TUBGCP2 (NM_001256617) Human Tagged ORF Clone
CAT#: RC235048
- TrueORF®
TUBGCP2 (Myc-DDK tagged) - Homo sapiens tubulin, gamma complex associated protein 2 (TUBGCP2), transcript variant 1
ORF Plasmid: tGFP
"NM_001256617" in other vectors (2)
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CNY 7,130.00
Cited in 2 publications. |
CNY 300.00
CNY 6,281.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALP4; GCP-2; GCP2; Grip103; h103p; hGCP2; hSpc97; PAMDDFS; SPBC97; SPC97; Spc97p |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235048 representing NM_001256617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAATTTCGGATTCACCATGACGTCAATGAACTGCTTAGCCTGCTGCGTGTCCACGGAGGAGATG GGGCTGAGGTCTACATTGACCTGCTTCAAAAGAACAGGACCCCGTACGTCACTACCACTGTCTCTGCTCA CAGTGCCAAGGTTAAAATTGCAGAGTTTTCTCGTACTCCAGAAGACTTTCTAAAGAAATATGATGAACTG AAATCTAAAAATACAAGGAACCTTGACCCGCTGGTGTACCTGTTGTCAAAGCTCACGGAAGACAAAGAGA CTCTGCAGTACTTACAACAGAATGCAAAAGAAAGAGCTGAGCTTGCAGCCGCTGCTGTGGGCAGCAGTAC CACCAGCATCAACGTCCCTGCCGCGGCCTCCAAGATCTCCATGCAGGAGCTTGAGGAACTGAGGAAGCAG CTTGGCAGCGTGGCCACAGGCTCCACGCTGCAGCAGTCTCTGGAACTTAAAAGAAAGATGCTTCGAGACA AGCAGAACAAAAAAAATTCAGGCCAGCACCTCCCCATCTTCCCAGCATGGGTGTATGAGAGACCTGCCCT GATCGGGGATTTCCTGATTGGTGCTGGCATCAGCACAGACACCGCTTTGCCGATAGTTCTTTTGAGATGG AATCTCGCTCTGTCGCCCAGGCTGAAGTGCAGTGGCGTGATCTCGGCACACTGCAACCTCCACCTCCCAG GCACGTTGCCCCTGGCCTCGCAGGAGTCGGCCGTGGTGGAGGACCTGCTGTACGTGCTGGTGGGCGTGGA CGGGAGGTACGTCAGTGCTCAGCCCCTGGCTGGGAGGCAGAGCCGGACCTTCCTCGTGGACCCCAACCTG GACCTGTCCATCAGGGAGCTGGTGCACAGGATCCTCCCAGTGGCCGCCAGCTACTCCGCTGTGACCAGGT TCATTGAAGAGAAGTCTTCCTTCGAGTACGGGCAGGTGAACCACGCCCTGGCGGCCGCCATGCGCACCCT GGTGAAGGAGCACCTGATTCTGGTGTCACAGCTGGAGCAGCTGCACAGGCAGGGCCTCCTTTCGCTGCAG AAGCTCTGGTTCTACATCCAGCCAGCCATGCGCACCATGGACATCCTGGCCTCCCTCGCCACCTCGGTGG ACAAAGGCGAATGTCTTGGGGGGTCCACGCTGAGCCTGCTCCACGACAGGAGCTTCAGCTACACAGGGGA CAGCCAGGCGCAGGAGCTATGCCTGTACCTAACCAAGGCGGCCAGTGCTCCCTACTTCGAGGTTCTGGAG AAGTGGATCTACAGGGGCATCATCCACGACCCATACAGTGAGTTTATGGTCGAGGAGCACGAGCTGCGGA AGGAGAGGATCCAGGAGGATTACAACGACAAGTACTGGGACCAGCGGTACACCATCGTCCAGCAGCAGAT CCCGTCCTTCCTGCAGAAAATGGCGGACAAGATCCTCAGCACAGGAAAATATCTAAATGTGGTCAGAGAG TGTGGCCATGACGTCACCTGCCCGGTGGCTAAAGAGATCATCTACACGTTAAAAGAGCGGGCGTATGTGG AGCAGATCGAGAAGGCGTTTAACTACGCCAGCAAGGTGCTGCTGGACTTCCTGATGGAGGAGAAGGAGCT GGTGGCTCACCTCAGGTCCATCAAGCGCTACTTCCTCATGGACCAGGGCGACTTCTTCGTGCACTTCATG GACCTCGCGGAGGAGGAGCTCCGGAAGCCGGTGGAGGACATCACGCCCCCTCGCCTGGAAGCGCTCCTGG AGCTGGCGCTGCGCATGAGCACGGCCAACACTGACCCCTTCAAGGACGACCTCAAGATCGACCTGATGCC CCATGACCTCATCACTCAGCTCTTGCGCGTCCTGGCCATCGAGACCAAGCAGGAGAAGGCGATGGCGCAC GCCGACCCCACGGAGCTGGCGCTGAGCGGCCTGGAGGCCTTCTCTTTCGACTACATCGTCAAGTGGCCCC TTTCGCTCATCATCAACAGGAAAGCCCTCACTCGCTACCAGATGCTCTTCAGGCACATGTTCTACTGCAA GCACGTGGAGCGGCAGCTCTGCAGCGTCTGGATCAGCAACAAAACCGCCAAGCAGCACTCGCTGCACTCC GCCCAGTGGTTTGCTGGGGCTTTCACTCTGCGGCAGCGAATGCTCAACTTCGTCCAGAATATTCAATACT ACATGATGTTTGAAGTGATGGAACCGACCTGGCACATCCTGGAGAAAAACCTGAAATCCGCCTCCAACAT TGACGACGTCCTTGGCCACCACACAGGCTTCCTGGACACCTGCCTGAAGGACTGCATGCTCACCAACCCC GAGCTGCTGAAGGTCTTCTCCAAGCTCATGTCTGTGTGCGTCATGTTCACCAACTGCATGCAGAAATTTA CACAGAGCATGAAATTAGATGGCGAGCTGGGCGGGCAGACGCTGGAGCACAGCACCGTCCTGGGGCTGCC CGCAGGGGCCGAGGAGCGGGCCCGGAAGGAGCTCGCCAGGAAGCACCTGGCTGAGCACGCAGACACTGTG CAGCTGGTGTCCGGCTTCGAGGCCACCATCAACAAGTTTGACAAGAACTTCTCAGCCCACCTGCTGGACC TCCTGGCCCGGCTGAGCATCTATAGCACCAGTGACTGTGAGCACGGCATGGCCAGCGTCATCTCCAGGCT TGACTTCAATGGTTTCTACACGGAGCGCCTGGAGCGCCTGTCTGCAGAGAGGAGCCAGAAGGCCACCCCC CAAGTGCCTGTCCTGCGGGGGCCCCCGGCTCCTGCACCCAGGGTCGCAGTCACCGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235048 representing NM_001256617
Red=Cloning site Green=Tags(s) MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTPEDFLKKYDEL KSKNTRNLDPLVYLLSKLTEDKETLQYLQQNAKERAELAAAAVGSSTTSINVPAAASKISMQELEELRKQ LGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTDTALPIVLLRW NLALSPRLKCSGVISAHCNLHLPGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNL DLSIRELVHRILPVAASYSAVTRFIEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQ KLWFYIQPAMRTMDILASLATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLE KWIYRGIIHDPYSEFMVEEHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRE CGHDVTCPVAKEIIYTLKERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFM DLAEEELRKPVEDITPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAH ADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHS AQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNP ELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTV QLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATP QVPVLRGPPAPAPRVAVTAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256617 |
ORF Size | 2790 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256617.1, NP_001243546.1 |
RefSeq Size | 4302 bp |
RefSeq ORF | 2793 bp |
Locus ID | 10844 |
UniProt ID | Q9BSJ2 |
Protein Families | Druggable Genome |
MW | 106.1 kDa |
Gene Summary | Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure
,Consolati, T;Locke, J;Roostalu, J;Chen, ZA;Gannon, J;Asthana, J;Lim, WM;Martino, F;Cvetkovic, MA;Rappsilber, J;Costa, A;Surrey, T;,
Dev. Cell
,PubMed ID 32433913
[TUBGCP2]
|
Microtubule Nucleation by Single Human γTuRC in a Partly Open Asymmetric Conformation
,Consolati, T;Locke, J;Roostalu, J;Asthana, J;Lim, WM;,
bioRxiv
[TUBGCP2]
|
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