DDX52 (NM_001291476) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HUSSY19; ROK1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238532 representing NM_001291476 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTGTAGAAGCAAAGATTGAAGACAAAAAAGTTCAGAGAGAAAGTAAACTAACTTCCGGAAAGT TGGAGAATCTCAGAAAAGAAAAGATAAACTTCTTGCGGAATAAACACAAAATTCACGTCCAAGGAACCGA TCTTCCTGACCCAATTGCTACATTTCAGCAACTTGACCAGGAATATAAAATCAATTCTCGACTACTTCAG AACATTCTAGATGCAGGTTTCCAAATGCCTACGCCAATCCAAATGCAAGCCATCCCAGTTATGCTGCATG GTCGGGAACTTCTGGCTTCTGCTCCAACTGGATCTGGAAAAACATTAGCTTTTAGCATTCCTATTTTAAT GCAGCTGAAACAACCCGCAAATAAAGGCTTCAGAGCCCTGATTATATCACCAACACGAGAACTTGCCAGC CAGATTCACAGAGAGTTAATAAAAATTTCTGAGGGAACAGGATTCAGAATACACATGATCCACAAAGCAG CAGTGGCAGCCAAGAAATTTGGACCTAAATCATCTAAAAAGTTTGATATTCTTGTGACTACTCCAAATCG ACTAATCTATTTATTAAAGCAAGATCCCCCCGGAATCGACCTAGCAAGTGTTGAGTGGCTTGTAGTAGAC GAATCAGATAAACTGTTTGAAGATGGCAAAACTGGGTTCAGAGACCAGCTGGCTTCCATTTTCCTGGCCT GCACATCCCACAAGGTCCGAAGAGCTATGTTCAGTGCAACTTTTGCATATGATGTTGAACAGTGGTGCAA ACTCAACCTGGACAATGTCATCAGTGTGTCCATTGGAGCAAGGAATTCTGCAGTAGAAACTGTAGAACAA GAGCTTCTCTTTGTTGGATCTGAGACCGGAAAACTTCTGGCCGTGAGAGAACTTGTTAAAAAGGGTTTCA ATCCACCTGTTCTTGTTTTTGTTCAGTCCATTGAAAGGGCTAAAGAACTTTTTCATGAGCTCATATATGA AGGTATTAATGTGGATGTTATTCATGCAGAGAGAACACAACAACAGAGAGATAACACAGTCCACAGTTTC AGAGCAGGAAAAATCTGGGTTCTGATTTGTACAGCCTTGCTAGCAAGAGGGATTGATTTTAAAGGTGTGA ACTTGGTGATCAACTATGACTTTCCAACTAGCTCAGTGGAATATATCCACAGGATAGGTCGAACTGGAAG AGCAGGGAATAAGGGAAAAGCAATTACATTTTTCACTGAGGATGATAAGCCATTATTAAGAAGCGTTGCT AATGTTATACAGCAGGCTGGGTGTCCTGTACCAGAATACATAAAAGGTTTTCAGAAACTACTAAGCAAAC AAAAGAAAAAGATGATTAAGAAACCATTGGAAAGGGAGAGCATTAGTACAACTCCAAAATGTTTCTTAGA AAAAGCTAAGGATAAACAGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAA AGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238532 representing NM_001291476 Red=Cloning site Green=Tags(s) MSSVEAKIEDKKVQRESKLTSGKLENLRKEKINFLRNKHKIHVQGTDLPDPIATFQQLDQEYKINSRLLQ NILDAGFQMPTPIQMQAIPVMLHGRELLASAPTGSGKTLAFSIPILMQLKQPANKGFRALIISPTRELAS QIHRELIKISEGTGFRIHMIHKAAVAAKKFGPKSSKKFDILVTTPNRLIYLLKQDPPGIDLASVEWLVVD ESDKLFEDGKTGFRDQLASIFLACTSHKVRRAMFSATFAYDVEQWCKLNLDNVISVSIGARNSAVETVEQ ELLFVGSETGKLLAVRELVKKGFNPPVLVFVQSIERAKELFHELIYEGINVDVIHAERTQQQRDNTVHSF RAGKIWVLICTALLARGIDFKGVNLVINYDFPTSSVEYIHRIGRTGRAGNKGKAITFFTEDDKPLLRSVA NVIQQAGCPVPEYIKGFQKLLSKQKKKMIKKPLERESISTTPKCFLEKAKDKQKKVTGQNSKKKVALEDK S TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291476
ORF Size	1473 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291476.2
RefSeq Size	6556 bp
RefSeq ORF	1476 bp
Locus ID	11056
UniProt ID	Q9Y2R4
MW	55.6 kDa

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