NRF1 (NM_001293164) Human Tagged ORF Clone
CAT#: RC237588
- TrueORF®
NRF1 (myc-DDK-tagged) - Human nuclear respiratory factor 1 (NRF1), transcript variant 4
ORF Plasmid: tGFP
"NM_001293164" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALPHA-PAL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237588 representing NM_001293164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCTGGAAGACCTGGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAACTCAGAAC TGCCGCCTCTCACCATCGACGGAATTCCAGTCTCTGTGGACAAAATGACCCAGGCCCAGCTTCGGGCATT TATCCCAGAGATGCTCAAGTACTCTACAGGTCGGGGAAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCC ATCTGGTGGCCTGAAGATATCCCCTGGGCAAATGTCCGGAGTGATGTCCGCACAGAAGAGCAAAAGCAGA GGGTTTCATGGACCCAGGCACTACGGACCATAGTTAAAAACTGTTATAAACAGCATGGGCGGGAAGACCT TTTGTATGCCTTTGAAGATCAGCAAACGCAAACACAGGCCACAGCCACACATAGTATAGCTCATCTTGTA CCATCACAGACTGTAGTCCAGACTTTTAGTAACCCTGATGGCACTGTCTCACTTATCCAGGTTGGTACGG GGGCAACAGTAGCCACATTGGCTGATGCTTCAGAATTGCCAACCACGGTCACCGTTGCCCAAGTGAATTA TTCTGCCGTGGCTGATGGAGAGGTGGAACAAAATTGGGCCACGTTACAGGGAGGTGAGATGACCATCCAG ACGACGCAAGCATCAGAGGCCACCCAGGCGGTGGCATCGTTGGCAGAGGCCGCAGTGGCAGCTTCTCAGG AGATGCAGCAGGGAGCTACAGTCACTATGGCGCTTAACAGCGAAGCTGCCGCCCATGCTGTCGCCACCCT GGCTGAGGCCACCTTACAAGGTGGGGGACAGATCGTCTTGTCTGGGGAAACCGCAGCAGCCGTCGGAGCA CTTACTGGAGTCCAAGATGCTAATGGCCTGGTCCAGATCCCTGTGAGCATGTACCAGACTGTGGTGACCA GCCTCGCCCAGGGCAACGGACCAGTGCAGGTGGCCATGGCCCCTGTGACCACCAGGATATCAGACAGCGC AGTCACCATGGACGGCCAAGCTGTGGAGGTGGTGACATTGGAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237588 representing NM_001293164
Red=Cloning site Green=Tags(s) MILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKP IWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLV PSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQ TTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGA LTGVQDANGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001293164 |
ORF Size | 1026 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001293164.2 |
RefSeq Size | 3440 bp |
RefSeq ORF | 1029 bp |
Locus ID | 4899 |
UniProt ID | Q16656 |
Protein Families | Transcription Factors |
Protein Pathways | Huntington's disease |
MW | 36.5 kDa |
Gene Summary | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] |
Documents
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FAQs |
SDS |