CALCOCO2 (NM_001261391) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NDP52
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234189 representing NM_001261391 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGACCATCAAAGATCCCCCCACATCAGCTGTCTTGCTGGATCACTGTCATTTCTCTCAGGTCA TCTTTAACAGTGTGGAGAAGTTCTACATCCCTGGAGGGGACGTCACATGTCATTATACCTTCACCCAGCA TTTCATCCCTCGTCGAAAGGATTGGATTGGCATCTTTAGATGTAGTCTTAATCAGACTATTCAACTCCTC ATCACCCCAGACACTGGTAGTATCTGGCACCAGGTGGGGTGGAAGACAACCCGTGAGTATTACACCTTCA TGTGGGTTACTTTGCCCATTGACCTAAACAACAAATCAGCTAAACAGCAGGAAGTCCAATTCAAAGCTTA CTACCTGCCCAAGGATGATGAGTATTACCAGTTCTGCTATGTGGATGAGGATGGTGTGGTCCGGGGAGCA AGTATTCCTTTCCAATTCCGTCCAGAAAATGAGGAAGACATCCTGGTTGTTACCACTCAGGGAGAGGTGG AAGAGATTGAGCAGCACAACAAGGAGCTTTGCAAAGAAAACCAGGAGCTGAAGGACAGCTGTATCAGCCT CCAGAAGCAGAACTCAGACATGCAGGCTGAGCTCCAAAAGAAGCAGGAGGAGCTAGAAACCCTACAGAGC ATCAATAAGAAGTTGGAACTGAAAGTGAAAGAACAGAAGGACTATTGGGAGACAGAGCTGCTTCAACTGA AAGAACAAAACCAGAAGATGTCCTCAGAAAATGAGAAGATGGGAATCAGAGTGGATCAGCTTCAGGCCCA GCTGTCAACTCAAGAGAAAGAAATGGAGAAGCTTGTTCAGGGAGATCAAGATAAGACAGAGCAGTTAGAG CAGCTGAAAAAGGAAAATGACCACCTCTTTCTCAGTTTAACTGAACAGAGGAAGGACCAGAAGAAGCTCG AGCAGACAGTGGAGCAAATGAAGCAGAATGAAACTACTGCAATGAAGAAACAACAGGAATTAATGGATGA AAACTTTGACCTGTCAAAAAGACTGAGTGAGAACGAAATTATATGTAATGCTCTGCAGAGACAGAAAGAG AGATTGGAAGGAGAAAATGATCTTTTGAAGAGGGAGAACAGCAGATTGCTCAGTTACATGGGTCTGGATT TTAATTCTTTGCCGTATCAAGTACCTACTTCAGATGAAGGAGGCGCAAGACAAAATCCAGGACTTGCCTA TGGAAACCCATATTCTGGTATCCAAGAAAGTTCTTCCCCCAGCCCGCTCTCCATCAAGAAATGCCCTATC TGCAAAGCAGATGATATTTGTGATCACACCTTGGAGCAACAGCAGATGCAGCCCCTTTGTTTCAATTGTC CAATTTGTGACAAGATCTTCCCAGCTACAGAGAAGCAGATCTTTGAAGACCACGTGTTCTGCCACTCTCT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234189 representing NM_001261391 Red=Cloning site Green=Tags(s) MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRCSLNQTIQLL ITPDTGSIWHQVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGA SIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQS INKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLE QLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKE RLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPI CKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001261391
ORF Size	1401 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001261391.2
RefSeq Size	3745 bp
RefSeq ORF	1404 bp
Locus ID	10241
UniProt ID	Q13137
MW	55.1 kDa
Gene Summary	This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

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