Lipin 1 (LPIN1) (NM_001261428) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235095 representing NM_001261428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAACAGGACGGCATTCGCAGCTCCAGCTGGGAGACCTCGCAGGGCAAGAGCTCCCCAGACTCGG CTTGGTCATGGATTCCAATAATGAGAGACCCTGGGTGGATTCGAAATGTATGGTCTTCAAACATTAACGT GCAGACCATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGCTCTACAAGGGG CTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGGAAACCTCCAAT GCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAAGTGGTTGACATAGA AATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGGAGATAATGGAGAAGCATTTTTTGTTCAAGAA ACAGATAATGATCAGGAAGTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGA GAATGGAATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCCCA AGTGATCGCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAAG TCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGATGAGGACATGTTCCCCATCG AGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCTTCCTAATGATATACCTCCATT CCAAGATGATATTCCTGAGGAAAACCTCTCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAAT TCGGATAGAGAGTGGTCACCCACTCCCAGTAGCCTGGTAGATTGCAAAAGGACTGCCCCTCATCTTGCAG TTGCGGCCGAGGGAGGTCTGTCTAGTTCTTGCCCTCCACAGTCTTCCCTGTTCCATCCTTCGGAAAGTCC TTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGTCAGCAAGTCCACGGAAAGGACAGGG CAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAGCTGCCGCAGGCTGCTAAGTCTTCTTCTCCACACA AGATGAAAGAGTCCAGCCCATTGAGCAGTAGAAAAATTTGTGATAAAAGTCACTTTCAGGCCATTCACAG CGAATCTTCAGACACTTTTAGTGACCAATCGCCAACTCTGGTCGGTGGGGCACTTTTGGACCAGAACAAG CCTCAGACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAGACCTTAGGAGCAGCAGCGCCACTCTTGC CCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTCCAGACAGCAAACAAGACGGATTCTCCTTC CAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGTCTACTTGGATGACCTCACAGACATG GATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAACGGAGATCCTTCCGGACTCGCAAAACATGCAAGCG ACAACGGAGCCCGGTCAGCCAACCAGTCCCCGCAGTCGGTGGGCAGCTCGGGCGTGGACAGTGGCGTGGA GAGCACCTCGGACGGGCTGAGGGACCTCCCTTCCATCGCCATCTCCCTCTGCGGGGGCCTCAGCGACCAC CGGGAGATCACGAAAGATGCATTCCTGGAGCAAGCTGTGTCATATCAACAGTTTGTGGACAACCCCGCTA TTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATTATAACTGGACAACAGCAGCACCCCT CCTCCTGGCAATGCAGGCCTTCCAGAAACCTTTGCCAAAGGCCACTGTGGAATCTATCATGAGGGATAAA ATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCATGGAGGGGAAGAAACACCACAATCAAGGAGGAAAGTA AGCCAGAGCAGTGCTTGGCTGGCAAGGCCCATAGCACCGGAGAGCAACCGCCGCAGCTCAGCTTGGCCAC CAGGGTAAAGCATGAATCATCCTCCAGTGATGAGGAGCGCGCAGCTGCCAAGCCATCAAACGCAGGCCAC CTCCCTCTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTGACTTCCGAGCAGCTTAAAAGCTTGA AGTTGAAGAATGGCCCCAACGACGTGGTTTTCAGTGTCACCACGCAGTACCAAGGCACGTGCCGCTGTGA GGGCACCATCTATCTGTGGAACTGGGATGATAAAGTCATCATTTCTGATATTGATGGGACAATTACCAGA TCAGATACTCTTGGCCACATTTTGCCCACCCTTGGGAAGGATTGGACCCATCAGGGCATCGCTAAGCTGT ACCATAAAGTGAGCCAGAATGGATATAAATTTCTCTACTGTTCTGCCCGTGCCATCGGGATGGCGGACAT GACGCGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGTGCTGCCCCAGGGGCCCCTGCTGCTGAGT CCCAGCAGCCTCTTCTCTGCCCTGCACAGAGAAGTGATTGAAAAGAAGCCAGAAAAGTTTAAAGTCCAGT GTTTGACAGACATCAAAAACCTGTTTTTCCCCAACACAGAACCCTTTTATGCTGCTTTTGGAAACCGACC AGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTTGAATAGAATATTTACCGTCAACCCTAAAGGA GAGCTGGTACAGGAACATGCAAAGACCAACATCTCTTCGTATGTGAGACTCTGTGAAGTAGTCGACCACG TTTTCCCGTTGCTGAAAAGAAGCCATTCTTCAGACTTTCCCTGTTCGGATACCTTCAGTAACTTCACCTT TTGGAGAGAGCCACTGCCACCTTTTGAAAACCAGGACATTCATTCTGCCTCAGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235095 representing NM_001261428 Red=Cloning site Green=Tags(s) MGEQDGIRSSSWETSQGKSSPDSAWSWIPIMRDPGWIRNVWSSNINVQTMNYVGQLAGQVFVTVKELYKG LNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQE TDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRK SQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSLAVIYPQSASYPN SDREWSPTPSSLVDCKRTAPHLAVAAEGGLSSSCPPQSSLFHPSESPSGSRPSTPKSDSELVSKSTERTG QKNPEMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNK PQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDM DPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDH REITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDK MPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGH LPLLPNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITR SDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLS PSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKG ELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001261428
ORF Size	2925 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001261428.3
RefSeq Size	5620 bp
RefSeq ORF	2928 bp
Locus ID	23175
UniProt ID	Q14693
MW	108.3 kDa
Gene Summary	This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]

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