PRAX 1 (TSPOAP1) (NM_001261835) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BZRAP1; PBR-IP; PRAX-1; PRAX1; RIM-BP1; RIMBP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235339 representing NM_001261835 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAACTGACAACCCTCCCACGGCCTGGGGACCCTGGAGCCATGGAGCCATGGGCACTGCCCACCT GGCATAGCTGGACTCCAGGTCGAGGGGGTGAACCTAGCAGTGCAGCCCCAAGCATCGCTGATACTCCTCC GGCAGCTCTGCAGCTTCAAGAACTGAGGTCTGAGGAGAGTTCCAAGCCCAAAGGAGACGGGAGCTCCAGG CCCGTGGGGGGAACTGACCCTGAAGGAGCAGAGGCTTGTCTGCCCAGCCTGGGCCAGCAAGCATCCAGCT CTGGACCCGCCTGCCAGAGGCCAGAGGATGAGGAAGTGGAGGCTTTCCTGAAGGCCAAGCTGAATATGAG CTTTGGGGACAGGCCCAATCTGGAGCTGCTGAGGGCCCTGGGGGAGCTGCGGCAGCGCTGTGCCATCCTT AAGGAGGAAAACCAGATGCTGAGGAAGAGCAGCTTCCCTGAGACAGAAGAGAAGGTGCGGAGGCTGAAGA GGAAGAACGCCGAGCTGGCGGTCATTGCCAAGCGCCTGGAGGAGAGGGCCCGAAAGCTGCAGGAAACGAA CCTGAGGGTGGTGAGTGCCCCCTTGCCCCGGCCGGGGACCAGCTTGGAGTTGTGTCGGAAGGCCCTAGCC CGCCAGCGAGCCCGGGACCTCAGTGAGACAGCCAGTGCACTGCTGGCCAAGGACAAGCAGATTGCTGCCT TGCAGCGGGAGTGCAGGGAGCTGCAGGCCAGGCTCACTCTGGTGGGCAAGGAGGGTCCCCAGTGGCTCCA CGTGCGGGACTTCGATCGGCTGCTGCGCGAGTCCCAGCGGGAGGTGCTGCGGCTGCAGAGGCAGATCGCG CTGCGCAACCAGCGGGAGACGCTCCCGCTCCCGCCGTCCTGGCCCCCGGGCCCTGCTCTCCAGGCCAGAG CAGGGGCGCCTGCTCCCGGGGCCCCGGGAGAGGCCACGCCCCAGGAGGATGCGGACAACCTACCCGTGAT TCTAGGGGAGCCAGAGAAAGAGCAGAGGGTGCAGCAGCTGGAATCGGAGCTCAGCAAGAAGCGGAAGAAA TGCGAGAGCCTGGAGCAGGAAGCCCGGAAAAAGCAGAGGCGATGTGAGGAGCTGGAACTGCAGCTGAGAC AAGCGCAGAATGAGAATGCCCGCCTGGTGGAGGAGAACTCCCGGCTCAGTGGGAGAGCCACAGAGAAGGA GCAGGTGGAGTGGGAGAATGCGGAGCTGAGGGGCCAGCTCCTGGGGGTGACACAGGAGAGGGACTCAGCC CTTCGCAAGAGCCAGGGCCTGCAGAGCAAGCTGGAGAGCCTGGAGCAAGTGCTGAAGCACATGCGGGAGG TGGCCCAGCGGCGGCAGCAGCTGGAGGTGGAGCATGAACAGGCTCGGCTCAGCCTACGGGAGAAGCAGGA GGAGGTCCGGAGACTGCAGCAGGCCCAGGCTGAAGCCCAGAGGGAACATGAAGGAGCCGTGCAGCTGCTG GAGTCTACCTTGGATTCCATGCAGGCCCGGGTTCGAGAGCTCGAAGAACAGTGCCGCAGCCAAACCGAGC AGTTCAGCCTCCTGGCACAGGAACTCCAGGCTTTCCGCCTGCACCCGGGCCCCTTGGATCTGCTCACATC TGCCCTGGACTGTGGGAGCCTTGGAGACTGCCCACCACCCCCCTGCTGCTGCTCCATTCCCCAGCCTTGC CGGGGGTCTGGCCCCAAAGACCTTGACCTCCCGCCGGGCTCCCCTGGGCGCTGCACCCCAAAGTCTTCCG AGCCTGCCCCTGCCACTCTCACTGGGGTCCCTCGAAGGACAGCCAAGAAGGCAGAGTCTCTCTCCAACTC CTCCCACTCCGAGTCCATCCACAACAGCCCCAAGTCATGCCCTACACCTGAGGTGGACACAGCCAGTGAG GTAGAGGAGCTGGAGGCAGACAGTGTCTCCCTGCTCCCAGCTGCGCCAGAGGGCAGCCGGGGAGGAGCCA GGATCCAGGTCTTCCTAGCACGTTATAGCTACAACCCCTTTGAGGGTCCCAATGAGAATCCAGAAGCAGA GCTTCCGCTGACAGCTGGCGAGTACATCTACATCTATGGCAACATGGATGAGGATGGCTTTTTTGAAGGA GAGCTCATGGATGGCCGAAGGGGCCTGGTCCCTTCCAATTTTGTAGAGCGTGTGTCGGATGATGACCTCC TGACCTCCCTCCCTCCAGAGCTGGCCGATTTGTCCCACAGCTCAGGCCCTGAACTCAGTTTCCTGAGTGT AGGTGGGGGTGGCAGCAGTAGCGGGGGCCAAAGCAGTGTGGGAAGGAGCCAGCCCAGACCTGAGGAGGAG GATGCAGGGGACGAGCTCAGTCTGAGCCCATCACCGGAGGGCCTGGGCGAGCCTCCTGCCGTGCCTTACC CCCGCCGTCTGGTGGTCCTCAAGCAGCTGGCCCACAGCGTGGTGCTGGCCTGGGAGCCGCCTCCTGAGCA AGTGGAGCTACACGGCTTCCATATCTGTGTGAATGGGGAGCTGCGACAGGCCCTGGGGCCTGGGGCGCCA CCCAAGGCCGTGCTGGAGAACCTGGACCTGTGGGCCGGGCCCCTTCACATTTCTGTCCAGGCCCTGACTA GCCGGGGCAGCTCTGACCCACTGCGCTGTTGCTTGGCGGTGGGTGCCCGGGCCGGAGTGGTGCCCAGCCA GCTGCGGGTCCATCGGTTGACAGCCACATCTGCTGAGATCACCTGGGTGCCCGGCAATAGCAACTTGGCC CATGCCATCTACCTCAATGGGGAAGAGTGCCCACCTGCCAGCCCCAGTACCTACTGGGCCACCTTCTGCC ACTTACGGCCTGGCACACCCTATCAGGCCCAAGTGGAGGCTCAGCTCCCACCCCAAGGGCCCTGGGAACC AGGCTGGGAGAGGCTGGAGCAGCGGGCTGCCACCCTGCAGTTCACCACACTCCCAGCAGGCCCACCTGAT GCCCCTCTGGATGTGCAGATCGAGCCTGGGCCCTCCCCTGGGATCTTGATCATCAGTTGGCTCCCAGTCA CCATCGATGCTGCTGGCACATCCAACGGTGTCCGGGTCACAGGCTATGCCATCTACGCTGATGGGCAGAA GATCATGGAGGTGGCCTCACCCACGGCAGGCAGTGTACTGGTGGAGTTGTCCCAGCTGCAGCTGCTGCAG GTGTGTCGTGAGGTGGTCGTGCGCACCATGTCGCCCCACGGGGAGTCGGCGGACTCCATCCCGGCTCCTA TCACTCCCGCCCTGGCTCCGGCCAGCCTGCCAGCCCGAGTCTCCTGCCCCTCACCGCACCCAAGCCCAGA GGCCAGAGCGCCCCTTGCTTCAGCCTCCCCAGGGCCTGGAGACCCCAGCTCTCCTCTCCAGCACCCTGCT CCCCTTGGAACTCAAGAGCCTCCAGGAGCACCCCCTGCAAGCCCTTCCAGAGAGATGGCAAAAGGGTCCC ACGAGGACCCTCCAGCACCTTGCTCCCAGGAGGAGGCTGGGGCAGCAGTGCTGGGCACCTCAGAGGAGAG GACAGCCAGCACATCTACCCTGGGTGAGAAGGACCCTGGCCCCGCAGCTCCCTCACTGGCCAAGCAGGAG GCCGAGTGGACTGCAGGAGAGGCCTGTCCGGCCTCCAGCTCCACCCAGGGAGCACGGGCCCAGCAGGCGC CAAATACCGAGATGTGCCAAGGAGGAGACCCAGGGTCTGGGCTGAGGCCCAGGGCTGAGAAGGAGGACAC AGCAGAGCTTGGGGTTCATCTGGTGAACTCCCTCGTGGACCACGGCCGCAACTCAGACCTGTCAGACATC CAGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGCTGGGTTCCAGGACTTGCTCCTTCCAGA AGCAGGTTGCTGGCAACAGCATCAGGGAGAATGGGGCCAAGTCCCAGCCCGACCCCTTTTGTGAGACTGA CAGCGATGAGGAGATCTTGGAGCAGATCCTGGAGCTGCCCCTCCAGCAGTTCTGTAGCAAGAAGCTCTTT AGCATCCCGGAGGAGGAGGAAGAGGAAGAGGAGGACGAGGAGGAGGAGAAGTCAGGGGCAGGCTGTTCTT CCCGAGACCCTGGCCCGCCTGAACCTGCATTGCTGGGGCTGGGCTGTGACAGTGGTCAGCCCCGAAGACC TGGCCAGTGTCCCTTGTCTCCTGAGTCCTCCAGGGCTGGAGACTGCCTGGAAGACATGCCTGGATTAGTT GGTGGAAGCAGCCGGAGGAGAGGAGGGGGCTCCCCTGAGAAGCCCCCAAGCCGCAGGCGGCCTCCAGATC CCCGAGAACACTGCAGCCGACTTCTCAGCAACAATGGGCCCCAGGCCTCTGGACGACTGGGCCCCACACG GGAGAGGGGTGGCCTCCCCGTAATTGAGGGCCCCAGGACTGGACTAGAGGCTAGCGGGAGAGGCCGGCTG GGCCCTTCCCGGAGGTGCTCCCGTGGCCGGGCGCTGGAGCCTGGCCTGGCCAGCTGCCTTTCCCCCAAGT GCTTGGAAATCAGCATTGAATATGATTCGGAGGATGAGCAGGAGGCGGGCAGCGGGGGCATCAGCATCAC CAGCTCCTGCTACCCTGGAGATGGGGAGGCCTGGGGCACAGCAACTGTAGGAAGGCCCAGGGGGCCTCCG AAGGCCAATTCAGGCCCCAAGCCCTACCCACGCCTCCCAGCCTGGGAGAAAGGGGAACCAGAGCGGAGAG GCCGCAGTGCGACGGGCAGAGCCAAGGAGCCACTCTCCCGGGCAACAGAGACCGGAGAGGCCAGAGGGCA GGACGGCTCTGGGCGGAGGGGCCCCCAGAAGAGAGGTGTCCGAGTCCTCAGGCCAAGCACTGCAGAGCTA GTCCCTGCGAGGAGCCCCTCAGAAACACTGGCTTACCAGCACCTACCCGTCAGGATCTTTGTGGCTCTGT TTGACTATGACCCCGTGTCAATGTCGCCCAATCCTGATGCTGGAGAAGAAGAGCTTCCCTTCCGAGAGGG TCAGATCCTGAAGGTGTTTGGGGACAAGGATGCCGATGGCTTCTACCAGGGCGAAGGTGGGGGCCGGACA GGCTACATTCCCTGCAACATGGTGGCTGAGGTGGCTGTGGACAGCCCTGCTGGGAGACAGCAACTGCTCC AGCGGGGTTATTTGTCCCCAGATATTCTCCTTGAGGGCTCAGCCCACACAACTGGGCCTCCTCCCAAGCC CCGCCGCTCCAAGAAAGCTGAGTCGGAAGGCCCTGCCCAGCCCTGTCCAGGCCCCCCTAAGCTGGTCCCC TCTGCTGACCTGAAAGCTCCCCACTCCATGGTGGCTGCATTTGACTACAACCCCCAGGAGAGTTCCCCCA ATATGGACGTGGAGGCAGAGCTGCCCTTCCGGGCAGGGGATGTCATTACTGTGTTTGGGGGCATGGACGA TGACGGTTTCTACTATGGGGAATTAAATGGACAAAGGGGCCTGGTTCCATCCAACTTCCTGGAGGGCCCT GGGCCTGAGGCAGGCGGCCTGGACAGGGAACCCAGGACACCCCAGGCTGAGAGTCAGAGAACGAGGAGGA GAAGAGTCCAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235339 representing NM_001261835 Red=Cloning site Green=Tags(s) MEQLTTLPRPGDPGAMEPWALPTWHSWTPGRGGEPSSAAPSIADTPPAALQLQELRSEESSKPKGDGSSR PVGGTDPEGAEACLPSLGQQASSSGPACQRPEDEEVEAFLKAKLNMSFGDRPNLELLRALGELRQRCAIL KEENQMLRKSSFPETEEKVRRLKRKNAELAVIAKRLEERARKLQETNLRVVSAPLPRPGTSLELCRKALA RQRARDLSETASALLAKDKQIAALQRECRELQARLTLVGKEGPQWLHVRDFDRLLRESQREVLRLQRQIA LRNQRETLPLPPSWPPGPALQARAGAPAPGAPGEATPQEDADNLPVILGEPEKEQRVQQLESELSKKRKK CESLEQEARKKQRRCEELELQLRQAQNENARLVEENSRLSGRATEKEQVEWENAELRGQLLGVTQERDSA LRKSQGLQSKLESLEQVLKHMREVAQRRQQLEVEHEQARLSLREKQEEVRRLQQAQAEAQREHEGAVQLL ESTLDSMQARVRELEEQCRSQTEQFSLLAQELQAFRLHPGPLDLLTSALDCGSLGDCPPPPCCCSIPQPC RGSGPKDLDLPPGSPGRCTPKSSEPAPATLTGVPRRTAKKAESLSNSSHSESIHNSPKSCPTPEVDTASE VEELEADSVSLLPAAPEGSRGGARIQVFLARYSYNPFEGPNENPEAELPLTAGEYIYIYGNMDEDGFFEG ELMDGRRGLVPSNFVERVSDDDLLTSLPPELADLSHSSGPELSFLSVGGGGSSSGGQSSVGRSQPRPEEE DAGDELSLSPSPEGLGEPPAVPYPRRLVVLKQLAHSVVLAWEPPPEQVELHGFHICVNGELRQALGPGAP PKAVLENLDLWAGPLHISVQALTSRGSSDPLRCCLAVGARAGVVPSQLRVHRLTATSAEITWVPGNSNLA HAIYLNGEECPPASPSTYWATFCHLRPGTPYQAQVEAQLPPQGPWEPGWERLEQRAATLQFTTLPAGPPD APLDVQIEPGPSPGILIISWLPVTIDAAGTSNGVRVTGYAIYADGQKIMEVASPTAGSVLVELSQLQLLQ VCREVVVRTMSPHGESADSIPAPITPALAPASLPARVSCPSPHPSPEARAPLASASPGPGDPSSPLQHPA PLGTQEPPGAPPASPSREMAKGSHEDPPAPCSQEEAGAAVLGTSEERTASTSTLGEKDPGPAAPSLAKQE AEWTAGEACPASSSTQGARAQQAPNTEMCQGGDPGSGLRPRAEKEDTAELGVHLVNSLVDHGRNSDLSDI QEEEEEEEEEEEEELGSRTCSFQKQVAGNSIRENGAKSQPDPFCETDSDEEILEQILELPLQQFCSKKLF SIPEEEEEEEEDEEEEKSGAGCSSRDPGPPEPALLGLGCDSGQPRRPGQCPLSPESSRAGDCLEDMPGLV GGSSRRRGGGSPEKPPSRRRPPDPREHCSRLLSNNGPQASGRLGPTRERGGLPVIEGPRTGLEASGRGRL GPSRRCSRGRALEPGLASCLSPKCLEISIEYDSEDEQEAGSGGISITSSCYPGDGEAWGTATVGRPRGPP KANSGPKPYPRLPAWEKGEPERRGRSATGRAKEPLSRATETGEARGQDGSGRRGPQKRGVRVLRPSTAEL VPARSPSETLAYQHLPVRIFVALFDYDPVSMSPNPDAGEEELPFREGQILKVFGDKDADGFYQGEGGGRT GYIPCNMVAEVAVDSPAGRQQLLQRGYLSPDILLEGSAHTTGPPPKPRRSKKAESEGPAQPCPGPPKLVP SADLKAPHSMVAAFDYNPQESSPNMDVEAELPFRAGDVITVFGGMDDDGFYYGELNGQRGLVPSNFLEGP GPEAGGLDREPRTPQAESQRTRRRRVQC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001261835
ORF Size	5544 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001261835.2
RefSeq Size	7671 bp
RefSeq ORF	5547 bp
Locus ID	9256
UniProt ID	O95153
Protein Families	Druggable Genome
MW	199.6 kDa

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