DAAM2 (NM_001201427) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	dJ90A20A.1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235150 representing NM_001201427 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCCGCAAGAGGAGCCACCATGGCCTGGGCTTCCTGTGCTGCTTCGGGGGCAGTGACATCCCCG AAATCAACCTCCGGGACAACCACCCTCTGCAGTTCATGGAGTTCTCCAGCCCCATCCCGAACGCAGAGGA GCTCAACATCCGCTTTGCAGAGCTGGTGGATGAATTGGATCTCACTGACAAAAACCGAGAGGCTATGTTT GCACTGCCCCCTGAGAAGAAATGGCAGATCTACTGCAGCAAGAAGAAGGAGCAGGAGGACCCCAACAAGC TGGCAACCAGCTGGCCTGACTATTACATCGACCGCATCAATTCCATGGCTGCGATGCAGAGTCTGTACGC GTTTGATGAGGAGGAGACGGAGATGAGGAACCAAGTCGTGGAAGACCTGAAGACAGCCCTCCGGACACAG CCTATGAGGTTTGTGACCCGCTTCATTGAGCTGGAGGGCTTGACCTGTCTGCTAAATTTCCTCCGGAGCA TGGACCACGCCACCTGTGAGAGCCGCATCCACACCTCACTCATTGGCTGCATCAAAGCATTGATGAACAA CTCCCAGGGGCGGGCACATGTGCTGGCACAGCCTGAGGCCATTAGTACCATAGCCCAGAGCCTACGCACA GAGAACAGCAAGACCAAGGTGGCTGTGCTGGAGATCCTGGGTGCTGTGTGCCTCGTGCCTGGTGGCCACA AGAAGGTGCTGCAGGCCATGCTGCACTACCAGGTGTATGCAGCAGAGCGAACCCGCTTCCAGACCCTGCT GAACGAGCTAGACCGAAGTCTGGGCCGGTACCGGGATGAAGTGAATCTGAAAACAGCCATCATGTCCTTC ATCAATGCTGTCCTCAATGCTGGAGCTGGAGAGGATAATCTGGAGTTCCGCCTACATCTACGGTATGAAT TCCTGATGCTGGGTATACAGCCTGTGATTGACAAGCTCCGGCAACATGAAAATGCCATCCTGGACAAACA TTTAGACTTCTTCGAGATGGTGCGGAATGAGGATGACCTGGAGCTAGCCAGGAGGTTTGACATGGTCCAC ATCGACACCAAGAGTGCTTCCCAGATGTTTGAGTTGATCCACAAGAAGCTGAAGTACACGGAGGCCTACC CCTGCCTGCTCTCTGTGCTGCACCACTGCCTGCAGATGCCCTACAAACGGAACGGTGGCTACTTCCAGCA GTGGCAGCTCCTGGACCGCATCCTCCAGCAGATTGTCCTCCAGGATGAGCGGGGTGTGGACCCTGACCTG GCTCCCTTGGAGAACTTCAATGTCAAGAACATCGTCAACATGCTCATCAACGAGAATGAAGTGAAACAGT GGCGAGACCAGGCAGAGAAGTTCCGGAAAGAACACATGGAGCTTGTGAGCCGTCTGGAGAGGAAGGAGCG GGAATGCGAGACAAAGACATTGGAGAAGGAAGAGATGATGCGGACGCTGAACAAAATGAAGGACAAGCTG GCCCGGGAGTCCCAGGAGCTGCGCCAGGCTCGGGGACAAGTGGCAGAGCTGGTAGCCCAGCTCAGTGAAC TCTCAACAGGCCCTGTATCTTCCCCACCACCCCCTGGGGGCCCACTCACCTTGTCTTCCTCAATGACAAC CAATGACCTGCCTCCACCCCCTCCTCCTCTGCCCTTTGCCTGTTGTCCCCCTCCCCCACCACCACCCCTT CCTCCCGGGGGACCCCCGACTCCCCCAGGTGCCCCACCTTGCCTCGGCATGGGCCTGCCCCTCCCTCAGG ACCCCTACCCCAGCAGTGACGTCCCACTCAGGAAAAAGCGTGTCCCCCAGCCTTCTCACCCACTGAAGTC CTTCAACTGGGTGAAGCTGAATGAGGAGCGTGTCCCTGGCACCGTATGGAATGAGATTGATGACATGCAG GTATTTCGGATCCTGGACCTAGAGGATTTTGAAAAGATGTTTTCAGCCTACCAGAGGCACCAGAAAGAGC TGGGCTCCACTGAAGACATCTACCTGGCTTCCCGCAAGGTCAAAGAGCTGTCGGTCATTGATGGCCGGAG GGCCCAAAACTGCATCATCCTTCTTTCCAAGTTGAAGCTTTCTAACGAGGAGATCCGGCAGGCCATCTTG AAGATGGATGAGCAGGAGGACCTTGCTAAGGACATGCTGGAGCAGCTCCTCAAGTTCATCCCAGAGAAGA GTGACATTGACCTCCTGGAGGAGCACAAGCATGAAATTGAGCGGATGGCCCGTGCTGACCGCTTCCTCTA TGAAATGAGCAGGATTGACCACTACCAGCAGCGACTGCAAGCCCTCTTCTTCAAGAAGAAATTCCAGGAG CGGCTGGCTGAGGCAAAGCCCAAAGTGGAAGCCATCCTGTTGGCCTCCCGGGAGCTGGTCCGCAGCAAGC GTCTTAGACAGATGCTAGAGGTCATCCTAGCCATAGGCAACTTCATGAACAAAGGGCAGCGTGGGGGCGC CTACGGGTTCCGGGTGGCCAGCCTCAACAAGATCGCTGACACCAAGTCCAGCATCGACAGAAACATCTCT CTGCTCCATTACCTGATCATGATCCTGGAGAAGCATTTTCCTGATATTCTAAACATGCCTTCAGAGCTGC AACATCTTCCAGAAGCTGCCAAAGTCAACCTAGCAGAACTGGAGAAGGAGGTGGGCAACCTCAGGAGGGG CCTGAGAGCGGTGGAGGTGGAGCTGGAGTATCAGAGGCGCCAGGTACGGGAGCCCAGTGACAAGTTTGTC CCTGTCATGAGCGACTTCATCACGGTGTCCAGCTTCAGCTTCTCCGAGCTGGAGGACCAGCTAAATGAGG CCAGGGACAAGTTCGCCAAGGCCTTGATGCACTTCGGGGAGCATGACAGCAAGATGCAGCCAGACGAATT CTTTGGCATCTTTGATACCTTCTTGCAGGCCTTCTCAGAGGCCCGGCAGGATCTAGAGGCCATGAGGAGG AGGAAGGAGGAGGAGGAGCGGCGGGCGCGCATGGAAGCCATGCTGAAGGAGCAGAGGGAACGTGAGCGGT GGCAGCGGCAGCGGAAGGTCCTGGCTGCAGGCAGCTCGCTGGAGGAGGGAGGAGAGTTCGATGACCTGGT GTCGGCCCTGCGCTCTGGGGAGGTCTTCGACAAGGACTTATGCAAGCTCAAGCGCAGCCGCAAGCGATCA GGGAGCCAGGCCCTGGAAGTTACCCGGGAGCGGGCAATAAACCGGCTAAATTAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235150 representing NM_001201427 Red=Cloning site Green=Tags(s) MAPRKRSHHGLGFLCCFGGSDIPEINLRDNHPLQFMEFSSPIPNAEELNIRFAELVDELDLTDKNREAMF ALPPEKKWQIYCSKKKEQEDPNKLATSWPDYYIDRINSMAAMQSLYAFDEEETEMRNQVVEDLKTALRTQ PMRFVTRFIELEGLTCLLNFLRSMDHATCESRIHTSLIGCIKALMNNSQGRAHVLAQPEAISTIAQSLRT ENSKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQVYAAERTRFQTLLNELDRSLGRYRDEVNLKTAIMSF INAVLNAGAGEDNLEFRLHLRYEFLMLGIQPVIDKLRQHENAILDKHLDFFEMVRNEDDLELARRFDMVH IDTKSASQMFELIHKKLKYTEAYPCLLSVLHHCLQMPYKRNGGYFQQWQLLDRILQQIVLQDERGVDPDL APLENFNVKNIVNMLINENEVKQWRDQAEKFRKEHMELVSRLERKERECETKTLEKEEMMRTLNKMKDKL ARESQELRQARGQVAELVAQLSELSTGPVSSPPPPGGPLTLSSSMTTNDLPPPPPPLPFACCPPPPPPPL PPGGPPTPPGAPPCLGMGLPLPQDPYPSSDVPLRKKRVPQPSHPLKSFNWVKLNEERVPGTVWNEIDDMQ VFRILDLEDFEKMFSAYQRHQKELGSTEDIYLASRKVKELSVIDGRRAQNCIILLSKLKLSNEEIRQAIL KMDEQEDLAKDMLEQLLKFIPEKSDIDLLEEHKHEIERMARADRFLYEMSRIDHYQQRLQALFFKKKFQE RLAEAKPKVEAILLASRELVRSKRLRQMLEVILAIGNFMNKGQRGGAYGFRVASLNKIADTKSSIDRNIS LLHYLIMILEKHFPDILNMPSELQHLPEAAKVNLAELEKEVGNLRRGLRAVEVELEYQRRQVREPSDKFV PVMSDFITVSSFSFSELEDQLNEARDKFAKALMHFGEHDSKMQPDEFFGIFDTFLQAFSEARQDLEAMRR RKEEEERRARMEAMLKEQRERERWQRQRKVLAAGSSLEEGGEFDDLVSALRSGEVFDKDLCKLKRSRKRS GSQALEVTRERAINRLNY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001201427
ORF Size	3204 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001201427.2
RefSeq Size	6191 bp
RefSeq ORF	3207 bp
Locus ID	23500
UniProt ID	Q86T65
Protein Pathways	Wnt signaling pathway
MW	123.9 kDa
Gene Summary	Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.