ZNF140 (NM_001300778) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	pHZ-39
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237677 representing NM_001300778 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGAATTCTAAGTCAAGGCCCTGTGTATTCCAGTTTTAAAGGAGGCTGGAAATGCAAGGATCATA CTGAGATGCTGCAAGAAAATCAGGGATGTATTAGGAAAGTAACAGTCTCTCATCAAGAAGCCCTGGCTCA ACATATGAATATCAGTACTGTGGAGAGGCCCTATGGATGCCATGAATGTGGAAAAACTTTTGGTCGACGC TTTTCCCTGGTGTTACACCAGAGGACTCATACTGGAGAGAAACCATATGCATGTAAGGAATGTGGCAAAA CCTTTAGCCAGATTTCAAACCTTGTGAAACACCAAATGATACATACTGGAAAGAAACCCCATGAGTGTAA GGACTGTAATAAAACATTCAGTTACCTTTCATTTCTTATTGAACACCAGAGAACGCACACTGGGGAGAAA CCTTATGAATGTACTGAGTGTGGAAAGGCCTTTAGCCGTGCCTCCAACCTCACTCGACATCAAAGAATTC ACATAGGAAAGAAACAATATATATGTAGGAAATGTGGTAAAGCATTTAGCAGTGGCTCAGAACTCATTCG CCACCAGATTACACATACTGGAGAGAAACCTTATGAATGCATTGAATGTGGGAAGGCATTTCGCCGTTTC TCACACCTTACTCGACATCAGAGCATCCATACAACCAAAACCCCGTATGAATGTAATGAATGTAGGAAAG CTTTCCGTTGTCACTCATTCCTTATTAAACATCAGAGAATTCATGCTGGAGAAAAGCTCTATGAATGTGA TGAATGTGGTAAAGTTTTCACTTGGCATGCATCCCTTATTCAACATACGAAGAGTCACACTGGAGAGAAA CCCTATGCGTGTGCTGAATGTGATAAAGCCTTCAGCCGGAGCTTTTCCCTCATTCTACATCAGAGAACTC ATACTGGAGAGAAACCCTATGTATGTAAGGTATGCAACAAATCCTTCAGCTGGAGCTCAAACCTTGCTAA ACATCAGAGGACACACACTCTTGACAACCCCTATGAATATGAAAATTCATTTAATTACCACTCATTCCTT ACTGAACACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237677 representing NM_001300778 Red=Cloning site Green=Tags(s) MERILSQGPVYSSFKGGWKCKDHTEMLQENQGCIRKVTVSHQEALAQHMNISTVERPYGCHECGKTFGRR FSLVLHQRTHTGEKPYACKECGKTFSQISNLVKHQMIHTGKKPHECKDCNKTFSYLSFLIEHQRTHTGEK PYECTECGKAFSRASNLTRHQRIHIGKKQYICRKCGKAFSSGSELIRHQITHTGEKPYECIECGKAFRRF SHLTRHQSIHTTKTPYECNECRKAFRCHSFLIKHQRIHAGEKLYECDECGKVFTWHASLIQHTKSHTGEK PYACAECDKAFSRSFSLILHQRTHTGEKPYVCKVCNKSFSWSSNLAKHQRTHTLDNPYEYENSFNYHSFL TEHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300778
ORF Size	1062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300778.1, NP_001287707.1
RefSeq Size	3394 bp
RefSeq ORF	1065 bp
Locus ID	7699
UniProt ID	P52738
Protein Families	Transcription Factors
MW	41.6 kDa
Gene Summary	May be involved in transcriptional regulation as a repressor.[UniProtKB/Swiss-Prot Function]

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