RGS20 (NM_001286673) Human Tagged ORF Clone
CAT#: RC237079
- TrueORF®
RGS20 (myc-DDK-tagged) - Human regulator of G-protein signaling 20 (RGS20), transcript variant 3
ORF Plasmid: tGFP
"NM_001286673" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | g(z)GAP; gz-GAP; RGSZ1; ZGAP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237079 representing NM_001286673
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCAGCTTTCCCAAGATAACCAAGAGTGCCTCCAGAAACATTTCTCCAGGCCGTCTATATGGACAC AGTTTCTGCCCCTGTTCAGGGCTCAGAGATATAATACAGACATTCACCAAATCACAGAAAATGAAGGAGA CCTCAGGGCTGTTCCTGATATCAAGCAGATGGGATCAGAGCGGATGGAGATGCGGAAGCGGCAGATGCCC GCCGCCCAGGACACACCAGGCGCCGCCCCAGGCCAGCCCGGAGCGGGGAGTCGCGGGTCCAACGCATGCT GCTTCTGCTGGTGCTGCTGTTGTAGCTGCTCGTGTCTCACTGTTAGAAACCAGGAAGATCAGAGGCCCAC AATAGCTTCCCACGAACTCAGAGCAGATCTTCCAACCTGGGAAGAAAGCCCTGCTCCTACTCTGGAAGAA GTCAACGCCTGGGCTCAGTCATTTGACAAATTAATGGTCACTCCAGCAGGAAGGAATGCATTCCGTGAAT TCCTCCGAACAGAATTCAGTGAGGAAAATATGCTCTTCTGGATGGCCTGTGAGGAACTGAAAAAGGAAGC TAATAAAAACATTATTGAAGAGAAAGCAAGGATAATCTATGAAGACTACATTTCTATACTTTCTCCTAAG GAGGTGAGCTTAGACTCCCGGGTGAGAGAAGTGATCAACAGAAACATGGTGGAGCCATCCCAACACATAT TCGATGATGCTCAACTTCAGATTTACACCCTGATGCACAGAGACTCATATCCTCGATTCATGAACTCTGC TGTCTATAAGGACTTGCTTCAGTCCTTATCGGAGAAATCTATTGAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237079 representing NM_001286673
Red=Cloning site Green=Tags(s) MPQLSQDNQECLQKHFSRPSIWTQFLPLFRAQRYNTDIHQITENEGDLRAVPDIKQMGSERMEMRKRQMP AAQDTPGAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVRNQEDQRPTIASHELRADLPTWEESPAPTLEE VNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNIIEEKARIIYEDYISILSPK EVSLDSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDSYPRFMNSAVYKDLLQSLSEKSIEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001286673 |
ORF Size | 819 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286673.2 |
RefSeq Size | 1760 bp |
RefSeq ORF | 822 bp |
Locus ID | 8601 |
UniProt ID | O76081 |
Protein Families | Druggable Genome |
MW | 31.9 kDa |
Gene Summary | The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
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