eIF3s8 (EIF3C) (NM_001267574) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	eIF3-p110; EIF3CL; EIF3S8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235026 representing NM_001267574. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGCGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAGGAGCTCGTC ACCAAACCTGTCGGAGGCAACTATGGCAAACAGCCATTGTTGCTGAGCGAGGATGAAGAAGATACCAAG AGAGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTTATCCGGACCATCCGTAAT GCCATGAAGATTCGTGATGTCACCAAGTGCCTGGAAGAGTTTGAGCTCCTGGGAAAAGCATATGGGAAG GCCAAAAGCATTGTGGACAAAGAAGGTGTCCCCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGAC TATCTTAATGAGCTTTGGGAAGATAAGGAAGGGAAGAAGAAGATGAACAAGAACAATGCCAAGGCTCTG AGCACCTTGCGTCAGAAGATCCGAAAATACAACCGTGATTTCGAGTCCCATATCACAAGCTACAAGCAG AACCCCGAGCAGTCTGCGGATGAAGATGCTGAGAAAAATGAGGAGGATTCAGAAGGCTCTTCAGATGAG GATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTTGAAGAAGAAATCAGAAGCTCCTTCTGGGGAG AGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGAGGACTCAGAAGATTCCGAAGATGATGAAGAC TGGGACACAGGTTCCACATCTTCCGATTCCGACTCAGAGGAGGAAGAAGGGAAACAAACCGCGCTGGCC TCAAGATTTCTTAAAAAGGCACCCACCACAGATGAGGACAAGAAGGCAGCCGAGAAGAAACGGGAGGAC AAAGCTAAGAAGAAGCACGACAGGAAATCCAAGCGCCTGGATGAGGAGGAGGAGGACAATGAAGGCGGG GAGTGGGAAAGGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGAAGCCAAAAATGTTTGCCAAGGGAACT GAGATCACCCATGCTGTTGTTATCAAGAAACTGAATGAGATCCTACAGGCACGAGGCAAGAAGGGAACT GATCGTGCTGCCCAGATTGAGCTGCTGCAACTGCTGGTTCAGATTGCAGCGGAAAACAACCTGGGAGAG GGCGTCATTGTCAAGATCAAGTTCAATATCATCGCCTCTCTCTATGACTACAACCCCAATCTGGCAACC TACATGAAGCCAGAGATGTGGGGGAAGTGCCTGGACTGCATCAATGAGCTGATGGATATCCTGTTTGCA AATCCCAACATTTTTGTTGGAGAGAATATTCTGGAAGAGAGTGAGAACCTGCACAACGCTGACCAGCCA CTGCGTGTCCGTGGCTGCATCCTAACTCTGGTGGAACGAATGGATGAAGAATTTACCAAAATAATGCAA AATACTGACCCTCACTCCCAAGAGTACGTGGAGCACTTGAAGGATGAGGCCCAGGTGTGTGCCATCATC GAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCACTACCGAGGAGGTCTGCCGCATCTACCTGCTGCGC ATCCTGCACACCTACTACAAGTTTGATTACAAGGCCCATCAGCGACAGCTGACCCCGCCTGAGGGCTCC TCAAAGTCTGAGCAAGACCAGGCAGAAAATGAGGGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGC AAGTACATCTACGCCAAGGACCGCACAGACCGGATCCGCACATGTGCCATCCTCTGCCACATCTACCAC CATGCTCTGCACTCGCGCTGGTACCAGGCCCGCGACCTCATGCTCATGAGCCACTTGCAGGACAACATT CAGCATGCAGACCCGCCAGTGCAGATCCTTTACAACCGCACCATGGTGCAGCTGGGCATCTGTGCCTTC CGCCAAGGCCTGACCAAGGACGCACACAACGCCCTGCTGGACATCCAGTCGAGTGGCCGAGCCAAGGAG CTTCTGGGCCAGGGCCTGCTGCTGCGCAGCCTGCAGGAGCGCAACCAGGAGCAGGAGAAGGTGGAGCGG CGCCGTCAGGTCCCCTTCCACCTGCACATCAACCTGGAGCTGCTGGAGTGTGTCTACCTGGTGTCTGCC ATGCTCCTGGAGATCCCCTACATGGCCGCCCATGAGAGCGATGCCCGCCGACGCATGATCAGCAAGCAG TTCCACCACCAGCTGCGCGTGGGCGAGCGACAGCCCCTGCTGGGTCCCCCTGAGTCCATGCGGGAACAT GTGGTCGCTGCCTCCAAGGCCATGAAGATGGGTGACTGGAAGACCTGTCACAGTTTTATCATCAATGAG AAGATGAATGGGAAAGTGTGGGACCTTTTCCCCGAGGCTGACAAAGTCCGCACCATGCTGGTTAGGAAG ATCCAGGAAGAGTCACTGAGGACCTACCTCTTCACCTACAGCAGTGTCTATGACTCCATCAGCATGGAG ACGCTGTCAGACATGTTTGAGCTGGATCTGCCCACTGTGCACTCCATCATCAGCAAAATGATCATTAAT GAGGAGCTGATGGCCTCCCTGGACCAGCCAACACAGACAGTGGTGATGCACCGCACTGAGCCCACTGCC CAGCAGAACCTGGCTCTGCAGCTGGCCGAGAAGCTGGGCAGCCTGGTGGAGAACAACGAACGGGTGTTT GACCACAAGCAGGGCACCTACGGGGGCTACTTCCGAGACCAGAAGGACGGCTACCGCAAAAACGAGGGC TACATGCGCCGCGGTGGCTACCGCCAGCAGCAGTCTCAGACGGCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC235026 Blue=ORF Red=Cloning site Green=Tag(s) MSRFFTTGSDSESESSLSGEELVTKPVGGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRN AMKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKAL STLRQKIRKYNRDFESHITSYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVSAATFLKKKSEAPSGE SRKFLKKMDDEDEDSEDSEDDEDWDTGSTSSDSDSEEEEGKQTALASRFLKKAPTTDEDKKAAEKKRED KAKKKHDRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGT DRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWGKCLDCINELMDILFA NPNIFVGENILEESENLHNADQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAII ERVQRYLEEKGTTEEVCRIYLLRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLC KYIYAKDRTDRIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAF RQGLTKDAHNALLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSA MLLEIPYMAAHESDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINE KMNGKVWDLFPEADKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIIN EELMASLDQPTQTVVMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEG YMRRGGYRQQQSQTAY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001267574
ORF Size	2739 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	3234 bp
RefSeq ORF	2742 bp
Locus ID	8663
UniProt ID	Q99613
Protein Families	Druggable Genome
MW	105.3 kDa
Gene Summary	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function]

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