CPT1C (NM_001199753) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CATL1; CPT1-B; CPT1P; CPTI-B; CPTIC; SPG73
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234902 representing NM_001199753. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGAAGCGCACCAGGCCGTGGGCTTCCGACCCTCGCTGACCTCGGACGGGGCTGAAGTGGAACTC AGTGCCCCTGTGCTGCAGGAGATCTACCTCTCTGGCCTGCGCTCCTGGAAAAGGCATCTCTCACGTTTC TGGAATGACTTTCTCACCGGTGTGTTTCCTGCCAGCCCCCTCAGTTGGCTTTTCCTCTTCAGTGCCATC CAGCTTGCCTGGTTCCTCCAGCTGGATCCTTCCTTAGGACTGATGGAGAAGATCAAAGAGTTGCTGCCT GACTGGGGTGGACAACACCACGGGCTCCGGGGGGTCCTGGCAGCCGCGCTGTTTGCCTCGTGTTTGTGG GGAGCCCTGATCTTCACACTGCACGTGGCCCTGAGGCTGCTTCTGTCCTACCACGGCTGGCTTCTTGAG CCCCACGGAGCCATGTCCTCCCCCACCAAGACCTGGCTGGCCCTGGTCCGCATCTTCTCTGGCCGCCAC CCGATGCTGTTCAGTTACCAGCGCTCCCTGCCACGCCAGCCCGTGCCCTCTGTGCAGGACACCGTGCGC AAGTACCTGGAGTCGGTCCGGCCCATCCTCTCCGACGAGGACTTCGACTGGACCGCGGTCCTGGCGCAG GAATTCCTGAGGCTGCAGGCGTCGCTGCTGCAGTGGTACCTGCGGCTCAAGTCCTGGTGGGCGTCCAAT TATGTCAGTGACTGGTGGGAGGAATTTGTGTACCTGCGCTCCCGAAATCCGCTGATGGTGAACAGCAAC TATTACATGATGGACTTCCTGTATGTCACACCCACGCCTCTGCAGGCAGCTCGCGCTGGGAATGCCGTC CATGCCCTCCTCCTGTACCGCCACCGCCTGAACCGCCAGGAGATACCCCCGACTTTGCTGATGGGAATG CGCCCCTTATGCTCTGCCCAGTACGAGAAGATCTTCAACACCACGCGGATTCCAGGGGTCCAAAAAGAC TACATCCGCCACCTCCATGACAGCCAACACGTGGCTGTCTTCCACCGGGGCCGATTCTTCCGCATGGGG ACCCACTCCCGAAACAGCCTGCTTTCCCCGAGAGCCCTGGAGCAGCAGTTTCAGAGAATCCTGGATGAT CCCTCACCGGCCTGCCCCCACGAGGAACATCTGGCAGCTCTGACAGCTGCTCCCAGGGGCACGTGGGCC CAGGTGCGGACATCCCTGAAGACCCAGGCAGCGGAGGCCCTGGAGGCGGTGGAAGGGGCCGCTTTCTTT GTGTCACTGGATGCTGAGCCCGCGGGGCTCACCAGGGAGGACCCGGCAGCGTCGTTGGATGCCTACGCC CATGCTCTGCTGGCCGGCCGGGGCCATGATCGCTGGTTTGACAAATCCTTCACCCTAATCGTCTTCTCT AACGGGAAGCTGGGCCTCAGCGTGGAGCACTCCTGGGCCGACTGCCCCATCTCAGGACACATGTGGGAG TTCACTCTGGCTACAGAATGCTTTCAGCTGGGCTACTCAACAGACGGCCACTGCAAGGGGCACCCGGAC CCCACACTACCCCAGCCCCAGCGGCTGCAATGGGACCTTCCAGACCAGATCCACTCCTCCATCTCTCTA GCCCTGAGGGGAGCCAAGATCTTGTCTGAAAATGTCGACTGCCATGTCGTTCCATTCTCCCTATTTGGC AAGAGCTTCATCCGACGCTGCCACCTCTCTTCAGACAGCTTCATCCAGATCGCCTTGCAACTGGCCCAC TTCCGGGACAGGGGTCAATTCTGCCTGACTTATGAGTCGGCCATGACTCGCTTATTCCTGGAAGGCCGG ACGGAGACGGTGCGGTCTTGCACGAGGGAGGCCTGCAACTTTGTCAGGGCCATGGAGGACAAAGAGAAG ACGGACCCACAGTGCCTCGCCCTGTTCCGCGTGGCAGTGGACAAGCACCAGGCTCTGCTGAAGGCAGCC ATGAGCGGGCAGGGAGTTGACCGCCACCTGTTTGCGCTGTACATCGTGTCCCGATTCCTCCACCTGCAG TCGCCCTTCCTGACCCAGGTCCATTCGGAGCAGTGGCAGCTGTCCACCAGCCAGATCCCTGTTCAGCAA ATGCATCTGTTTGACGTCCACAATTACCCGGACTATGTTTCCTCAGGCGGTGGATTCGGGCCTGCTGAT GACCATGGTTATGGTGTTTCTTATATCTTCATGGGGGATGGCATGATCACCTTCCACATCTCCAGCAAA AAATCAAGCACAAAAACGGATTCCCACAGGCTGGGGCAGCACATTGAGGACGCACTGCTGGATGTGGCC TCCCTGTTCCAGGCGGGACAGCATTTTAAGCGCCGGTTCAGAGGGTCAGGGAAGGAGAACTCCAGGCAC AGGTGTGGATTTCTCTCCCGCCAGACTGGGGCCTCCAAGGCCTCAATGACATCCACCGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC234902 Blue=ORF Red=Cloning site Green=Tag(s) MAEAHQAVGFRPSLTSDGAEVELSAPVLQEIYLSGLRSWKRHLSRFWNDFLTGVFPASPLSWLFLFSAI QLAWFLQLDPSLGLMEKIKELLPDWGGQHHGLRGVLAAALFASCLWGALIFTLHVALRLLLSYHGWLLE PHGAMSSPTKTWLALVRIFSGRHPMLFSYQRSLPRQPVPSVQDTVRKYLESVRPILSDEDFDWTAVLAQ EFLRLQASLLQWYLRLKSWWASNYVSDWWEEFVYLRSRNPLMVNSNYYMMDFLYVTPTPLQAARAGNAV HALLLYRHRLNRQEIPPTLLMGMRPLCSAQYEKIFNTTRIPGVQKDYIRHLHDSQHVAVFHRGRFFRMG THSRNSLLSPRALEQQFQRILDDPSPACPHEEHLAALTAAPRGTWAQVRTSLKTQAAEALEAVEGAAFF VSLDAEPAGLTREDPAASLDAYAHALLAGRGHDRWFDKSFTLIVFSNGKLGLSVEHSWADCPISGHMWE FTLATECFQLGYSTDGHCKGHPDPTLPQPQRLQWDLPDQIHSSISLALRGAKILSENVDCHVVPFSLFG KSFIRRCHLSSDSFIQIALQLAHFRDRGQFCLTYESAMTRLFLEGRTETVRSCTREACNFVRAMEDKEK TDPQCLALFRVAVDKHQALLKAAMSGQGVDRHLFALYIVSRFLHLQSPFLTQVHSEQWQLSTSQIPVQQ MHLFDVHNYPDYVSSGGGFGPADDHGYGVSYIFMGDGMITFHISSKKSSTKTDSHRLGQHIEDALLDVA SLFQAGQHFKRRFRGSGKENSRHRCGFLSRQTGASKASMTSTDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199753
ORF Size	2409 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199753.2
RefSeq Size	2883 bp
RefSeq ORF	2412 bp
Locus ID	126129
UniProt ID	Q8TCG5
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
MW	91 kDa
Gene Summary	This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

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