LEPRE1 (P3H1) (NM_001243246) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GROS1; LEPRE1; OI8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234906 representing NM_001243246 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCTGCCTCCCAAGCCGAGG TCGAGTCCGAGGCAGGATGGGGCATGGTGACGCCTGATCTGCTCTTCGCCGAGGGGACCGCAGCCTACGC GCGCGGGGACTGGCCCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTCCGCGCC CTTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGGGAGCTGGACCCCGACTGGTCCCCCA GCCCGGCCCAGGCCTCGGGCGCCGCCGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGTCGCGC TGCCTGCCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGATGGAGCTGGAGTTC CGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCCTACTTCAAGATCAACAAGTTGGAGAAAGCTGTTG CTGCAGCACACACCTTCTTCGTGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGACTATTACCA AACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACTCAACCCCATATGCAAGAATTTCGA CTGGGAGTGCGACTCTACTCAGAGGAACAGCCACAGGAAGCTGTGCCCCACCTAGAGGCGGCGCTGCAAG AATACTTTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGATGGCTACAACTA CCTTGAGTACAACGCTGACCTCTTCCAGGCCATCACAGATCATTACATCCAGGTCCTCAACTGTAAGCAG AACTGTGTCACGGAGCTTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTCCTCCCATCGCATT ATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGGCTGTTGAATGTGCCAAGACCTA TCTTCTCTTCTTCCCCAATGACGAGGTGATGAACCAAAATTTGGCCTATTATGCAGCTATGCTTGGAGAA GAACACACCAGATCCATCGGCCCCCGTGAGAGTGCCAAGGAGTACCGACAGCGAAGCCTACTGGAAAAAG AACTGCTTTTCTTCGCTTATGATGTTTTTGGAATTCCCTTTGTGGATCCGGATTCATGGACTCCAGAAGA AGTGATTCCCAAGAGATTGCAAGAGAAACAGAAGTCAGAACGGGAAACAGCCGTACGCATCTCCCAGGAG ATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCAAGGAGTCACTGGATGTGAGCA GACTGACCCGGGAAGGTGGCCCCCTGCTGTATGAAGGCATCAGTCTCACCATGAACTCCAAACTCCTGAA TGGTTCCCAGCGGGTGGTGATGGACGGCGTAATCTCTGACCACGAGTGTCAGGAGCTGCAGAGACTGACC AATGTGGCAGCAACCTCAGGAGATGGCTACCGGGGTCAGACCTCCCCACATACTCCCAATGAAAAGTTCT ATGGTGTCACTGTCTTCAAAGCCCTCAAGCTGGGGCAAGAAGGCAAAGTTCCTCTGCAGAGTGCCCACCT GTACTACAACGTGACGGAGAAGGTGCGGCGCATCATGGAGTCCTACTTCCGCCTGGATACGCCCCTCTAC TTTTCCTACTCTCATCTGGTGTGCCGCACTGCCATCGAAGAGGTCCAGGCAGAGAGGAAGGATGATAGTC ATCCAGTCCACGTGGACAACTGCATCCTGAATGCCGAGACCCTCGTGTGTGTCAAAGAGCCCCCAGCCTA CACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGGGACTTCGATGGCGGAAACTTTTATTTCACT GAACTGGATGCCAAGACCGTGACGGCAGAGGTGCAGCCTCAGTGTGGAAGAGCCGTGGGATTCTCTTCAG GCACTGAAAACCCACATGGAGTGAAGGCTGTCACCAGGGGGCAGCGCTGTGCCATCGCCCTGTGGTTCAC CCTGGACCCTCGACACAGCGAGCGGGTGAGAGCAGCTCGAGCGGGTGAGAGCAGCTGGTGCTGTGGTGAC CCGTTCCCAGAGCGCCCTTGGTTTGCCTTTCTCTTCCCCAAATCCCATTGCCAGTGGCTGAGACACGAAA GGAGCACTTGGGACACCAGCTCCAACGCCCTGTCATTATGGTCACATTGCCTTGTCCTCCCTGGGCCTGC TGTGAACGGGATCCAGGTGGGGAAAGAGGTCAAGACAGGGAGCGATGCTGAGTTCTTGGTTCCCTCCTTG GGCCCCACTTCAGCTGTCCTTTTCCAGAGAGTAGGACCTGCTGGGAAGGAGATGAGCCTGGGGCCATTAA GGAACCTTCCTTGTCCCCTGGGAAGTAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234906 representing NM_001243246 Red=Cloning site Green=Tags(s) MAVRALKLLTTLLAVVAAASQAEVESEAGWGMVTPDLLFAEGTAAYARGDWPGVVLSMERALRSRAALRA LRLRCRTQCAADFPWELDPDWSPSPAQASGAAALRDLSFFGGLLRRAACLRRCLGPPAAHSLSEEMELEF RKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMQQNLDYYQTMSGVKEADFKDLETQPHMQEFR LGVRLYSEEQPQEAVPHLEAALQEYFVAYEECRALCEGPYDYDGYNYLEYNADLFQAITDHYIQVLNCKQ NCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAVECAKTYLLFFPNDEVMNQNLAYYAAMLGE EHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQE IGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKLLNGSQRVVMDGVISDHECQELQRLT NVAATSGDGYRGQTSPHTPNEKFYGVTVFKALKLGQEGKVPLQSAHLYYNVTEKVRRIMESYFRLDTPLY FSYSHLVCRTAIEEVQAERKDDSHPVHVDNCILNAETLVCVKEPPAYTFRDYSAILYLNGDFDGGNFYFT ELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERVRAARAGESSWCCGD PFPERPWFAFLFPKSHCQWLRHERSTWDTSSNALSLWSHCLVLPGPAVNGIQVGKEVKTGSDAEFLVPSL GPTSAVLFQRVGPAGKEMSLGPLRNLPCPLGSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243246
ORF Size	2412 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243246.2
RefSeq Size	3115 bp
RefSeq ORF	2415 bp
Locus ID	64175
UniProt ID	Q32P28
Protein Families	Secreted Protein
MW	91.1 kDa
Gene Summary	This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Three alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined. [provided by RefSeq, Aug 2011]

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