RHBDL1 (NM_001278721) Human Tagged ORF Clone
CAT#: RC237287
- TrueORF®
RHBDL1 (myc-DDK-tagged) - Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1), transcript variant 2
ORF Plasmid: tGFP
"NM_001278721" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RHBDL; RRP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237287 representing NM_001278721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGGAGCTCGCTGCTGCAGCTCATCCAGGAGCAGCAGCTGGACCCCGAGAACACAGGCTTCATCG GTGCGGACACCTTCACTGGCCTGGTGCACAGCCATGAGCTGCCCCTGGACCCGGCCAAGCTGGACATGCT GGTGGCCCTGGCTCAGAGCAACGAGCAGGGCCAGGTCTGCTACCAGGAGCTGGTGGACCTGATCATCGTG TTCCTGTGTTACGGGGCCCGCCTCAACAAGTGGGTGCTGCAGACCTACCACCCCGAGTACATGAAGAGCC CCCTTGTGTACCACCCCGGGCACCGTGCCCGCGCCTGGCGCTTCCTCACCTACATGTTCATGCACGTTGG GCTGGAGCAGCTGGGGTTCAACGCCCTCCTGCAGCTGATGATCGGGGTGCCCCTGGAGATGGTGCACGGC CTGCTCCGCATCAGCCTGCTCTACCTGGCAGGCGTGCTGGCAGGCTCCCTAACCGTCTCCATCACCGACA TGCGGGCCCCGGTGGTGGGAGGCTCCGGCGGGGTCTACGCCCTGTGCTCGGCACACCTGGCCAACGTTGT CATGAACTGGGCTGGGATGAGATGTCCCTACAAGTTGCTGAGGATGGTGCTGGCCTTGGTGTGCATGAGC TCCGAGGTGGGCCGGGCCGTGTGGCTGCGCTTCTCCCCGCCGCTGCCCGCCTCGGGCCCACAGCCCAGCT TCATGGCGCACCTGGCAGGCGCGGTGGTGGGGGTGAGCATGGGCCTGACCATCCTGCGGAGCTACGAGGA GCGCCTGCGGGACCAGTGCGGCTGGTGGGTGGTGCTGCTGGCCTACGGCACCTTCCTGCTCTTCGCCGTC TTCTGGAACGTCTTCGCCTACGACCTGCTGGGCGCCCACATCCCCCCACCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237287 representing NM_001278721
Red=Cloning site Green=Tags(s) MDRSSLLQLIQEQQLDPENTGFIGADTFTGLVHSHELPLDPAKLDMLVALAQSNEQGQVCYQELVDLIIV FLCYGARLNKWVLQTYHPEYMKSPLVYHPGHRARAWRFLTYMFMHVGLEQLGFNALLQLMIGVPLEMVHG LLRISLLYLAGVLAGSLTVSITDMRAPVVGGSGGVYALCSAHLANVVMNWAGMRCPYKLLRMVLALVCMS SEVGRAVWLRFSPPLPASGPQPSFMAHLAGAVVGVSMGLTILRSYEERLRDQCGWWVVLLAYGTFLLFAV FWNVFAYDLLGAHIPPPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278721 |
ORF Size | 894 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001278721.2 |
RefSeq Size | 1255 bp |
RefSeq ORF | 897 bp |
Locus ID | 9028 |
UniProt ID | O75783 |
Protein Families | Protease, Transmembrane |
MW | 33.5 kDa |
Gene Summary | This gene encodes a protein similar to Rhomboid in Drosophila which is involved in signalling in the Spitz/epidermal growth factor receptor/mitogen-activated protein kinase pathway. The Rhomboid family of proteins consists of intramembrane serine proteases containing several transmembrane domains. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
Documents
Product Manuals |
FAQs |
SDS |