KIAA0586 (NM_001244189) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235327 representing NM_001244189 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTGGTGTGGGACTTGTTTTGTGACCAACAATATGAAAGGCTCTGAGGTCAGCTTGGAGAAGAAAA AAAAGATTAAGATGCCAGTGAAGAGACTTCGTGAGGTAGTTTCTCAAAATCATGGAGATCATTTGGTTTT GCTGAAAGATGAGTTGCCCTGTGTTCCTCCGGCATTGTCTGCAAATAAACGTCTTCCTGTTGGAACGGGG ACTAGTTTGAATGGAACATCACGTGGTTCATCAGACTTAACTTCTGCTAGAAATTGTTACCAGCCTCTAT TAGAAAATCCCATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAA AATAGAAGAGAACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGAT GCTCTAAGAACAGTTTTAAAGCAAAATGTCTCACTTTGTCTCACAGGCTGGAGTGATCACAGTGGGGTGA TCACAACTCACTGCAGTCTCTACCTCCTCAGGCTCATGAGATCCTCCCACCTCAGCCTCCCGAGTAGCTG GGACTACAGAGCTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATA GAGAAGGATGCTGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTG CAGCAACTGCTGCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGT CAATTCTGTTACAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAG CAAACAAGCATTCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTA TGGAGCAGCACATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAG TGCTGCACTCAAGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCC GTAAAACCAGAACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAAG CACCTTTAAAAGAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCTCG CAGATTTGCTCCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAAAAA GAAAATATGGAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAATGATT CTTTGACAAGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACTCCTGA GAAAACAAACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCTGATGAT GTTCTTCATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTCTGAGTA TGTTGAAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATCTGTATT GAAAGATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTGGAAGCT ATTATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAGAGATGT CAGAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCAGGATGA ACTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAGAAAGGTCAGAAT ATGACTAAAGATATTAGAACCAACACACAAGATAAAACTGTCAACAAATCTGTAATTCCAAGAAAACATT CTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCTTCAAGTTTACAGAA AGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTAATACAAGATGAAGATTATATGTTACAAGTCTAT GGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTAAAAAAGGACCATATCTCAGATTTAATTCTCCAT CTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGAACGAGTTAAAGGCACTAAGGTAAAGTCAATAAG AACACAGACTGACTTCTATGCAACAAAACCTAAGAAGATGGATTCTAAAATGAAACATTCTGTTCCTGTG TTACCTCATGGCGATCAGCAATATTTGTTCAGCCCAAGTAGAGAAATGCCTACTTTTTCAGGTACATTGG AAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACAAACCCAAAGTAATAGTGATACCATGCCACCTGC TGGAGTGATTGTCAGCAAGCCACACCCTGTAACTGTGACTACTTCTATTCCTCCATCATCTCGAAAAGTA GAAACTGGAGTAAAGAAACCTAACATAGCCATTGTAGAAATGAAGTCAGAAAAAAAGGATCCTCCTCAGC TTACTGTGCAGGTATTACCCAGTGTAGATATTGACAGCATTTCAAATAGTAGTGCTGATGTCCTTTCACC TCTGTCTAGCCCCAAAGAAGCATCTCTTCCTCCTGTGCAAACTTGGATAAAGACTCCAGAAATTATGAAG GTAGATGAAGAAGAGGTGAAGTTTCCAGGAACTAACTTTGATGAAATAATCGATGTCATACAGGAAGAAG AAAAATGTGATGAAATTCCAGACTCTGAACCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGATTCTAC AAAATATAATGGTCCTCCATTTCCGCCAGTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTGGATAAA GTAATTGAGAGAAAAGAAACACTGGAAAATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGTCAAGAA TTATCTCTGGGCTCTTTCCAGTCCAGCAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGAGACAAG TGAACCACTGACTTCTGACATTGTGGAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGT GTTCCTGTGAACTCAAATGTGATTAAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTGTCATGC TGGGTGACAGAGAAGCAAAGAAGCAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAACAAATGA AACATATTTGCCGGCAAGAGTGTGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCACCTTCA TCACCTGCTAAGGAGTGTGTTTTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATGATATGG CTTTTCCTGTGAAAGAAATATGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAATACTCC AACAGTTACCCCTACTACTACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCC ATTGATAAATTGAAGGTATCAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAG AAGAGAACCCTAACTCACCTCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGA AGAACCAGAGAGTATGGATTTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCT GCCGGCACCAAGGCCCCTTCCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGA GTGTTACTGTCACTGAAACTGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGG TCAAAAATTGGCCCCCAAGATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCC AGCACTCTGCATGATGCCGTTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCC ATAAAAAATTTCATGCAGATGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCA AGCAGTCTATCATTCAGAGGACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTA ACAGCGGCAGCAGAGAACATCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGT CTTTGGATCAACAATGTGATCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGA AGATTCATGTGCTAGTCATGGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTT CTTCCCACAACACTTCTGACAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCC AGTACCAACTAAAGCAAAATCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGT TAGAAATAAATCTGATATTGCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACACAAATT GAGCTTAATCCGTACCTCACATGTGTATTTTCAGGTTTGGGGGTACATGTGAAGAAGGTTTCCTGTATAG GTAAACTCGGGCTGTGGAGGTTTGTCATACAGATTATTTCATCACCCAGGTGGGAAAGCAGTGCCACTCT CCGCTTCACAGATGCCCCCTGCCAAGATGTCAGTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCA GTCTCTGAGTCTCAGCACAATGCAGGAGGACATGGAGTCTTCGGGGGCAGATACCTTCTGAACGGGAAGA GACAGCCAGCACAGTGTTTATGCCACTGGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235327 representing NM_001244189 Red=Cloning site Green=Tags(s) MFWCGTCFVTNNMKGSEVSLEKKKKIKMPVKRLREVVSQNHGDHLVLLKDELPCVPPALSANKRLPVGTG TSLNGTSRGSSDLTSARNCYQPLLENPMVSESDFSKDVAVQVLPLDKIEENNKQKANDIFISQYTMGQKD ALRTVLKQNVSLCLTGWSDHSGVITTHCSLYLLRLMRSSHLSLPSSWDYRAQSMPVFKEVKVHLLEDAGI EKDAVTQETRISPSGIDSATTVAAATAAAIATAAPLIKVQSDLEAKVNSVTELLSKLQETDKHLQRVTEQ QTSIQRKQEKLHCHDHEKQMNVFMEQHIRHLEKLQQQQIDIQTHFISAALKTSSFQPVSMPSSRAVEKYS VKPEHPNLGSCNPSLYNTFASKQAPLKEVEDTSFDKQKSPLETPAPRRFAPVPVSRDDELSKRENLLEEK ENMEVSCHRGNVRLLEQILNNNDSLTRKSESSNTTSLTRSKIGWTPEKTNRFPSCEELETTKVTMQKSDD VLHDLGQKEKETNSMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKILRGVQNNKKVLEENLEA IIRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQDELSRTDYEQKRFDQKNQRTKKGQN MTKDIRTNTQDKTVNKSVIPRKHSQKQIEEHFRNLPMRGMPASSLQKERKEGLLKATTVIQDEDYMLQVY GKPVYQGHRSTLKKGPYLRFNSPSPKSRPQRPKVIERVKGTKVKSIRTQTDFYATKPKKMDSKMKHSVPV LPHGDQQYLFSPSREMPTFSGTLEGHLIPMAILLGQTQSNSDTMPPAGVIVSKPHPVTVTTSIPPSSRKV ETGVKKPNIAIVEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKEASLPPVQTWIKTPEIMK VDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPPFPPVASTFQPTADILDK VIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSETSEPLTSDIVEGTSSGALQLFVDAG VPVNSNVIKHFVNEALAETIAVMLGDREAKKQGPVATGVSGDASTNETYLPARVCTPLPTPQPTPPCSPS SPAKECVLVKTPDSSPCDSDHDMAFPVKEICAEKGDDMPAIMLVNTPTVTPTTTPPPAAAVFTPTLSDIS IDKLKVSSPELPKPWGDGDLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMDFPAQPPPPEPVPFMPFP AGTKAPSPSQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKILEDIGLYLTNLNDSLS STLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSEDLENSVGELSEGQRPQL TAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYEDSCASHGPMSLGELELEPNSKLV LPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQVSPGDMDRTQI ELNPYLTCVFSGLGVHVKKVSCIGKLGLWRFVIQIISSPRWESSATLRFTDAPCQDVSDAAVSEPRGLLS VSESQHNAGGHGVFGGRYLLNGKRQPAQCLCHWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244189
ORF Size	4932 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244189.2
RefSeq Size	6043 bp
RefSeq ORF	4935 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	182.5 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016]

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