C19orf28 (MFSD12) (NM_001287529) Human Tagged ORF Clone
CAT#: RC238422
- TrueORF®
MFSD12 (myc-DDK-tagged) - Human major facilitator superfamily domain containing 12 (MFSD12), transcript variant 4
ORF Plasmid: tGFP
"NM_001287529" in other vectors (2)
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CNY 4,660.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C19orf28; PP3501 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238422 representing NM_001287529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTGGCGCGGCTGA GCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTCACCTACCTGCTGCTCTACCT GCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTGCTGCTGCTGCTGGGCCAGGTGGCCGACGGG CTGTGCACACCGCTCGTGGGCTACGAGGCCGACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCACCGTCT GCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCGGCCACGCCCGAGTGGGC TGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTTGGCTGGGCCTCCACACAGATCTCCCAC CTCAGCCTCATCCCGGAGCTCGTCACCAACGACCATGAGAAGGTGGAGCTCACGGCACTCAGGTATGCGT TCACCGTGGTGGCCAACATCACCGTCTACGGCGCCGCCTGGCTCCTGCTGCACCTGCAGGGCTCGTCGCG GGTGGAGCCCACCCAAGACATCAGCATCAGCGACCAGCTGGGGGGCCAGGACGTGCCCGTGTTCCGGAAC CTGTCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGCTATTCCACCTGGGCACCCGGGAGAGGC GCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTTGGCCCCTGCCACGGCCCAGCCCCTGCT GCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTGGGCATACTGTACATGACCACCAGGCTC ATCGTGAACCTGTCCCAGACCTACATGGCCATGTACCTCACCTACTCGCTCCACCTGCCCAAGAAGTTCA TCGCGACCATTCCCCTGGTGATGTACCTCAGCGGCTTCTTGTCCTCCTTCCTCATGAAGCCCATCAACAA GTGCATTGGGAGGAACATGACCTACTTCTCAGGCCTCCTGGTGATCCTGGCCTTTGCCGCCTGGGTGGCG CTGGCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGCTGCTGGGTGCTGGCTGTGCCACCATCC TCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCACACGAACAGCGGAGCGTTCGTGTACGG CTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTCATGGCCATCCAGAGCCTGCACCCTTGC CCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGCTTTTACCACTGGGCGATGGTGGCTGTGACGGGCGGCG TGGGCGTGGCCGCTGCCCTGTGTCTCTGTAGCCTCCTGCTGTGGCCGACCCGCCTGCGACGCTGGGACCG TGATGCCCGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238422 representing NM_001287529
Red=Cloning site Green=Tags(s) MGPGPPAAGAAPSPRPLSLVARLSYAVGHFLNDLCASMWFTYLLLYLHSVRAYSSRGAGLLLLLGQVADG LCTPLVGYEADRAASCCARYGTVCVLLSFPFIFSPCLGCGAATPEWAALLYYGPFIVIFQFGWASTQISH LSLIPELVTNDHEKVELTALRYAFTVVANITVYGAAWLLLHLQGSSRVEPTQDISISDQLGGQDVPVFRN LSLLVVGVGAVFSLLFHLGTRERRRPHAEEPGEHTPLLAPATAQPLLLWKHWLREPAFYQVGILYMTTRL IVNLSQTYMAMYLTYSLHLPKKFIATIPLVMYLSGFLSSFLMKPINKCIGRNMTYFSGLLVILAFAAWVA LAEGLGVAVYAAAVLLGAGCATILVTSLAMTADLIGPHTNSGAFVYGSMSFLDKVANGLAVMAIQSLHPC PSELCCRACVSFYHWAMVAVTGGVGVAAALCLCSLLLWPTRLRRWDRDARP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001287529 |
ORF Size | 1413 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001287529.1, NP_001274458.1 |
RefSeq Size | 2122 bp |
RefSeq ORF | 1416 bp |
Locus ID | 126321 |
UniProt ID | Q6NUT3 |
Protein Families | Transmembrane |
MW | 51.5 kDa |
Gene Summary | Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin pigmentation (PubMed:33208952). In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin pigmentation (PubMed:33208952). Also catalyzes import of cysteine into lysosomes in non-pigmented cells (PubMed:33208952).[UniProtKB/Swiss-Prot Function] |
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