HSH2D (NM_001291274) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ALX; HSH2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237259 representing NM_001291274 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGGAGTAGGATGTCCCAGCCCCCGCAGTGTCTCCGCAGAGCCCAAAGCAGCTGCTGCCATTTCA TGGTGAAGCTCTTGGATGATGGGACTTTCATGATCCCCGGGGAGAAGGTGGCCCACACCTCGCTGGACGC CCTGGTCACCTTCCACCAGCAGAAGCCAATTGAGCCGCGCAGGGAGCTGCTGACACAGCCCTGCAGGCAG AAGGATCCCGCAAACGTGGATTACGAGGATCTCTTCCTCTACTCCAACGCAGTGGCCGAGGAAGCTGCCT GCCCGGTGTCTGCCCCTGAGGAGGCCTCCCCAAAGCCAGTCCTGTGTCACCAATCAAAGGAAAGGAAGCC GTCAGCAGAGATGAACAGAATAACCACCAAGGAAGCCACTTCCTCCTGCCCCCCAAAATCCCCTCTTGGA GAGACCCGCCAGAAACTCTGGAGGAGCCTCAAAATGCTCCCCGAGAGAGGCCAGAGGGTCCGGCAGCAGC TAAAAAGCCACCTCGCCACTGTGAACTTGTCGTCACTCTTGGATGTCCGGAGATCCACGGTGATCTCAGG CCCTGGGACCGGAAAAGGCAGCCAAGATCACTCAGGGGATCCCACCTCGGGGGACAGAGGCTACACGGAT CCCTGTGTGGCCACATCTCTCAAAAGCCCCTCACAGCCCCAGGCACCAAAAGACAGAAAGGTCCCCACCA GGAAGGCCGAGAGGTCGGTCAGCTGCATTGAGGTGACCCCAGGGGACAGGAGTTGGCACCAAATGGTAGT GAGAGCCCTATCCTCCCAGGAGTCCAAGCCAGAGCACCAGGGCTTGGCAGAGCCTGAGAACGACCAGCTC CCGGAGGAGTACCAACAACCGCCACCCTTTGCCCCTGGGTACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237259 representing NM_001291274 Red=Cloning site Green=Tags(s) MRGSRMSQPPQCLRRAQSSCCHFMVKLLDDGTFMIPGEKVAHTSLDALVTFHQQKPIEPRRELLTQPCRQ KDPANVDYEDLFLYSNAVAEEAACPVSAPEEASPKPVLCHQSKERKPSAEMNRITTKEATSSCPPKSPLG ETRQKLWRSLKMLPERGQRVRQQLKSHLATVNLSSLLDVRRSTVISGPGTGKGSQDHSGDPTSGDRGYTD PCVATSLKSPSQPQAPKDRKVPTRKAERSVSCIEVTPGDRSWHQMVVRALSSQESKPEHQGLAEPENDQL PEEYQQPPPFAPGYC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291274
ORF Size	885 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291274.1, NP_001278203.1
RefSeq Size	2936 bp
RefSeq ORF	888 bp
Locus ID	84941
UniProt ID	Q96JZ2
MW	33.2 kDa
Gene Summary	T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.