RBM7 (NM_001286047) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC236035 representing NM_001286047 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAACATGACTTCATCAGCACAGATAATTCAGAGATCTTTCTCTTCTCCAGAAAATTTTCAGAGAC AAGCAGTGATGAACAGTGCTTTGAGACAAATGTCATATGGTGGAAAATTTGGTTCTTCACCTCTGGATCA ATCAGGATTTTCACCATCAGTTCAATCACACAGTCATAGTTTCAATCAGTCTTCAAGCTCCCAGTGGCGC CAAGGTACACCATCATCACAGCGTAAAGTCAGAATGAATTCTTATCCCTACCTAGCAGATAGACATTATA GCCGGGAACAGCGTTACACTGATCATGGGTCTGACCATCATTACAGAGGAAAGAGAGATGATTTCTTCTA TGAAGACAGGAATCATGATGACTGGAGCCATGACTATGATAACAGAAGAGACAGTAGTAGAGATGGAAAA TGGCGCTCATCTCGACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC236035 representing NM_001286047 Red=Cloning site Green=Tags(s) MDNMTSSAQIIQRSFSSPENFQRQAVMNSALRQMSYGGKFGSSPLDQSGFSPSVQSHSHSFNQSSSSQWR QGTPSSQRKVRMNSYPYLADRHYSREQRYTDHGSDHHYRGKRDDFFYEDRNHDDWSHDYDNRRDSSRDGK WRSSRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286047
ORF Size	438 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286047.2
RefSeq Size	3587 bp
RefSeq ORF	441 bp
Locus ID	10179
UniProt ID	Q9Y580
MW	17.7 kDa
Gene Summary	Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484). Possible involved in germ cell RNA processing and meiosis (Probable).[UniProtKB/Swiss-Prot Function]

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