beta IV Tubulin (TUBB4A) (NM_001289123) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	beta-5; DYT4; TUBB4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238562 representing NM_001289123 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGGGGGGCTGCGGACCGAGAAACTGAGCGGCTCCCGGGGGCGCAGGGACCGTGCTCCGCCGTCT CCGCCGCATCTTCCACCCTCGCCGCCGCCGCAGCTCCCCGCGCTCGTGCCACCGCCGCCGCGTCCACCCT CAGCGCCACCGCCATGCGGGAGATCGTGCACCTGCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCCAAG TTTTGGGAGGTTATCAGTGACGAACATGGCATCGACCCCACAGGCACATACCATGGGGACAGTGACCTGC AACTGGAGAGGATCAACGTGTACTACAACGAGGCCACAGGAGGAAATTATGTCCCCAGAGCGGTGCTGGT GGACCTGGAACCCGGCACCATGGACTCTGTCCGTTCTGGCCCCTTCGGTCAGATCTTTCGGCCGGACAAC TTCGTGTTTGGCCAATCCGGAGCCGGCAACAACTGGGCAAAGGGGCACTACACGGAGGGCGCAGAGCTGG TGGACGCTGTCCTGGACGTAGTCCGGAAGGAGGCCGAGAGCTGCGACTGCCTTCAGGGCTTCCAGCTGAC CCACTCGCTGGGGGGTGGCACGGGGTCCGGAATGGGCACGCTGCTCATCAGTAAGATCCGCGAGGAGTTC CCAGACCGCATCATGAACACCTTCAGCGTGGTGCCCTCGCCCAAAGTGTCAGACACGGTGGTGGAGCCCT ACAACGCCACGCTGTCTGTGCACCAGCTGGTGGAGAATACGGATGAGACCTACTGCATCGACAACGAGGC ACTCTACGACATCTGTTTCCGCACCCTCAAGCTGACCACCCCCACCTACGGGGACCTCAACCACCTGGTG TCGGCCACCATGAGCGGGGTCACCACCTGCCTGCGCTTCCCGGGCCAGCTGAACGCCGACCTGCGCAAGC TGGCCGTCAACATGGTTCCCTTTCCTCGCCTGCACTTCTTCATGCCCGGCTTCGCACCCCTGACCAGCCG GGGCAGCCAGCAGTACCGGGCCCTGACGGTGCCCGAGCTCACCCAGCAGATGTTCGATGCCAAGAACATG ATGGCGGCGTGCGACCCGCGCCACGGCCGCTACCTGACCGTGGCCGCCGTGTTCCGGGGCCGCATGTCCA TGAAGGAGGTGGACGAGCAGATGCTGAGCGTGCAGAGCAAGAACAGCAGCTACTTCGTGGAGTGGATCCC CAACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGATGGCCGCGACCTTCATCGGC AACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACGGCCATGTTCCGGCGCAAGG CCTTCTTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAA TGACCTGGTATCTGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAGGGCGAGTTCGAGGAGGAGGCG GAGGAGGAGGTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238562 representing NM_001289123 Red=Cloning site Green=Tags(s) MRRGAADRETERLPGAQGPCSAVSAASSTLAAAAAPRARATAAASTLSATAMREIVHLQAGQCGNQIGAK FWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDN FVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEF PDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLV SATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNM MAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIG NSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEA EEEVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289123
ORF Size	1485 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289123.1, NP_001276052.1
RefSeq Size	2501 bp
RefSeq ORF	1488 bp
Locus ID	10382
UniProt ID	P04350
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection
MW	54.9 kDa
Gene Summary	This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.