RBM12 (NM_001198840) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HRIHFB2091; SCZD19; SWAN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235053 representing NM_001198840. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGTGGTCATCCGTTTGCAAGGTCTCCCAATTGTGGCGGGGACCATGGACATTCGCCACTTCTTC TCTGGATTGACCATTCCTGATGGGGGCGTGCATATTGTAGGGGGTGAACTGGGTGAGGCTTTCATCGTT TTTGCCACTGATGAAGATGCAAGGCTTGGTATGATGCGCACAGGTGGTACAATTAAAGGGTCAAAAGTA ACACTATTGTTGAGTAGTAAGACGGAAATGCAGAATATGATTGAACTGAGTCGTAGGCGTTTTGAAACT GCCAACTTAGATATACCACCAGCAAATGCCAGTAGATCAGGACCACCACCTAGCTCAGGAATGAGTAGC AGGGTAAACTTGCCCACAACAGTATCCAACTTTAATAATCCATCACCCAGTGTAGTTACTGCCACCACT TCTGTTCATGAAAGCAACAAAAACATACAGACATTTTCCACAGCCAGCGTAGGAACAGCTCCTCCAAAT ATGGGGGCTTCCTTTGGGAGCCCAACGTTTAGCTCAACTGTTCCAAGCACAGCCTCTCCAATGAACACA GTCCCGCCGCCACCAATTCCTCCAATTCCAGCGATGCCATCTCTGCCACCAATGCCATCCATTCCCCCA ATTCCAGTTCCTCCTCCAGTACCTACATTGCCTCCTGTGCCTCCTGTGCCCCCGATTCCCCCAGTTCCT TCTGTGCCACCCATGACCCCACTGCCACCCATGTCGGGCATGCCGCCCTTGAATCCGCCACCTGTGGCA CCTCTACCTGCTGGAATGAATGGCTCTGGAGCACCTATGAATTTGAACAATAATCTGAATCCTATGTTT CTTGGTCCGTTGAATCCTGTTAACCCTATCCAGATGAACTCTCAGAGCAGTGTGAAGCCACTCCCCATC AACCCTGATGATCTGTATGTCAGTGTGCATGGAATGCCCTTTTCTGCAATGGAAAATGATGTCAGAGAT TTTTTTCATGGGCTCCGTGTTGATGCAGTGCATTTGTTGAAAGATCATGTAGGTCGAAATAATGGGAAT GGATTGGTTAAGTTTCTCTCCCCTCAAGATACATTTGAAGCTTTGAAACGAAACAGAATGCTGATGATT CAACGCTATGTGGAAGTTAGCCCTGCCACAGAAAGACAGTGGGTAGCTGCTGGAGGCCATATCACTTTT AAGCAAAATATGGGACCTTCTGGACAAACTCATCCCCCTCCTCAGACACTTCCCAGGTCAAAATCGCCC AGTGGGCAGAAAAGATCAAGGTCAAGATCACCACATGAGGCTGGTTTTTGTGTTTACTTGAAAGGGCTA CCATTTGAAGCAGAAAACAAACATGTCATTGATTTTTTTAAAAAGCTGGATATTGTGGAAGATAGTATT TATATAGCTTATGGACCCAATGGGAAAGCAACTGGCGAAGGCTTTGTAGAGTTCAGAAATGAGGCTGAC TATAAGGCTGCTCTGTGTCGTCATAAACAGTACATGGGCAATCGCTTTATTCAAGTTCATCCAATTACT AAGAAAGGTATGCTAGAAAAGATAGATATGATTCGAAAAAGACTGCAGAACTTCAGCTATGACCAGAGG GAAATGATACTAAATCCAGAGGGGGATGTCAACTCTGCCAAAGTCTGTGCCCACATAACAAATATTCCA TTCAGCATTACAAAGATGGATGTTCTTCAGTTCCTAGAAGGAATCCCAGTGGATGAAAATGCTGTACAT GTTCTTGTTGATAACAATGGGCAAGGTCTAGGACAGGCATTGGTTCAGTTTAAAAATGAAGATGATGCA CGTAAGTCTGAACGCTTACACCGTAAAAAACTTAATGGGAGAGAAGCTTTTGTTCATGTAGTTACCCTA GAAGATATGAGAGAGATTGAGAAAAATCCCCCTGCCCAAGGAAAAAAGGGATTAAAGATGCCTGTGCCA GGTAATCCTGCAGTTCCAGGAATGCCCAATGCGGGACTGCCCGGTGTGGGACTGCCCAGTGCAGGACTT CCCGGTGCAGGCCTGCCCAGCACAGGACTGCCTGGTTCAGCAATAACCAGTGCAGGACTGCCTGGTGCG GGAATGCCCAGTGCAGGAATACCTAGTGCAGGAGGTGAAGAGCATGCCTTCCTGACTGTAGGATCAAAG GAAGCCAATAATGGGCCTCCATTTAACTTTCCTGGTAATTTTGGTGGATCAAATGCCTTTGGGCCACCA ATCCCTCCTCCAGGATTAGGAGGCGGGGCCTTTGGTGATGCTAGGCCTGGTATGCCTTCAGTTGGAAAC AGTGGTTTGCCTGGTCTAGGACTGGATGTTCCGGGTTTTGGAGGTGGACCAAACAATTTAAGTGGGCCA TCGGGATTTGGAGGGGGCCCTCAGAATTTTGGAAATGGCCCTGGTAGCTTAGGCGGTCCCCCGGGGTTT GGAAGTGGCCCTCCTGGTCTTGGAAGTGCCCCTGGGCATTTGGGTGGGCCACCAGCTTTTGGGCCTGGC CCCGGCCCCGGCCCCGGCCCTGGCCCAATCCATATTGGTGGTCCCCCTGGCTTTGCATCTAGTTCTGGA AAACCAGGACCGACAGTAATTAAAGTGCAAAACATGCCCTTTACTGTGTCTATTGATGAGATTTTAGAT TTCTTTTATGGCTATCAAGTAATCCCAGGCTCAGTGTGTTTAAAATACAATGAAAAAGGTATGCCCACA GGTGAAGCCATGGTGGCCTTTGAGTCTCGGGATGAAGCCACAGCTGCTGTCATTGACTTAAATGACAGG CCTATAGGTTCAAGAAAAGTAAAACTTGTATTAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC235053 Blue=ORF Red=Cloning site Green=Tag(s) MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGELGEAFIVFATDEDARLGMMRTGGTIKGSKV TLLLSSKTEMQNMIELSRRRFETANLDIPPANASRSGPPPSSGMSSRVNLPTTVSNFNNPSPSVVTATT SVHESNKNIQTFSTASVGTAPPNMGASFGSPTFSSTVPSTASPMNTVPPPPIPPIPAMPSLPPMPSIPP IPVPPPVPTLPPVPPVPPIPPVPSVPPMTPLPPMSGMPPLNPPPVAPLPAGMNGSGAPMNLNNNLNPMF LGPLNPVNPIQMNSQSSVKPLPINPDDLYVSVHGMPFSAMENDVRDFFHGLRVDAVHLLKDHVGRNNGN GLVKFLSPQDTFEALKRNRMLMIQRYVEVSPATERQWVAAGGHITFKQNMGPSGQTHPPPQTLPRSKSP SGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKATGEGFVEFRNEAD YKAALCRHKQYMGNRFIQVHPITKKGMLEKIDMIRKRLQNFSYDQREMILNPEGDVNSAKVCAHITNIP FSITKMDVLQFLEGIPVDENAVHVLVDNNGQGLGQALVQFKNEDDARKSERLHRKKLNGREAFVHVVTL EDMREIEKNPPAQGKKGLKMPVPGNPAVPGMPNAGLPGVGLPSAGLPGAGLPSTGLPGSAITSAGLPGA GMPSAGIPSAGGEEHAFLTVGSKEANNGPPFNFPGNFGGSNAFGPPIPPPGLGGGAFGDARPGMPSVGN SGLPGLGLDVPGFGGGPNNLSGPSGFGGGPQNFGNGPGSLGGPPGFGSGPPGLGSAPGHLGGPPAFGPG PGPGPGPGPIHIGGPPGFASSSGKPGPTVIKVQNMPFTVSIDEILDFFYGYQVIPGSVCLKYNEKGMPT GEAMVAFESRDEATAAVIDLNDRPIGSRKVKLVLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198840
ORF Size	2796 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198840.2
RefSeq Size	6639 bp
RefSeq ORF	2799 bp
Locus ID	10137
UniProt ID	Q9NTZ6
MW	97.4 kDa
Gene Summary	This gene encodes a protein that contains several RNA-binding motifs, potential transmembrane domains, and proline-rich regions. This gene and the gene for copine I overlap at map location 20q11.21. Alternative splicing in the 5' UTR results in four transcript variants. All variants encode the same protein. [provided by RefSeq, Nov 2010]

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