SFRS8 (SFSWAP) (NM_001261411) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SFRS8; SWAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235117 representing NM_001261411 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGGCGCGAGCGGGGGCCGCGCCAAACCCGAGAGGAAAAGCGGCGCGAAGGAGGAGGCCGGGCCAG GCGGTGCCGGCGGTGGGGGCAGCCGAGTGGAGCTCTTGGTTTTCGGCTATGCCTGCAAGCTGTTCCGGGA CGACGAGCGGGCCCTGGCTCAGGAACAGGGACAGCACCTCATCCCCTGGATGGGGGACCACAAGATCCTC ATCGACAGATATGATGGACGTGGTCACCTGCATGACCTTTCTGAGTACGATGCTGAGTATTCCACGTGGA ACAGAGATTATCAGCTGTCTGAAGAGGAGGCGCGAATAGAGGCCCTGTGTGATGAAGAGAGGTATTTAGC CTTGCATACGGACTTGCTTGAGGAGGAGGCAAGGCAAGAGGAAGAATACAAGCGATTGAGTGAAGCACTA GCAGAGGATGGGAGCTACAATGCCGTGGGGTTCACTTACGGTAGCGACTATTACGACCCGTCAGAGCCGA CGGAGGAGGAGGAGCCTTCCAAACAGAGAGAAAAAAATGAGGCCGAAAATTTAGAGGAAAATGAAGAGCC CTTCGTTGCCCCCTTAGGATTGAGCGTCCCGTCTGACGTGGAGTTGCCACCAACCGCTAAAATGCACGCC ATCATCGAGCGCACGGCCAGCTTCGTGTGCAGGCAGGGAGCACAGTTTGAGATCATGCTGAAGGCCAAGC AGGCCCGGAACTCCCAGTTTGACTTTCTGCGCTTCGACCACTACCTCAACCCCTACTATAAGTTCATCCA GAAAGCCATGAAAGAGGGACGCTACACTGTCCTGGCAGAAAACAAAAGTGACGAGAAAAAAAAATCAGGA GTCAGCTCTGACAATGAAGATGATGATGATGAAGAAGATGGGAATTACCTTCATCCCTCTCTCTTTGCCT CCAAGAAGTGTAACCGCCTTGAAGAGCTGATGAAGCCCTTGAAGGTAGTGGACCCAGATCATCCCCTCGC AGCACTTGTTCGTAAGGCACAGGCTGACAGTTCCACTCCCACCCCACACAACGCAGACGGTGCGCCTGTG CAGCCCTCCCAGGTGGAGTACACGGCAGACTCGACCGTGGCAGCCATGTATTACAGCTACTACATGCTAC CGGACGGCACTTACTGCCTGGCGCCGCCCCCTCCCGGAATCGACGTGACTACTTACTACAGCACCCTTCC TGCTGGCGTGACCGTGTCTAACTCCCCTGGAGTGACGACCACCGCCCCACCACCTCCTGGGACCACACCA CTACCGCCCCCAACCACAGCAGAGACTAGCAGCGGGGCCACCTCCACAACCACCACCACAAGTGCACTTG CCCCCGTGGCCGCCATCATCCCCCCGCCCCCCGACGTCCAGCCCGTGATTGACAAGCTGGCCGAGTATGT CGCCAGGAACGGCCTGAAGTTCGAGACCAGTGTTCGTGCCAAGAATGATCAAAGATTTGAGTTCCTGCAG CCGTGGCACCAGTATAATGCTTATTATGAGTTTAAGAAGCAGTTCTTCCTCCAGAAAGAAGGGGGCGATA GCATGCAGGCTGTGTCTGCACCAGAAGAGGCTCCCACAGACTCTGCTCCCGAGAAGCCAAGTGATGCTGG GGAGGATGGCGCGCCTGAAGACGCAGCCGAGGTGGGAGCACGGGCAGGCTCAGGCGGGAAGAAGGAGGCA TCGTCCAGTAAGACCGTCCCGGACGGGAAGCTGGTGAAAGCTTCCTTTGCTCCAATAAGCTTTGCAATCA AGGCCAAAGAAAATGATCTGCTTCCCCTGGAAAAAAATCGTGTTAAGCTAGATGATGACAGTGATGATGA TGAAGAAAGCAAAGAAGGCCAAGAAAGTTCTAGTAGTGCTGCAAACACTAACCCAGCAGTTGCCCCACCC TGTGTAGTTGTTGAGGAGAAGAAGCCTCAACTTACCCAGGAGGAGCTAGAAGCAAAGCAAGCAAAGCAAA AGCTGGAAGATCGCCTCGCAGCTGCTGCCCGGGAAAAGCTGGCCCAGGCGTCTAAGGAGTCAAAAGAGAA ACAGCTTCAAGCAGAACGTAAAAGGAAAGCGGCGTTATTTTTACAGACCCTCAAAAATCCTCTGCCGGAA GCAGAAGCTGGGAAAATTGAGGAGAGTCCTTTCAGTGTCGAGGAATCCAGCACTACGCCCTGCCCTCTAC TGACTGGAGGCAGGCCTCTGCCTACTTTAGAAGTTAAACCACCCGATAGGCCTTCGAGCAAAAGCAAAGA TCCACCGAGAGAAGAAGAGAAAGAAAAGAAAAAGAAAAAGCACAAAAAAAGATCTCGAACAAGATCACGT TCTCCCAAGTACCATTCGTCATCCAAGTCCAGGTCTAGATCACACTCAAAAGCAAAGCATTCTCTTCCCA GTGCCTATCGGACAGTGCGGCGGTCGAGAGTCACAGCCTCCCCAGGGACGCTGCGCGCGGAGCCCTGTCA GAGCAGCGCGTCAGTGACAGCGGCAGCCGAGCCAGGAAGTTATCAGGCAGCCTCGACCACCACCAGATTT GACTCCGCGAGCTCTTTTGAGGGAAAACCTGGTAAAACGTCAAGGTCCCGCTCCCGGTCCCCTCGGAGGA GAGCCCACTCCCCTGAGAGACGGAGGGAAGAGAGGAGTGTGCCCACTGCCTACCGCGTGAGCCGCAGCCC TGGGGCCAGCAGGAAGCGGACCCGCTCCAGAAGTCCCCACGAGAAGAAGAAGAAGAGGCGGTCCCGGTCG CGGACCAAGTCCAAGGCCAGGTCTCAGTCGGTGTCACCCAGCAAGCAGGCAGCGCCCCGGCCCGCGGCCC CCGCGGCCCACTCGGCGCACTCAGCCAGCGTCTCCCCTGTGGAGAGTCGGGGCTCCAGCCAGGAGCGCTC CAGGGGAGTCTCTCAGGAAAAAGAAGCCCAGATCTCTTCAGCAATCGTTTCTTCCGTGCAGAGCAAAATC ACTCAGGATCTCATGGCCAAAGTCAGAGCGATGCTTGCAGCTTCCAAAAACCTGCAAACCAGCGCTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235117 representing NM_001261411 Red=Cloning site Green=Tags(s) MYGASGGRAKPERKSGAKEEAGPGGAGGGGSRVELLVFGYACKLFRDDERALAQEQGQHLIPWMGDHKIL IDRYDGRGHLHDLSEYDAEYSTWNRDYQLSEEEARIEALCDEERYLALHTDLLEEEARQEEEYKRLSEAL AEDGSYNAVGFTYGSDYYDPSEPTEEEEPSKQREKNEAENLEENEEPFVAPLGLSVPSDVELPPTAKMHA IIERTASFVCRQGAQFEIMLKAKQARNSQFDFLRFDHYLNPYYKFIQKAMKEGRYTVLAENKSDEKKKSG VSSDNEDDDDEEDGNYLHPSLFASKKCNRLEELMKPLKVVDPDHPLAALVRKAQADSSTPTPHNADGAPV QPSQVEYTADSTVAAMYYSYYMLPDGTYCLAPPPPGIDVTTYYSTLPAGVTVSNSPGVTTTAPPPPGTTP LPPPTTAETSSGATSTTTTTSALAPVAAIIPPPPDVQPVIDKLAEYVARNGLKFETSVRAKNDQRFEFLQ PWHQYNAYYEFKKQFFLQKEGGDSMQAVSAPEEAPTDSAPEKPSDAGEDGAPEDAAEVGARAGSGGKKEA SSSKTVPDGKLVKASFAPISFAIKAKENDLLPLEKNRVKLDDDSDDDEESKEGQESSSSAANTNPAVAPP CVVVEEKKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQTLKNPLPE AEAGKIEESPFSVEESSTTPCPLLTGGRPLPTLEVKPPDRPSSKSKDPPREEEKEKKKKKHKKRSRTRSR SPKYHSSSKSRSRSHSKAKHSLPSAYRTVRRSRVTASPGTLRAEPCQSSASVTAAAEPGSYQAASTTTRF DSASSFEGKPGKTSRSRSRSPRRRAHSPERRREERSVPTAYRVSRSPGASRKRTRSRSPHEKKKKRRSRS RTKSKARSQSVSPSKQAAPRPAAPAAHSAHSASVSPVESRGSSQERSRGVSQEKEAQISSAIVSSVQSKI TQDLMAKVRAMLAASKNLQTSAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001261411
ORF Size	3009 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001261411.1, NP_001248340.1
RefSeq Size	3414 bp
RefSeq ORF	3012 bp
Locus ID	6433
UniProt ID	Q12872
Protein Families	Transcription Factors
MW	110.5 kDa
Gene Summary	This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.