CRAT (NM_001257363) Human Tagged ORF Clone
CAT#: RC234551
- TrueORF®
CRAT (Myc-DDK tagged) - Homo sapiens carnitine O-acetyltransferase (CRAT), transcript variant 2
ORF Plasmid: tGFP
"NM_001257363" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 4,504.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CAT; CAT1; NBIA8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234551 representing NM_001257363
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCACGGCTGCCCGTGCCCCCTCTCCAGC AGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCA GCTGGTGGATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCC AGGAAGACGGAGAACTGGCTGTCTGAGTGGTGGCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGG TCATCTACTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCCGATT TGCTGCCAAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAG TACCTGGGGGGGAAGCCACTGTGCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCC CCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCACATCACCGTGGTACACAA CTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTG CAGCTGGAGAAGATCTGGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACC ACCGCAACTCCTGGGCCAAGGCATACAACACCCTCATCAAAGACAAGGTGAACCGGGATTCCGTGCGCTC CATCCAGAAGAGCATCTTCACCGTGTGCCTAGATGCAACCATGCCCAGGGTCTCAGAAGACGTGTACCGC AGCCACGTGGCAGGCCAGATGCTGCATGGGGGCGGCAGCAGGCTCAACAGCGGCAACCGCTGGTTCGACA AGACGCTGCAGTTCATCGTGGCAGAAGATGGCTCCTGTGGGCTTGTGTACGAGCATGCTGCAGCGGAGGG GCCCCCTATTGTCACCCTTCTGGACTATGTCATCGAGTACACGAAGAAACCCGAGCTTGTGCGGTCTCCC ATGGTGCCCCTGCCCATGCCCAAGAAGCTGCGGTTCAACATCACCCCCGAGATCAAGAGCGACATCGAGA AGGCCAAGCAGAACCTCAGCATCATGATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTTGG AAAAGACTTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATGGCTTTGCAGCTGGCCTAC TACAGGATCTACGGACAGGCATGTGCCACCTATGAAAGTGCCTCCCTGCGCATGTTTCACCTGGGCCGCA CCGACACCATCCGCTCGGCTTCCATGGACTCACTCACCTTTGTCAAGGCCATGGATGACTCCAGCGTCAC GGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGCCCACCGAGGCTACACCGACCGGGCCATC CGCGGGGAGGCCTTTGATCGACACCTGCTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGC CCGACATCTTCATGGACACCTCCTACGCCATCGCCATGCACTTCCACCTCTCCACCAGCCAGGTCCCTGC CAAGACAGACTGTGTCATGTTCTTCGGGCCCGTGGTCCCCGACGGCTACGGTGTCTGCTATAACCCCATG GAGGCCCACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCCGCCCGCCTGGCGC ATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCAGAGCCACCCCCGGGCCAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234551 representing NM_001257363
Red=Cloning site Green=Tags(s) MKASSRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQASGGVGERLQKGLERRA RKTENWLSEWWLKTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQLRFAAKLIEGVLDFKVMIDNETLPVE YLGGKPLCMNQYYQILSSCRVPGPKQDTVSNFSKTKKPPTHITVVHNYQFFELDVYHSDGTPLTADQIFV QLEKIWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIKDKVNRDSVRSIQKSIFTVCLDATMPRVSEDVYR SHVAGQMLHGGGSRLNSGNRWFDKTLQFIVAEDGSCGLVYEHAAAEGPPIVTLLDYVIEYTKKPELVRSP MVPLPMPKKLRFNITPEIKSDIEKAKQNLSIMIQDLDITVMVFHHFGKDFPKSEKLSPDAFIQMALQLAY YRIYGQACATYESASLRMFHLGRTDTIRSASMDSLTFVKAMDDSSVTEHQKVELLRKAVQAHRGYTDRAI RGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFHLSTSQVPAKTDCVMFFGPVVPDGYGVCYNPM EAHINFSLSAYNSCAETNAARLAHYLEKALLDMRALLQSHPRAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001257363 |
ORF Size | 1815 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001257363.2 |
RefSeq Size | 2865 bp |
RefSeq ORF | 1818 bp |
Locus ID | 1384 |
Protein Families | Druggable Genome |
MW | 68.6 kDa |
Gene Summary | This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
SDS |