C19orf51 (DNAAF3) (NM_001256715) Human Tagged ORF Clone
CAT#: RC234393
- TrueORF®
DNAAF3 (Myc-DDK tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 3
ORF Plasmid: tGFP
"NM_001256715" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C19orf51; CILD2; DAB1; PCD; PF22 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234393 representing NM_001256715
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACACCTGCCGGCTCCGGCAGCGGCTTCGGCTCCGTGTCCTGGTGGGGCCTGTCCCCGGCGCTGG ACCTGCAGGCTGAAAGTCCTCCTGTGGACCCAGACTCCCAGGCCGATACAGTGCACAGCAACCCCGAGCT AGATGTGCTGCTTCTGGGCTCTGTGGATGGACGGCACCTGCTGCGGACCCTGTCCCGAGCGAAGTTCTGG CCTCGCAGGAGGTTCAACTTCTTTGTGCTGGAGAATAATCTGGAAGCTGTGGCCCGACACATGCTGATCT TCAGCCTAGCCCTGGAGGAACCGGAGAAGATGGGGCTGCAAGAGCGAAGCGAGACCTTCCTGGAAGTGTG GGGGAACGCGCTGCTGCGCCCGCCAGTGGCCGCCTTCGTGCGTGCCCAGGCCGACCTGCTGGCGCACCTG GTCCCCGAGCCCGACCGCCTGGAGGAACAGCTGCCCTGGCTCAGCCTCCGCGCCCTCAAGTTCCGCGAGC GGGATGCCCTGGAGGCCGTATTCCGCTTCTGGGCTGGCGGCGAGAAAGGGCCCCAGGCGTTCCCCATGAG CCGCCTCTGGGACTCGCGCCTGCGCCACTACCTGGGCTCCCGCTACGACGCCCGGCGCGGTGTCAGCGAC TGGGACCTGCGCATGAAGCTGCATGACCGCGGGGCTCAAGTCATTCACCCCCAGGAGTTCCGACGCTGGC GGGACACAGGCGTCGCCTTTGAACTCAGGGACTCCAGCGCCTATCATGTGCCCAACCGGACCCTGGCGTC CGGTCGCCTCCTGAGCTACCGTGGGGAGCGCGTGGCAGCGCGCGGGTACTGGGGGGACATCGCCACGGGG CCCTTCGTGGCCTTCGGCATCGAAGCGGACGACGAGAGCCTCCTGCGGACGAGCAACGGCCAGCCAGTCA AGACGGCCGGGGAGATCACTCAACACAACGTGACGGAGCTGCTCCGCGACGTGGCCGCCTGGGGGCGCGC GAGAGCCACCGGGGGGGACCTGGAGGAGCAGCAGCACGCGGAGGGAAGCCCGGAGCCAGGGACTCCAGCA GCCCCGACCCCGGAATCTTTCACCGTCCACTTCCTGCCGCTCAATTCTGCTCAGACTCTCCACCACAAGA GCTGCTACAACGGCCGATTCCAGCTCCTCTATGTGGCCTGTGGTATGGTCCATCTTCTCATCCCTGAGCT TGGGGCCTGTGTGGCACCCGGAGGGAACTTGATTGTGGAATTAGCCCGGTACCTGGTGGACGTGCGGCAG GAGCAGCTGCAGGGATTCAACACCCGGGTCAGGGAGCTAGCTCAGGCAGCTGGATTTGCTCCACAGACCG GGGCCAGGCCTTCAGAGACCTTCGCACGTTTCTGCAAGTCCCAGGAATCAGCTCTGGGCAACACTGTCCC AGCTGTGGAACCCGGAACTCCGCCCCTTGACATCCTGGCCCAGCCTCTTGAAGCCAGCAACCCAGCCCTT GAGGGCCTGACCCAGCCTCTGCAGGGTGGGACCCCACACTGTGAGCCCTGCCAGCTGCCCTCTGAGTCTC CAGGTTCACTCTCAGAGGTTCTGGCTCAGCCTCAGGGGGCCTTGGCTCCGCCCAACTGTGAGTCAGACTC CAAAACTGGAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234393 representing NM_001256715
Red=Cloning site Green=Tags(s) MTTPAGSGSGFGSVSWWGLSPALDLQAESPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFW PRRRFNFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHL VPEPDRLEEQLPWLSLRALKFRERDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSD WDLRMKLHDRGAQVIHPQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLLSYRGERVAARGYWGDIATG PFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLRDVAAWGRARATGGDLEEQQHAEGSPEPGTPA APTPESFTVHFLPLNSAQTLHHKSCYNGRFQLLYVACGMVHLLIPELGACVAPGGNLIVELARYLVDVRQ EQLQGFNTRVRELAQAAGFAPQTGARPSETFARFCKSQESALGNTVPAVEPGTPPLDILAQPLEASNPAL EGLTQPLQGGTPHCEPCQLPSESPGSLSEVLAQPQGALAPPNCESDSKTGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256715 |
ORF Size | 1623 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256715.2 |
RefSeq Size | 2231 bp |
RefSeq ORF | 1626 bp |
Locus ID | 352909 |
UniProt ID | Q8N9W5 |
Protein Families | Druggable Genome |
MW | 59.9 kDa |
Gene Summary | The protein encoded by this gene is required for the assembly of axonemal inner and outer dynein arms and plays a role in assembling dynein complexes for transport into cilia. Defects in this gene are a cause of primary ciliary dyskinesia type 2 (CILD2). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
Documents
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