WNT8A (NM_001300939) Human Tagged ORF Clone
CAT#: RC237778
- TrueORF®
WNT8A (myc-DDK-tagged) - Human wingless-type MMTV integration site family, member 8A (WNT8A), transcript variant 2
ORF Plasmid: tGFP
"NM_001300939" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | WNT8D |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237778 representing NM_001300939
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGCTGCATTCAGTGCCTCTGCCTGGTAAGTCCTTTCCCAACCCTCACTCCTTGCCAAGGAGGCC CCCATTGTCTCATCCCCATTCACCTCTGCCTCACTTTTTCTCTTTTTGGTAGGTCAGTGAACAATTTCCT GATAACAGGTCCCAAGGCCTATCTGACCTACACGACTAGTGTGGCCTTGGGTGCCCAGAGTGGCATCGAG GAGTGCAAGTTCCAGTTTGCTTGGGAACGCTGGAACTGCCCTGAAAATGCTCTTCAGCTCTCCACCCACA ACAGGCTGAGAAGTGCTACCAGAGAGACTTCCTTCATACATGCTATCAGCTCTGCTGGAGTCATGTACAT CATCACCAAGAACTGTAGCATGGGTGACTTCGAAAACTGTGGCTGTGATGGGTCAAACAATGGAAAAACA GGAGGCCATGGCTGGATCTGGGGAGGCTGCAGCGACAATGTGGAATTTGGGGAAAGGATCTCCAAACTCT TTGTGGACAGTTTGGAGAAGGGGAAGGATGCCAGAGCCCTGATGAATCTTCACAACAACAGGGCCGGCAG ACTGGCAGTGAGAGCCACCATGAAAAGGACATGCAAATGTCATGGCATCTCTGGGAGCTGCAGCATACAG ACATGCTGGCTGCAGCTGGCTGAATTCCGGGAGATGGGAGACTACCTAAAGGCCAAGTATGACCAGGCGC TGAAAATTGAAATGGATAAGCGGCAGCTGAGAGCTGGGAACAGCGCCGAGGGCCACTGGGTGCCCGCTGA GGCCTTCCTTCCTAGCGCAGAGGCGGAACTGATCTTTTTAGAGGAATCACCAGATTACTGTACCTGCAAT TCCAGCCTGGGCATCTATGGCACAGAGGGTCGTGAGTGCCTACAGAACAGCCACAACACATCCAGGTGGG AGCGACGTAGCTGTGGGCGCCTGTGCACTGAGTGTGGGCTGCAGGTGGAAGAGAGGAAAACTGAGGTCAT AAGCAGCTGTAACTGCAAATTCCAGTGGTGCTGTACGGTCAAGTGTGACCAGTGTAGGCATGTGGTGAGC AAGTATTACTGCGCACGCTCCCCAGGCAGTGCCCAGTCCCTGGGTAAGGGCAGTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237778 representing NM_001300939
Red=Cloning site Green=Tags(s) MLCCIQCLCLVSPFPTLTPCQGGPHCLIPIHLCLTFSLFGRSVNNFLITGPKAYLTYTTSVALGAQSGIE ECKFQFAWERWNCPENALQLSTHNRLRSATRETSFIHAISSAGVMYIITKNCSMGDFENCGCDGSNNGKT GGHGWIWGGCSDNVEFGERISKLFVDSLEKGKDARALMNLHNNRAGRLAVRATMKRTCKCHGISGSCSIQ TCWLQLAEFREMGDYLKAKYDQALKIEMDKRQLRAGNSAEGHWVPAEAFLPSAEAELIFLEESPDYCTCN SSLGIYGTEGRECLQNSHNTSRWERRSCGRLCTECGLQVEERKTEVISSCNCKFQWCCTVKCDQCRHVVS KYYCARSPGSAQSLGKGSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001300939 |
ORF Size | 1107 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300939.2 |
RefSeq Size | 1814 bp |
RefSeq ORF | 1110 bp |
Locus ID | 7478 |
UniProt ID | Q9H1J5 |
Protein Families | Cancer stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
MW | 41.3 kDa |
Gene Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014] |
Documents
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SDS |