SEC13L1 (SEC13) (NM_001136026) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D3S1231E; npp-20; SEC13L1; SEC13R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237769 representing NM_001136026 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAACCTGTGCTTACTTGGTGTGTACCTCTTGAACTGCTTTGCAGCCATCCTCTTCCACTGTCTG CCTTTCTGAAATCACAAGTCAAACTTTATACCTACAGGGCTTGTGCTGGAAAAGATGAAATGGGGAAAAT GGTGTCAGTAATTAACACTGTGGATACCTCCCATGAGGACATGATTCACGACGCCCAGATGGACTACTAT GGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTCAAAATCTTTGATGTGCGCAATGGAGGGCAGA TCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTGTGGCAAGTGGCCTGGGCTCACCCCATGTACGG CAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTCATTATCTGGAGAGAGGAAAACGGCACCTGGGAG AAGAGCCACGAGCATGCGGGACACGACTCCTCAGTGAACTCGGTGTGCTGGGCCCCCCATGACTACGGCC TGATCCTGGCCTGTGGGAGCTCGGATGGGGCCATCTCCCTGCTGACTTACACCGGGGAAGGCCAATGGGA AGTAAAGAAGATCAACAACGCTCACACCATTGGCTGCAATGCCGTCAGCTGGGCCCCTGCTGTTGTACCT GGAAGCCTCATAGACCACCCATCGGGGCAGAAACCCAATTACATCAAGAGGTTTGCATCAGGTGGCTGTG ACAACCTCATCAAGCTGTGGAAGGAGGAGGAGGACGGCCAGTGGAAGGAGGAGCAGAAGCTAGAAGCGCA CAGTGACTGGGTTCGAGATGTGGCCTGGGCCCCCTCCATCGGCCTGCCCACCAGCACCATCGCCAGCTGC TCCCAGGATGGTCGTGTGTTCATTTGGACCTGTGATGATGCCTCAAGCAATACGTGGTCCCCTAAATTGT TGCACAAGTTCAACGATGTGGTGTGGCATGTGAGCTGGTCCATCACAGCCAACATCCTGGCTGTCTCTGG TGGAGACAATAAGGTGACCCTGTGGAAGGAGTCAGTTGATGGGCAGTGGGTGTGCATCAGTGATGTCAAC AAGGGCCAGGGCTCCGTATCAGCATCAGTGACAGAGGGCCAGCAGAACGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237769 representing NM_001136026 Red=Cloning site Green=Tags(s) MREPVLTWCVPLELLCSHPLPLSAFLKSQVKLYTYRACAGKDEMGKMVSVINTVDTSHEDMIHDAQMDYY GTRLATCSSDRSVKIFDVRNGGQILIADLRGHEGPVWQVAWAHPMYGNILASCSYDRKVIIWREENGTWE KSHEHAGHDSSVNSVCWAPHDYGLILACGSSDGAISLLTYTGEGQWEVKKINNAHTIGCNAVSWAPAVVP GSLIDHPSGQKPNYIKRFASGGCDNLIKLWKEEEDGQWKEEQKLEAHSDWVRDVAWAPSIGLPTSTIASC SQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVDGQWVCISDVN KGQGSVSASVTEGQQNEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001136026
ORF Size	1104 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001136026.3
RefSeq Size	1615 bp
RefSeq ORF	1107 bp
Locus ID	6396
UniProt ID	P55735
MW	41.2 kDa
Gene Summary	The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]

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