RPP40 (NM_001286133) Human Tagged ORF Clone
CAT#: RC237427
- TrueORF®
RPP40 (myc-DDK-tagged) - Human ribonuclease P/MRP 40kDa subunit (RPP40), transcript variant 3
ORF Plasmid: tGFP
"NM_001286133" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | bA428J1.3; RNASEP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237427 representing NM_001286133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACGCTGCGCCGGCTTCGGGAGGCGCCGCGGCACTTACTGGTTTGCGAGAAATCCAACTTCGGCA ACCACAAGTCGCGCCACCGGCATCTTGTGCAGACGCACTACTATAACTACAGGGTTTCATTTCTCATTCC TGAATGTGGGATACTATCGGAAGAACTGAAAAACCTGGTCATGAACACTGGACCCTATTACTTTGTGAAG AATTTACCTCTTCATGAATTAATTACACCTGAATTCATCAGTACCTTTATAAAGAAAGGTTCTTGCTATG CACTAACATACAATACACATATTGATGAAGATAATACTGTTGCCCTGCTACCAAATGGGAAATTAATTTT GTCACTGGATAAAGACACTTATGAAGAAACTGGACTTCAGGGTCATCCATCTCAGTTTTCTGGCAGAAAA ATTATGAAATTTAGTTCAGAAGAATCGACAATGATGTCATATTTTTCCAAGTACCAAATTCAGGAGCATC AGCCAAAAGTAGCACTGAGCACGTTGAGAGATCTCCAGTGCCCAGTGCTGCAGAGCAGCGAGCTGGAGGG AACGCCAGAGGTGTCCTGCCGGGCTCTGGAGCTCTTCGACTGGCTCGGCGCCGTCTTCAGTAATGTCGAC CTAAATAATGAGCCTAATAATTTCATATCAACCTATTGCTGTCCTGAGCCAAGCACAGTGGTGGCAAAAG CTTATTTGTGTACAATCACTGGCTTCATACTTCCAGAGAAGATCTGTCTCCTATTGGAACATCTCTGTCA CTACTTTGATGAACCGAAGTTAGCTCCATGGGTTACACTGTCCGTTCAAGGCTTTGCAGACAGCCCTGTT TCTTGGGAAAAAAATGAACATGGTTTTCGAAAAGGAGGAGAACATTTATATAACTTTGTGATTTTTAATA ATCAGGACTATTGGCTTCAGATGGCTGTTGGGGCAAATGATCACTGTCCACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237427 representing NM_001286133
Red=Cloning site Green=Tags(s) MATLRRLREAPRHLLVCEKSNFGNHKSRHRHLVQTHYYNYRVSFLIPECGILSEELKNLVMNTGPYYFVK NLPLHELITPEFISTFIKKGSCYALTYNTHIDEDNTVALLPNGKLILSLDKDTYEETGLQGHPSQFSGRK IMKFSSEESTMMSYFSKYQIQEHQPKVALSTLRDLQCPVLQSSELEGTPEVSCRALELFDWLGAVFSNVD LNNEPNNFISTYCCPEPSTVVAKAYLCTITGFILPEKICLLLEHLCHYFDEPKLAPWVTLSVQGFADSPV SWEKNEHGFRKGGEHLYNFVIFNNQDYWLQMAVGANDHCPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001286133 |
ORF Size | 963 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286133.1, NP_001273062.1 |
RefSeq Size | 1074 bp |
RefSeq ORF | 966 bp |
Locus ID | 10799 |
UniProt ID | O75818 |
Protein Families | Stem cell - Pluripotency |
MW | 37.2 kDa |
Gene Summary | Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:9630247, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |