eIF3s8 (EIF3C) (NM_001267574) Human Tagged ORF Clone
CAT#: RC235026
- TrueORF®
EIF3C (Myc-DDK tagged) - Homo sapiens eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 4
ORF Plasmid: tGFP
"NM_001267574" in other vectors (2)
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CNY 6,688.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | eIF3-p110; EIF3CL; EIF3S8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235026 representing NM_001267574.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGCGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAGGAGCTCGTC ACCAAACCTGTCGGAGGCAACTATGGCAAACAGCCATTGTTGCTGAGCGAGGATGAAGAAGATACCAAG AGAGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTTATCCGGACCATCCGTAAT GCCATGAAGATTCGTGATGTCACCAAGTGCCTGGAAGAGTTTGAGCTCCTGGGAAAAGCATATGGGAAG GCCAAAAGCATTGTGGACAAAGAAGGTGTCCCCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGAC TATCTTAATGAGCTTTGGGAAGATAAGGAAGGGAAGAAGAAGATGAACAAGAACAATGCCAAGGCTCTG AGCACCTTGCGTCAGAAGATCCGAAAATACAACCGTGATTTCGAGTCCCATATCACAAGCTACAAGCAG AACCCCGAGCAGTCTGCGGATGAAGATGCTGAGAAAAATGAGGAGGATTCAGAAGGCTCTTCAGATGAG GATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTTGAAGAAGAAATCAGAAGCTCCTTCTGGGGAG AGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGAGGACTCAGAAGATTCCGAAGATGATGAAGAC TGGGACACAGGTTCCACATCTTCCGATTCCGACTCAGAGGAGGAAGAAGGGAAACAAACCGCGCTGGCC TCAAGATTTCTTAAAAAGGCACCCACCACAGATGAGGACAAGAAGGCAGCCGAGAAGAAACGGGAGGAC AAAGCTAAGAAGAAGCACGACAGGAAATCCAAGCGCCTGGATGAGGAGGAGGAGGACAATGAAGGCGGG GAGTGGGAAAGGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGAAGCCAAAAATGTTTGCCAAGGGAACT GAGATCACCCATGCTGTTGTTATCAAGAAACTGAATGAGATCCTACAGGCACGAGGCAAGAAGGGAACT GATCGTGCTGCCCAGATTGAGCTGCTGCAACTGCTGGTTCAGATTGCAGCGGAAAACAACCTGGGAGAG GGCGTCATTGTCAAGATCAAGTTCAATATCATCGCCTCTCTCTATGACTACAACCCCAATCTGGCAACC TACATGAAGCCAGAGATGTGGGGGAAGTGCCTGGACTGCATCAATGAGCTGATGGATATCCTGTTTGCA AATCCCAACATTTTTGTTGGAGAGAATATTCTGGAAGAGAGTGAGAACCTGCACAACGCTGACCAGCCA CTGCGTGTCCGTGGCTGCATCCTAACTCTGGTGGAACGAATGGATGAAGAATTTACCAAAATAATGCAA AATACTGACCCTCACTCCCAAGAGTACGTGGAGCACTTGAAGGATGAGGCCCAGGTGTGTGCCATCATC GAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCACTACCGAGGAGGTCTGCCGCATCTACCTGCTGCGC ATCCTGCACACCTACTACAAGTTTGATTACAAGGCCCATCAGCGACAGCTGACCCCGCCTGAGGGCTCC TCAAAGTCTGAGCAAGACCAGGCAGAAAATGAGGGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGC AAGTACATCTACGCCAAGGACCGCACAGACCGGATCCGCACATGTGCCATCCTCTGCCACATCTACCAC CATGCTCTGCACTCGCGCTGGTACCAGGCCCGCGACCTCATGCTCATGAGCCACTTGCAGGACAACATT CAGCATGCAGACCCGCCAGTGCAGATCCTTTACAACCGCACCATGGTGCAGCTGGGCATCTGTGCCTTC CGCCAAGGCCTGACCAAGGACGCACACAACGCCCTGCTGGACATCCAGTCGAGTGGCCGAGCCAAGGAG CTTCTGGGCCAGGGCCTGCTGCTGCGCAGCCTGCAGGAGCGCAACCAGGAGCAGGAGAAGGTGGAGCGG CGCCGTCAGGTCCCCTTCCACCTGCACATCAACCTGGAGCTGCTGGAGTGTGTCTACCTGGTGTCTGCC ATGCTCCTGGAGATCCCCTACATGGCCGCCCATGAGAGCGATGCCCGCCGACGCATGATCAGCAAGCAG TTCCACCACCAGCTGCGCGTGGGCGAGCGACAGCCCCTGCTGGGTCCCCCTGAGTCCATGCGGGAACAT GTGGTCGCTGCCTCCAAGGCCATGAAGATGGGTGACTGGAAGACCTGTCACAGTTTTATCATCAATGAG AAGATGAATGGGAAAGTGTGGGACCTTTTCCCCGAGGCTGACAAAGTCCGCACCATGCTGGTTAGGAAG ATCCAGGAAGAGTCACTGAGGACCTACCTCTTCACCTACAGCAGTGTCTATGACTCCATCAGCATGGAG ACGCTGTCAGACATGTTTGAGCTGGATCTGCCCACTGTGCACTCCATCATCAGCAAAATGATCATTAAT GAGGAGCTGATGGCCTCCCTGGACCAGCCAACACAGACAGTGGTGATGCACCGCACTGAGCCCACTGCC CAGCAGAACCTGGCTCTGCAGCTGGCCGAGAAGCTGGGCAGCCTGGTGGAGAACAACGAACGGGTGTTT GACCACAAGCAGGGCACCTACGGGGGCTACTTCCGAGACCAGAAGGACGGCTACCGCAAAAACGAGGGC TACATGCGCCGCGGTGGCTACCGCCAGCAGCAGTCTCAGACGGCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC235026
Blue=ORF Red=Cloning site Green=Tag(s) MSRFFTTGSDSESESSLSGEELVTKPVGGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRN AMKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKAL STLRQKIRKYNRDFESHITSYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVSAATFLKKKSEAPSGE SRKFLKKMDDEDEDSEDSEDDEDWDTGSTSSDSDSEEEEGKQTALASRFLKKAPTTDEDKKAAEKKRED KAKKKHDRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGT DRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWGKCLDCINELMDILFA NPNIFVGENILEESENLHNADQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAII ERVQRYLEEKGTTEEVCRIYLLRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLC KYIYAKDRTDRIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAF RQGLTKDAHNALLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSA MLLEIPYMAAHESDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINE KMNGKVWDLFPEADKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIIN EELMASLDQPTQTVVMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEG YMRRGGYRQQQSQTAY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001267574 |
ORF Size | 2739 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 3234 bp |
RefSeq ORF | 2742 bp |
Locus ID | 8663 |
UniProt ID | Q99613 |
Protein Families | Druggable Genome |
MW | 105.3 kDa |
Gene Summary | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG235026 | EIF3C (tGFP-tagged) - Homo sapiens eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 4 |
CNY 4,370.00 |
|
SC332857 | EIF3C (untagged) - Homo sapiens eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 4 |
CNY 8,740.00 |