WSB2 (NM_001278557) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SBA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238123 representing NM_001278557 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGTAGACAGGGAGAGCAGGTTTCTCAGGGGGACGGGGACAGGCGAGGCTGTCGCCGTAGAGGAAC CGCTGCTGCTGGCCGAACTCAAGCCCGGGCGCCCCCACCAGTTTGATTGGAAGTCCAGCTGTGAAACCTG GAGCGTCGCCTTCTCCCCAGATGGCTCCTGGTTTGCTTGGTCTCAAGGACACTGCATCGTCAAACTGATC CCCTGGCCGTTGGAGGAGCAGTTCATCCCTAAAGGGTTTGAAGCCAAAAGCCGAAGTAGCAAAAATGAGA CGAAAGGGCGGGGCAGCCCAAAAGAGAAGACGCTGGACTGTGGTCAGATTGTCTGGGGGCTGGCCTTCAG CCCGTGGCCTTCCCCACCCAGCAGGAAGCTCTGGGCACGCCACCACCCCCAAGTGCCCGATGTCTCTTGC CTGGTTCTTGCTACGGGACTCAACGATGGGCAGATCAAGATCTGGGAGGTGCAGACAGGGCTCCTGCTTT TGAATCTTTCCGGCCACCAAGATGTCGTGAGAGATCTGAGCTTCACACCCAGTGGCAGTTTGATTTTGGT CTCCGCGTCACGGGATAAGACTCTTCGCATCTGGGACCTGAATAAACACGGTAAACAGATTCAAGTGTTA TCGGGCCACCTGCAGTGGGTTTACTGCTGTTCCATCTCCCCAGACTGCAGCATGCTGTGCTCTGCAGCTG GAGAGAAGTCGGTCTTTCTATGGAGCATGAGGTCCTACACGTTAATTCGGAAGCTAGAGGGCCATCAAAG CAGTGTTGTCTCTTGTGACTTCTCCCCCGACTCTGCCCTGCTTGTCACGGCTTCTTACGATACCAATGTG ATTATGTGGGACCCCTACACCGGCGAAAGGCTGAGGTCACTCCACCACACCCAGGTTGACCCCGCCATGG ATGACAGTGACGTCCACATTAGCTCACTGAGATCTGTGTGCTTCTCTCCAGAAGGCTTGTACCTTGCCAC GGTGGCAGATGACAGACTCCTCAGGATCTGGGCCCTGGAACTGAAAACTCCCATTGCATTTGCTCCTATG ACCAATGGGCTTTGCTGCACATTTTTTCCACATGGTGGAGTCATTGCCACAGGGACAAGAGATGGCCACG TCCAGTTCTGGACAGCTCCTAGGGTCCTGTCCTCACTGAAGCACTTATGCCGGAAAGCCCTTCGAAGTTT CCTAACAACTTACCAAGTCCTAGCACTGCCAATCCCCAAGAAAATGAAAGAGTTCCTCACATACAGGACT TTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238123 representing NM_001278557 Red=Cloning site Green=Tags(s) MRVDRESRFLRGTGTGEAVAVEEPLLLAELKPGRPHQFDWKSSCETWSVAFSPDGSWFAWSQGHCIVKLI PWPLEEQFIPKGFEAKSRSSKNETKGRGSPKEKTLDCGQIVWGLAFSPWPSPPSRKLWARHHPQVPDVSC LVLATGLNDGQIKIWEVQTGLLLLNLSGHQDVVRDLSFTPSGSLILVSASRDKTLRIWDLNKHGKQIQVL SGHLQWVYCCSISPDCSMLCSAAGEKSVFLWSMRSYTLIRKLEGHQSSVVSCDFSPDSALLVTASYDTNV IMWDPYTGERLRSLHHTQVDPAMDDSDVHISSLRSVCFSPEGLYLATVADDRLLRIWALELKTPIAFAPM TNGLCCTFFPHGGVIATGTRDGHVQFWTAPRVLSSLKHLCRKALRSFLTTYQVLALPIPKKMKEFLTYRT F TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278557
ORF Size	1263 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278557.1, NP_001265486.1
RefSeq Size	2798 bp
RefSeq ORF	1266 bp
Locus ID	55884
UniProt ID	Q9NYS7
Protein Families	Druggable Genome
MW	47.6 kDa
Gene Summary	This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

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