Liprin alpha 2 (PPFIA2) (NM_001220478) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235191 representing NM_001220478 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTTCTCGGATATGAACACCGTTTCTGGCTCCCCTAAAGTGCATCCTCCTAATGGGACCCGGTTTT ACACTTTTCAAGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACTTCTAGAAAAGGA AGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCATTTGGAGTGCCTT GTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTCCCTCAGGAGTAT CCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTTGGATGAAAAGAT TGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCGAGGGATCCACAGAGTCA GAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTCCAATGGTTCTATAGACT CAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAAAACTATGAAATGGCCCA GATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGGAAGCAGAGACAGCAAGA AAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAGGGAGGCCATGGCACAAA AGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCTCAGAGAGAATCTACCTC CATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTATCCTGCGGCAGATGGAA GAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCAGCAGACCATGAGAAAGG CTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTAACCAAGGCTGAAGAGAG ACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGAAGAATCAAGAACTTCAA AGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGATACGGTTGATAGACTTC TGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCTCTAGAAGAAAAGAATGT TTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATGATAAGGAAAGATTAGCA GAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGGCTCTTTAATTGAACCCA CAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGATCCCTAGTGGACAGCCA GTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGGGTGTGCGAAGAGATGAG CCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGGAGTACTAAGCAGCCACC CTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACAATTTTTAGCTCAATGGA TCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTCAGGAACAATTGGATGCC ATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCGTGCTGAAGAAATTGAAA ATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCAGGTACCTCCATTACTGC CTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAACTCCAAAGCTCACCCCT CGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGATCTGAGGAAACATCGGA GAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAATGTGAAACTTCTCCTCC TCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACAATGATGCTCGAAGTAGT TTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAGCCAAGACTCTCTTCACA AAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAAAAAGAAAAAGCTCGACT TGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGGGGTTAGGCAAACTCGGA ACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGAAGCTCGGAGAAAGGGAT TACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGGTTGGGAATGCCTGCGTG GTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTTTATCTGACACTGAGATC CAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGCAATCCAGGAGATGGTTT CCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTCCTTCAGGCAACGTTTGGGTGACTCATGAAGA AATGGAAAATCTTGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCTGGGCCCAGACCCTGGCT TATGGAGATATGAATCATGAGTGGATTGGAAATGAATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAA GTTACTTTATGGAATGCTTGGTAGATGCAAGAATGTTAGATCACCTAACAAAAAAAGATCTCCGTGTCCA TTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAATATGGAATTATGTGCTTAAAGAGGTTGAAT TATGACAGAAAAGAACTAGAAAGAAGACGGGAAGCAAGCCAACATGAAATAAAAGACGTGTTGGTGTGGA GCAATGACCGAGTTATTCGCTGGATACAAGCAATTGGACTTCGAGAATATGCAAATAATATACTTGAGAG CGGTGTGCATGGCTCACTTATAGCCCTGGATGAAAACTTTGACTACAGCAGCTTAGCTTTATTATTACAG ATTCCAACACAGAACACCCAGGCAAGGCAGATTCTTGAAAGAGAATACAATAACCTCTTGGCCCTGGGAA CTGAAAGGCGACTGGATGAAAGTGATGACAAGAACTTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCC TCCTCGTGAAGTACATGGAATCAGCATGATGCCTGGGTCCTCAGAAACATTACCAGCTGGATTTAGGTTA ACCACAACCTCTGGGCAGTCAAGAAAAATGACAACAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACA ACTCCACTGTTCGCACATACTCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235191 representing NM_001220478 Red=Cloning site Green=Tags(s) MIFSDMNTVSGSPKVHPPNGTRFYTFQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECL VSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKIVALREQNVHIQRKMASSEGSTES EHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQELLEKQNYEMAQMKERLAALSSRVGEVEQEAETAR KDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQRESTSIHDMNDKLENELANKEAILRQME EKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEGQLEEKNQELQ RARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEEKNVLIQESETFRKNLEESLHDKERLA EEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVGSLVDSQSDYRTTKVIRRPRRGRMGVRRDE PKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDIDDDDRETIFSSMDLLSPSGHSDAQTLAMMLQEQLDA INKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHPGTSITASVTASSLASSSPPSGHSTPKLTP RSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPPTPRALRMTHTLPSSYHNDARSS LSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGKKEKARLGQLRGFMETEAAAQESLGLGKLG TQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEI QREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLAAPAKTKESEEGSWAQTLA YGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLN YDRKELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYANNILESGVHGSLIALDENFDYSSLALLLQ IPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGSTWRRQFPPREVHGISMMPGSSETLPAGFRL TTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001220478
ORF Size	3456 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001220478.2, NP_001207407.1
RefSeq Size	5517 bp
RefSeq ORF	3459 bp
Locus ID	8499
UniProt ID	O75334
Protein Families	Druggable Genome
MW	132 kDa
Gene Summary	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein has been shown to bind the calcium/calmodulin-dependent serine protein kinase (MAGUK family) protein (also known as CASK) and proposed to regulate higher-order brain functions in mammals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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