ZNF513 (NM_001201459) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HMFT0656; RP58; Zfp513
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234222 representing NM_001201459 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCGAGAGAGACTCGGAAGGAGACTCTCTGGGGGCCAGGCCTGGGCTTCCCTATGGGCTGAGCG ACGATGAGTCTGGGGGCGGCCGGGCACTAAGTGCGGAGAGTGAAGTTGAGGAGCCAGCCAGGGGTCCAGG GGAGGCCAGGGGTGAGAGGCCAGGCCCAGCCTGCCAGCTGTGTGGGGGGCCGACAGGTGAGGGGCCGTGT TGTGGGGCAGGAGGGCCGGGTGGGGGGCCCCTGCTGCCCCCACGGCTACTGTACTCATGCCGCCTCTGCA CCTTCGTGTCCCACTACTCGAGCCACCTGAAGCGGCACATGCAGACACACAGCGGAGAGAAGCCGTTCCG CTGTGGCCGCTGCCCCTACGCCTCAGCCCAGCTCGTCAACCTGACACGACATACCCGCACCCACACTGGC GAGAAGCCCTACCGCTGTCCCCACTGCCCCTTTGCCTGCAGCAGCCTGGGCAACCTGAGGCGGCATCAGC GTACCCACGCAGGGCCCCCCACTCCTCCCTGCCCGACCTGTGGCTTCCGCTGCTGTACTCCACGACCAGC CCGGCCTCCCAGTCCCACAGAGCAGGAGGGGGCGGTGCCCCGGCGACCTGAAGATGCTCTGCTCCTTCCA GATTTGAGCCTCCATGTGCCACCAGGTGGTGCCAGTTTCCTGCCAGACTGTGGGCAGCTGCGGGGTGAAG GGGAGGGCCTCTGCGGGACTGGATCAGAACCACTGCCAGAGCTGCTATTCCCTTGGACCTGCCGGGGCTG TGGACAAGAGCTGGAGGAGGGTGAGGGTAGTCGGCTGGGAGCTGCCATGTGTGGGCGCTGCATGCGAGGA GAGGCTGGAGGGGGTGCCAGTGGGGGGCCCCAGGGCCCCAGTGACAAAGGCTTTGCCTGTAGCCTCTGCC CCTTTGCCACTCACTATCCCAACCACCTGGCCCGGCACATGAAGACACACAGTGGTGAGAAGCCCTTCCG CTGCGCCCGCTGTCCTTATGCCTCTGCTCATCTGGATAACCTGAAACGGCACCAGCGCGTCCATACAGGA GAGAAGCCCTACAAGTGCCCCCTCTGCCCTTATGCCTGTGGCAATCTGGCCAACCTCAAGCGTCATGGTC GCATCCACTCTGGTGACAAACCTTTTCGGTGTAGCCTTTGCAACTACAGCTGCAACCAGAGCATGAACCT CAAACGTCACATGCTGCGGCACACAGGCGAGAAGCCCTTCCGCTGTGCCACCTGCGCCTATACCACGGGC CACTGGGACAACTACAAGCGCCACCAGAAGGTGCATGGCCACGGTGGGGCAGGAGGGCCTGGTCTCTCTG CCTCTGAGGGCTGGGCCCCACCTCATAGCCCACCCTCTGTTTTGAGCTCTCGGGGCCCACCAGCCCTGGG GACTGCTGGCAGCCGGGCTGTCCACACAGACTCATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234222 representing NM_001201459 Red=Cloning site Green=Tags(s) MGFERDSEGDSLGARPGLPYGLSDDESGGGRALSAESEVEEPARGPGEARGERPGPACQLCGGPTGEGPC CGAGGPGGGPLLPPRLLYSCRLCTFVSHYSSHLKRHMQTHSGEKPFRCGRCPYASAQLVNLTRHTRTHTG EKPYRCPHCPFACSSLGNLRRHQRTHAGPPTPPCPTCGFRCCTPRPARPPSPTEQEGAVPRRPEDALLLP DLSLHVPPGGASFLPDCGQLRGEGEGLCGTGSEPLPELLFPWTCRGCGQELEEGEGSRLGAAMCGRCMRG EAGGGASGGPQGPSDKGFACSLCPFATHYPNHLARHMKTHSGEKPFRCARCPYASAHLDNLKRHQRVHTG EKPYKCPLCPYACGNLANLKRHGRIHSGDKPFRCSLCNYSCNQSMNLKRHMLRHTGEKPFRCATCAYTTG HWDNYKRHQKVHGHGGAGGPGLSASEGWAPPHSPPSVLSSRGPPALGTAGSRAVHTDSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001201459
ORF Size	1437 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001201459.1, NP_001188388.1
RefSeq Size	2091 bp
RefSeq ORF	1440 bp
Locus ID	130557
UniProt ID	Q8N8E2
Protein Families	Transcription Factors
MW	51.5 kDa
Gene Summary	The protein encoded by this gene is a possible transcriptional regulator involved in retinal development. Defects in this gene can be a cause of autosomal-recessive retinitis pigmentosa. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.