAATGAGCTTGTAAATGCACTGAAAACAGTATCTACATTTGAAGT CCAGATTGTTGATTACAAGTATAGAGAACTTGGGTTTTTAGATCAACTAAGGATCACACACAACACGGAC ATATTTATTGGAATGCATGGAGCTGGTCTGACCCATTTACTTTTCCTTCCAGACTGGGCTGCTGTATTTG AACTGTACAACTGTGAAGATGAACGCTGTTACTTAGACTTGGCCAGGCTGAGAGGCGTTCACTACATCAC TTGGCGACGGCAGAACAAAGTCTTTCCTCAGGATAAGGGCCACCATCCAACCCTGGGGGAGCACCCGAAG TTCACCAACTACTCTTTCGATGTAGAAGAATTTATGTATCTTGTCCTTCAGGCTGCAGACCACGTATTGC AACACCCAAAGTGGCCATTTAAGAAGAAACATGATGAGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238728 representing NM_001278689 Red=Cloning site Green=Tags(s) MLMLFVFGVLLHEVSLSGQNEAPPNTHSIPGEPLYNYASIRLPEEHIPFFLHNNRHIATVCRKDSLCPYK KHLEKLKYCWGYEKSCKPEFRFGYPVCSYVDMGWTDTLESAEDIFWKQADFGYARERLEEMHVLCQPKET SDSSLVCSRYLQYCRATNLYLDLRNIKRNHDRFKEDFFQSGEIGGHCKLDIRTLTSEGQRKSPLQSWFAE LQSYTQLNFRPIEDAKCDIVIEKPTYFMKLDAGVNMYHHFCDFINLYITQHVNNSFSTDVYIVMWDTSSY GYGDLFSDTWNAFTDYDVIHLKTYDSKRVCFKEAVFSLLPRMRYGLFYNTPLISGCQNTGLFRAFAQHVL HRLNITQEGPKDGKIRVTILARSTEYRKILNQNELVNALKTVSTFEVQIVDYKYRELGFLDQLRITHNTD IFIGMHGAGLTHLLFLPDWAAVFELYNCEDERCYLDLARLRGVHYITWRRQNKVFPQDKGHHPTLGEHPK FTNYSFDVEEFMYLVLQAADHVLQHPKWPFKKKHDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278689
ORF Size	1581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278689.2
RefSeq Size	4666 bp
RefSeq ORF	1584 bp
Locus ID	285203
UniProt ID	Q5NDL2
MW	62.5 kDa
Gene Summary	This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

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