RCBTB2 (NM_001286831) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CHC1L; RLG
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238726 representing NM_001286831 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGATGTGGGAAAGTGGCCAATTTTTTCCCTTTGTTCTGAAGAAGAACTACAGTTAATTCGTCAGG CTTGTGTCTTTGGCAGTGCTGGCAATGAAGTTTTATACACTACAGTAAATGATGAGATTTTTGTGCTTGG CACAAACTGCTGTGGCTGTTTGGGGTTAGGTGACGTCCAGAGCACCATTGAACCTCGGAGACTGGATTCT TTAAATGGCAAAAAAATAGCCTGCCTCAGCTATGGGAGTGGTCCACATATTGTCCTTGCAACAACAGAAG GAGAAGTCTTTACCTGGGGTCATAATGCTTATAGCCAGCTGGGCAATGGGACAACTAATCATGGTTTAGT GCCCTGTCATATCTCTACTAATCTGTCAAACAAACAAGTCATTGAAGTTGCCTGTGGGTCTTACCATTCT TTGGTGCTAACATCTGATGGAGAGGTATTTGCCTGGGGTTATAATAACTCTGGGCAGGTAGGATCTGGAT CAACAGTTAATCAGCCAATCCCTCGAAGAGTCACTGGCTGCCTACAAAATAAAGTAGTTGTGACCATAGC ATGTGGGCAGATGTGCTGCATGGCAGTAGTAGACACGGGGGAGGTCTATGTCTGGGGTTACAACGGAAAC GGGCAGCTTGGACTCGGCAACAGTGGCAACCAGCCAACCCCTTGCAGAGTGGCAGCTTTGCAAGGCATCC GTGTCCAGAGGGTCGCCTGTGGCTACGCACACACATTAGTATTAACAGATGAAGGCCAAGTGTATGCTTG GGGCGCCAATTCTTATGGGCAGTTGGGCACTGGCAATAAAAGCAACCAGTCCTATCCTACTCCTGTCACT GTGGAAAAGGACAGGATTATCGAGATTGCAGCCTGTCACTCCACACACACGTCTGCGGCCAAGACGCAGG GTGGGCACGTGTACATGTGGGGCCAGTGCCGGGGTCAGTCCGTGATCCTCCCGCACCTCACCCACTTCTC CTGCACTGACGACGTGTTTGCCTGCTTTGCCACGCCCGCCGTCACGTGGCGCCTCCTCTCCGTGGAACCT GATGACCACCTCACAGTGGCTGAGTCACTGAAGAGGGAATTTGACAACCCGGACACTGCAGACCTGAAGT TTCTAGTTGATGGAAAGTACATTTATGCACATAAAGTCCTTCTCAAGATTCGATGTGAGCATTTTCGTTC GTCATTGGAAGATAACGAGGATGATATTGTAGAAATGAGTGAATTTTCATATCCTGTTTACCGGGCCTTC CTGGAATACCTATACACAGACAGCATCAGCCTTTCTCCTGAGGAGGCAGTAGGACTGCTAGACTTGGCTA CATTTTATAGAGAAAATCGTTTGAAAAAGCTCTGCCAACAAACTATCAAGCAAGGCATCTGCGAGGAGAA TGCCATCGCTCTGCTCTCGGCTGCGGTGAAGTATGATGCACAGGATTTAGAAGAATTCTGCTTCAGGTTT TGCATAAACCATCTGACTGTAGTAACACAAACATCAGGTTTTGCAGAAATGGACCATGATCTCCTGAAGA ACTTTATCAGCAAAGCAAGCAGAGTTGGAGCCTTTAAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238726 representing NM_001286831 Red=Cloning site Green=Tags(s) MLDVGKWPIFSLCSEEELQLIRQACVFGSAGNEVLYTTVNDEIFVLGTNCCGCLGLGDVQSTIEPRRLDS LNGKKIACLSYGSGPHIVLATTEGEVFTWGHNAYSQLGNGTTNHGLVPCHISTNLSNKQVIEVACGSYHS LVLTSDGEVFAWGYNNSGQVGSGSTVNQPIPRRVTGCLQNKVVVTIACGQMCCMAVVDTGEVYVWGYNGN GQLGLGNSGNQPTPCRVAALQGIRVQRVACGYAHTLVLTDEGQVYAWGANSYGQLGTGNKSNQSYPTPVT VEKDRIIEIAACHSTHTSAAKTQGGHVYMWGQCRGQSVILPHLTHFSCTDDVFACFATPAVTWRLLSVEP DDHLTVAESLKREFDNPDTADLKFLVDGKYIYAHKVLLKIRCEHFRSSLEDNEDDIVEMSEFSYPVYRAF LEYLYTDSISLSPEEAVGLLDLATFYRENRLKKLCQQTIKQGICEENAIALLSAAVKYDAQDLEEFCFRF CINHLTVVTQTSGFAEMDHDLLKNFISKASRVGAFKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286831
ORF Size	1581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286831.1, NP_001273760.1
RefSeq Size	3020 bp
RefSeq ORF	1584 bp
Locus ID	1102
UniProt ID	O95199
MW	58.2 kDa
Gene Summary	This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

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