ZKSCAN1 (NM_001287055) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KOX18; PHZ-37; ZNF36; ZNF139; ZSCAN33
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237638 representing NM_001287055 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTGCACTATTCACAGCGGATTCCCAGGCAATGGTGAAGATCGAGGACATGGCTGTGTCCCTCA TTCTGGAGGAATGGGGATGTCAGAATCTGGCTCGGAGGAATCTCAGTAGGGACAACAGGCAGGAGAATTA TGGGAGCGCATTTCCCCAGGGTGGTGAAAACAGGAATGAGAACGAGGAGTCAACCTCAAAGGCTGAAACC TCGGAAGATTCAGCATCACGCGGGGAGACAACAGGAAGATCCCAGAAAGAGTTTGGAGAGAAACGTGACC AGGAGGGCAAAACAGGAGAAAGACAGCAGAAAAACCCTGAGGAGAAAACCAGGAAAGAGAAAAGAGATTC AGGGCCAGCTATAGGAAAGGACAAAAAAACCATCACAGGAGAGAGAGGTCCAAGGGAGAAGGGGAAAGGA TTGGGAAGAAGCTTCAGTCTGAGCTCCAACTTCACCACCCCTGAAGAAGTTCCCACGGGAACAAAGTCTC ACAGATGTGATGAATGTGGTAAATGCTTCACGAGAAGTTCAAGCCTTATCCGCCATAAAATAATCCACAC TGGAGAAAAGCCCTATGAATGTAGTGAGTGTGGGAAAGCCTTCAGTCTTAACTCCAACCTTGTCCTGCAT CAGAGGATCCACACAGGAGAGAAACCTCATGAATGTAACGAGTGTGGCAAGGCCTTCAGCCACAGTTCCA ATCTCATCCTCCATCAGCGCATCCACTCTGGAGAGAAACCTTATGAATGTAATGAGTGCGGGAAGGCCTT CAGCCAGAGCTCGGACCTCACCAAGCATCAGAGAATTCACACGGGGGAGAAACCCTATGAATGTAGTGAA TGTGGAAAAGCTTTCAACCGAAACTCATACCTGATTTTGCATCGGAGAATTCACACTCGAGAAAAGCCCT ACAAGTGCACTAAGTGTGGCAAGGCCTTCACCCGCAGCTCCACCCTCACTCTGCATCACAGAATCCATGC CAGAGAGAGAGCCTCTGAGTACAGCCCAGCCTCCCTTGATGCATTTGGCGCGTTCCTGAAAAGTTGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237638 representing NM_001287055 Red=Cloning site Green=Tags(s) MASALFTADSQAMVKIEDMAVSLILEEWGCQNLARRNLSRDNRQENYGSAFPQGGENRNENEESTSKAET SEDSASRGETTGRSQKEFGEKRDQEGKTGERQQKNPEEKTRKEKRDSGPAIGKDKKTITGERGPREKGKG LGRSFSLSSNFTTPEEVPTGTKSHRCDECGKCFTRSSSLIRHKIIHTGEKPYECSECGKAFSLNSNLVLH QRIHTGEKPHECNECGKAFSHSSNLILHQRIHSGEKPYECNECGKAFSQSSDLTKHQRIHTGEKPYECSE CGKAFNRNSYLILHRRIHTREKPYKCTKCGKAFTRSSTLTLHHRIHARERASEYSPASLDAFGAFLKSCV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287055
ORF Size	1050 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287055.2
RefSeq Size	8776 bp
RefSeq ORF	1053 bp
Locus ID	7586
Protein Families	Transcription Factors
MW	39.8 kDa
Gene Summary	This gene encodes a member of the Kruppel C2H2-type zinc-finger family of proteins. This encoded protein may function as a transcription factor that regulates the expression of GABA type-A receptors in the brain. Transcripts from this gene have been shown to form stable and abundant circular RNAs. Elevated expression of this gene has been observed in gastric cancer and the encoded protein may stimulate migration and invasion of human gastric cancer cells. [provided by RefSeq, Oct 2016]

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