MYBPC1 (NM_001254720) Human Tagged ORF Clone
CAT#: RC235182
- TrueORF®
MYBPC1 (Myc-DDK tagged) - Homo sapiens myosin binding protein C, slow type (MYBPC1), transcript variant 7
ORF Plasmid: tGFP
"NM_001254720" in other vectors (2)
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CNY 11,310.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LCCS4; MYBPCC; MYBPCS; MYOTREM; ssMyBP-C |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235182 representing NM_001254720
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGAACCCACTAAGAAAGAGGAACCAAGTAAAGAGAAGGAGGCCGGAACTACACCAGCAAAAGACT GGACCCTTGTCGAAACTCCTCCTGGGGAGGAACAAGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTT GTTCATTGAAAAACCTCAAGGAGGAACAGTGAAAGTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAG GCTGAAGATCTTCTGAGAAAACCCACTATCAAATGGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAG CCGGGAAGCACCTTCAGCTGAAGGAAACCTTTGAGAGGCACAGTCGGGTGTACACATTTGAGATGCAGAT CATCAAGGCCAAAGATAACTTTGCAGGAAATTACAGATGCGAGGTCACCTATAAGGATAAGTTTGACAGC TGTTCATTTGATCTTGAAGTGCACGAATCTACTGGGACTACTCCAAACATTGACATCAGATCTGCTTTCA AGAGAAGTGGAGAAGGTCAAGAGGATGCAGGAGAACTTGACTTTAGTGGTCTCCTGAAACGTAGGGAGGT GAAGCAGCAGGAGGAAGAACCCCAGGTGGACGTATGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTAC GAGAAGATCGCCTTCCAGTATGGAATCACCGACCTGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCA GAGAGGAGAAGAAGAGCGCAGCTTTTGCAAAAATTCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAG AGTGAGGTTTGTTGTGGAGCTGGCAGATCCAAAGTTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATT CGACCCAGTACCAAATACATCTTTGAACACAAAGGATGCCAGAGAATCCTGTTTATCAATAACTGTCAGA TGACAGATGATTCAGAGTATTATGTGACAGCCGGTGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGA GCCTCCAATTATGGTGACCAAACAGCTGGAAGATACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAA TGTGAGGTGTCTGAAGATGATGCCAATGTAAAATGGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAA AATCAAGATACCGAATTAGAGTTGAGGGTAAAAAACACATCTTGATCATAGAGGGAGCAACAAAGGCTGA TGCTGCAGAATATTCAGTAATGACAACAGGAGGACAATCATCTGCTAAACTTAGTGTTGACTTGAAACCT CTGAAGATTTTGACACCTCTGACTGATCAGACTGTAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAA TCTCTGAAAACATACCAGGAAAATGGACTAAAAATGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGT GGTTCACAAGGGAAGGATCCACAAGTTAGTGATAGCCAATGCCCTCACTGAAGATGAAGGTGATTATGTA TTTGCACCTGATGCCTACAATGTTACTCTGCCTGCCAAAGTTCATGTTATTGATCCTCCTAAGATCATCC TGGATGGTCTTGATGCTGACAACACAGTGACAGTGATTGCAGGAAACAAGCTTCGTCTTGAGATCCCCAT CAGCGGAGAACCACCTCCTAAAGCCATGTGGAGCCGGGGAGATAAGGCTATTATGGAAGGCAGTGGCCGG ATAAGAACAGAATCTTACCCTGATAGCAGCACTCTGGTCATTGATATAGCTGAAAGAGATGACTCTGGTG TTTACCACATCAATCTGAAAAACGAAGCTGGAGAGGCACATGCAAGCATCAAGGTTAAAGTTGTGGACTT CCCTGATCCTCCAGTGGCACCGACTGTGACAGAGGTGGGAGATGACTGGTGTATCATGAACTGGGAGCCT CCTGCCTACGACGGAGGCTCTCCAATCCTAGGATATTTTATTGAGAGGAAGAAGAAACAAAGCTCCAGGT GGATGAGGCTGAATTTTGATCTCTGCAAAGAAACAACTTTTGAGCCCAAGAAGATGATTGAAGGTGTGGC CTATGAGGTCCGCATCTTTGCAGTCAATGCCATTGGCATCTCCAAGCCCAGTATGCCCTCCAGGCCTTTT GTTCCTTTGGCTGTAACAAGCCCTCCTACTCTTCTGACTGTGGACTCTGTCACTGACACGACTGTCACGA TGAGGTGGCGCCCCCCAGACCACATTGGTGCAGCAGGTTTAGATGGCTATGTGCTAGAGTATTGCTTTGA AGGAACTGAGGACTGGATAGTTGCAAACAAAGATCTGATTGACAAGACGAAGTTCACCATCACAGGTCTG CCAACAGATGCAAAGATCTTTGTGCGTGTGAAGGCTGTTAATGCAGCTGGTGCCAGCGAGCCCAAGTACT ATTCTCAGCCCATTCTCGTGAAGGAAATCATAGAACCTCCAAAGATTCGCATTCCAAGACACCTGAAGCA AACCTATATCCGCAGAGTTGGAGAAGCTGTCAATCTGGTTATACCTTTCCAGGGAAAACCAAGACCAGAA TTAACTTGGAAGAAGGATGGTGCAGAAATTGATAAGAATCAAATAAACATTCGCAACTCTGAGACTGATA CAATCATATTTATTAGAAAAGCAGAGAGGAGCCACTCTGGGAAATATGATCTGCAAGTCAAAGTGGACAA ATTCGTGGAGACCGCATCAATTGACATCCAGATCATTGACCGTCCAGGTCCACCCCAAATTGTGAAGATT GAGGATGTCTGGGGAGAAAATGTCGCTCTCACATGGACTCCACCAAAGGATGATGGAAATGCTGCTATCA CAGGCTATACCATTCAGAAGGCTGACAAGAAGAGCATGGAATGGTTTACTGTCATTGAGCATTATCATCG AACCAGTGCCACCATTACTGAATTGGTCATAGGGAATGAATATTACTTCCGGGTCTTTTCTGAAAACATG TGTGGCCTCAGTGAGGATGCCACCATGACTAAAGAGAGTGCAGTGATCGCCAGGGATGGTAAAATCTACA AAAATCCAGTGTATGAAGACTTTGATTTCTCAGAGGCACCCATGTTTACTCAGCCTTTGGTTAACACCTA TGCCATAGCTGGTTACAATGCCACCCTAAACTGCAGTGTGAGAGGAAATCCTAAGCCTAAAATAACCTGG ATGAAAAACAAAGTTGCTATTGTGGATGATCCAAGATACAGGATGTTCAGCAACCAGGGAGTCTGTACCC TGGAAATTCGCAAGCCCAGCCCCTATGATGGAGGCACTTACTGCTGCAAAGCAGTCAATGACCTTGGGAC AGTGGAGATTGAATGCAAACTGGAGGTGAAAGTGATATATCAAGGAGTAAATACCCCTGGACAACCAGTC TTCCTGGAGGGGCAGCAACAGTCATTGCACAATAAGGATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235182 representing NM_001254720
Red=Cloning site Green=Tags(s) MPEPTKKEEPSKEKEAGTTPAKDWTLVETPPGEEQAKQNANSQLSILFIEKPQGGTVKVGEDITFIAKVK AEDLLRKPTIKWFKGKWMDLASKAGKHLQLKETFERHSRVYTFEMQIIKAKDNFAGNYRCEVTYKDKFDS CSFDLEVHESTGTTPNIDIRSAFKRSGEGQEDAGELDFSGLLKRREVKQQEEEPQVDVWELLKNAKPSEY EKIAFQYGITDLRGMLKRLKRMRREEKKSAAFAKILDPAYQVDKGGRVRFVVELADPKLEVKWYKNGQEI RPSTKYIFEHKGCQRILFINNCQMTDDSEYYVTAGDEKCSTELFVREPPIMVTKQLEDTTAYCGERVELE CEVSEDDANVKWFKNGEEIIPGPKSRYRIRVEGKKHILIIEGATKADAAEYSVMTTGGQSSAKLSVDLKP LKILTPLTDQTVNLGKEICLKCEISENIPGKWTKNGLPVQESDRLKVVHKGRIHKLVIANALTEDEGDYV FAPDAYNVTLPAKVHVIDPPKIILDGLDADNTVTVIAGNKLRLEIPISGEPPPKAMWSRGDKAIMEGSGR IRTESYPDSSTLVIDIAERDDSGVYHINLKNEAGEAHASIKVKVVDFPDPPVAPTVTEVGDDWCIMNWEP PAYDGGSPILGYFIERKKKQSSRWMRLNFDLCKETTFEPKKMIEGVAYEVRIFAVNAIGISKPSMPSRPF VPLAVTSPPTLLTVDSVTDTTVTMRWRPPDHIGAAGLDGYVLEYCFEGTEDWIVANKDLIDKTKFTITGL PTDAKIFVRVKAVNAAGASEPKYYSQPILVKEIIEPPKIRIPRHLKQTYIRRVGEAVNLVIPFQGKPRPE LTWKKDGAEIDKNQINIRNSETDTIIFIRKAERSHSGKYDLQVKVDKFVETASIDIQIIDRPGPPQIVKI EDVWGENVALTWTPPKDDGNAAITGYTIQKADKKSMEWFTVIEHYHRTSATITELVIGNEYYFRVFSENM CGLSEDATMTKESAVIARDGKIYKNPVYEDFDFSEAPMFTQPLVNTYAIAGYNATLNCSVRGNPKPKITW MKNKVAIVDDPRYRMFSNQGVCTLEIRKPSPYDGGTYCCKAVNDLGTVEIECKLEVKVIYQGVNTPGQPV FLEGQQQSLHNKDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001254720 |
ORF Size | 3402 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001254720.1, NP_001241649.1 |
RefSeq Size | 3883 bp |
RefSeq ORF | 3405 bp |
Locus ID | 4604 |
UniProt ID | Q00872 |
MW | 128.3 kDa |
Gene Summary | This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |