TUBGCP2 (NM_001256618) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ALP4; GCP-2; GCP2; Grip103; h103p; hGCP2; hSpc97; PAMDDFS; SPBC97; SPC97; Spc97p
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234866 representing NM_001256618 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAGCTTGAGGAACTGAGGAAGCAGCTTGGCAGCGTGGCCACAGGCTCCACGCTGCAGCAGTCTC TGGAACTTAAAAGAAAGATGCTTCGAGACAAGCAGAACAAAAAAAATTCAGGCCAGCACCTCCCCATCTT CCCAGCATGGGTGTATGAGAGACCTGCCCTGATCGGGGATTTCCTGATTGGTGCTGGCATCAGCACAGAC ACCGCTTTGCCGATAGGCACGTTGCCCCTGGCCTCGCAGGAGTCGGCCGTGGTGGAGGACCTGCTGTACG TGCTGGTGGGCGTGGACGGGAGGTACGTCAGTGCTCAGCCCCTGGCTGGGAGGCAGAGCCGGACCTTCCT CGTGGACCCCAACCTGGACCTGTCCATCAGGGAGCTGGTGCACAGGATCCTCCCAGTGGCCGCCAGCTAC TCCGCTGTGACCAGGTTCATTGAAGAGAAGTCTTCCTTCGAGTACGGGCAGGTGAACCACGCCCTGGCGG CCGCCATGCGCACCCTGGTGAAGGAGCACCTGATTCTGGTGTCACAGCTGGAGCAGCTGCACAGGCAGGG CCTCCTTTCGCTGCAGAAGCTCTGGTTCTACATCCAGCCAGCCATGCGCACCATGGACATCCTGGCCTCC CTCGCCACCTCGGTGGACAAAGGCGAATGTCTTGGGGGGTCCACGCTGAGCCTGCTCCACGACAGGAGCT TCAGCTACACAGGGGACAGCCAGGCGCAGGAGCTATGCCTGTACCTAACCAAGGCGGCCAGTGCTCCCTA CTTCGAGGTTCTGGAGAAGTGGATCTACAGGGGCATCATCCACGACCCATACAGTGAGTTTATGGTCGAG GAGCACGAGCTGCGGAAGGAGAGGATCCAGGAGGATTACAACGACAAGTACTGGGACCAGCGGTACACCA TCGTCCAGCAGCAGATCCCGTCCTTCCTGCAGAAAATGGCGGACAAGATCCTCAGCACAGGAAAATATCT AAATGTGGTCAGAGAGTGTGGCCATGACGTCACCTGCCCGGTGGCTAAAGAGATCATCTACACGTTAAAA GAGCGGGCGTATGTGGAGCAGATCGAGAAGGCGTTTAACTACGCCAGCAAGGTGCTGCTGGACTTCCTGA TGGAGGAGAAGGAGCTGGTGGCTCACCTCAGGTCCATCAAGCGCTACTTCCTCATGGACCAGGGCGACTT CTTCGTGCACTTCATGGACCTCGCGGAGGAGGAGCTCCGGAAGCCGGTGGAGGACATCACGCCCCCTCGC CTGGAAGCGCTCCTGGAGCTGGCGCTGCGCATGAGCACGGCCAACACTGACCCCTTCAAGGACGACCTCA AGATCGACCTGATGCCCCATGACCTCATCACTCAGCTCTTGCGCGTCCTGGCCATCGAGACCAAGCAGGA GAAGGCGATGGCGCACGCCGACCCCACGGAGCTGGCGCTGAGCGGCCTGGAGGCCTTCTCTTTCGACTAC ATCGTCAAGTGGCCCCTTTCGCTCATCATCAACAGGAAAGCCCTCACTCGCTACCAGATGCTCTTCAGGC ACATGTTCTACTGCAAGCACGTGGAGCGGCAGCTCTGCAGCGTCTGGATCAGCAACAAAACCGCCAAGCA GCACTCGCTGCACTCCGCCCAGTGGTTTGCTGGGGCTTTCACTCTGCGGCAGCGAATGCTCAACTTCGTC CAGAATATTCAATACTACATGATGTTTGAAGTGATGGAACCGACCTGGCACATCCTGGAGAAAAACCTGA AATCCGCCTCCAACATTGACGACGTCCTTGGCCACCACACAGGCTTCCTGGACACCTGCCTGAAGGACTG CATGCTCACCAACCCCGAGCTGCTGAAGGTCTTCTCCAAGCTCATGTCTGTGTGCGTCATGTTCACCAAC TGCATGCAGAAATTTACACAGAGCATGAAATTAGATGGCGAGCTGGGCGGGCAGACGCTGGAGCACAGCA CCGTCCTGGGGCTGCCCGCAGGGGCCGAGGAGCGGGCCCGGAAGGAGCTCGCCAGGAAGCACCTGGCTGA GCACGCAGACACTGTGCAGCTGGTGTCCGGCTTCGAGGCCACCATCAACAAGTTTGACAAGAACTTCTCA GCCCACCTGCTGGACCTCCTGGCCCGGCTGAGCATCTATAGCACCAGTGACTGTGAGCACGGCATGGCCA GCGTCATCTCCAGGCTTGACTTCAATGGTTTCTACACGGAGCGCCTGGAGCGCCTGTCTGCAGAGAGGAG CCAGAAGGCCACCCCCCAAGTGCCTGTCCTGCGGGGGCCCCCGGCTCCTGCACCCAGGGTCGCAGTCACC GCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234866 representing NM_001256618 Red=Cloning site Green=Tags(s) MQELEELRKQLGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTD TALPIGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNLDLSIRELVHRILPVAASY SAVTRFIEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRTMDILAS LATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLEKWIYRGIIHDPYSEFMVE EHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRECGHDVTCPVAKEIIYTLK ERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLAEEELRKPVEDITPPR LEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDY IVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFV QNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTN CMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFS AHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVT AQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256618
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256618.1, NP_001243547.1
RefSeq Size	4029 bp
RefSeq ORF	2319 bp
Locus ID	10844
UniProt ID	Q9BSJ2
Protein Families	Druggable Genome
MW	88.5 kDa
Gene Summary	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]

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